Synteny mapping in river buffalo

Abstract

The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP-YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology between cattle and river buffalo, comparative mapping predicts the localization of these syntenic groups on river buffalo Chromosomes (Chrs) :BBU7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been previously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.

Assignment of PCR markers to river buffalo chromosomes

“…The present study confirms syntenic conservation between cattle and buffalo as previously demonstrated [6,7,11,32]. Chromosomal conservation between cattle and buffalo has also been demonstrated, based on the banding homoeology between cattle and river buffalo chromosomes [22].…”

“…Therefore, inferences can be made concerning the expected location of markers in one species from information available in another species. Several genes previously assigned to cattle syntenic groups and/or chromosomes have been assigned to buffalo chromosomes [6,7,10,11,18,19,23,24,28].…”

A radiation hybrid map of river buffalo <i>(Bubalus bubalis)</i> chromosome 1 (BBU1)

“…According to previous studies and this latest map, all buffalo chromosome arms have homology to single bovine acrocentric chromosomes. Buffalo (BBU) chromosome 1 appears to be a fusion of Bos taurus (BTA) chromosome 1 and 27, BBU2 equals BTA2 and 23,BBU3 equals BTA8 and 19,BBU4 equals BTA5 and 28,and BBU5 equals BTA16 and 29 at the cytogenetic level with state of the art banding (El Nahas et al, 1996, 2001Iannuzzi et al, 2003). All the other chromosomes have a one-to-one correspondence between the two species.…”

ZOO-FISH and R-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes

“…ZOO-FISH studies using human chromosome paints have been reported in such domestic animals as cattle (Hayes, 1995;Solinas-Toldo et al, 1995;Chowdhary et al, 1996), pig Rettenberger et al, 1995), horse (Raudsepp et al, 1996), and cat (O'Brien et al, 1997), but they are still lacking in other economically important domestic species, such as the river buffalo, where only a few loci have been physically mapped using both somatic cell hybrid (El Nahas et al, 1996;De Hondt et al, 1997) and in situ hybridization techniques (Hassaname et al, 1993(Hassaname et al, , 1994Iannuzzi et al, 1997a-d). Furthermore, the difficulty in clearly identifying small acrocentric chromosomes, especially when banding techniques are applied with contracted chromosome preparations, lends greater importance to the ZOO-FISH analysis in river buffalo (Bubalus bubalis, BBU) chromosomes owing to the presence of marker chromosome pairs involving small, disputed cattle (Bos taurus, BTA) chromosomes in the standardized nomenclatures (Iannuzzi, 1996).…”