Localization of two calcium binding proteins, calbindin (28 kD) and parvalbumin (12 kD), in the vertebrate retina

Hamano, K.; Kiyama, Hiroshi; Emson, Piers C.; Manabe, R; Nakauchi, M; Tohyama, Masaya

doi:10.1002/cne.903020217

Cited by 139 publications

(109 citation statements)

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“…The location of these layers suggests that they are composed of amacrine cell processes. In agreement, parvalbumin expression was found in amacrine and displaced amacrine cells in chicken, rat, and dog (Hamano et al 1990). …”

Section: Neun a Marker For Neurons In The Gclsupporting

confidence: 64%

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A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Johansson

Eftekhari

Warfvinge

2010

J Histochem Cytochem.

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S U M M A R Y The pig is becoming an increasingly used non-primate model in experimental studies of human retinal diseases and disorders. The anatomy, size, and vasculature of the porcine eye and retina closely resemble their human counterparts, which allows for application of standard instrumentation and diagnostics used in the clinic. Despite many reports that demonstrate immunohistochemistry as a useful method for exploring neuropathological changes in the mammalian central nervous system, including the pig, the porcine retina has been sparsely described. Hence, to facilitate further immunohistochemical analysis of the porcine retina, we report on the successful use of a battery of antibodies for staining of paraformaldehyde-fixed cryosectioned retina. The following antibodies were evaluated for neuronal cells and structures: recoverin (cones and rods), Rho4D2 (rods), transducin-g (cones), ROM-1 (photoreceptor outer segments), calbindin (horizontal cells), PKC-a (bipolar cells), parvalbumin (amacrine and displaced amacrine cells), and NeuN (ganglion cells and displaced amacrines). For detecting synaptic connections in fiber layers, we used an antibody against synaptobrevin. For detecting retinal pigment epithelium, we studied antibodies against cytokeratin and RPE65, respectively. The glial cell markers used were bFGF (Müller cells and displaced amacrine cells), GFAP (Müller cells and astrocytes), and vimentin (Müller cells). Each staining effect was evaluated with regard to its specificity, sensitivity, and reproducibility in the identification of individual cells, specific cell structures, and fiber layers, respectively. The markers parvalbumin and ROM-1 were tested here for the first time for the porcine retina. All antibodies tested resulted in specific staining of high quality. In conclusion, all immunohistochemical protocols presented here will be applicable in fixed, cryosectioned pig retina. (J Histochem Cytochem 58:377-389, 2010)

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Section: Neun a Marker For Neurons In The Gclsupporting

confidence: 64%

“…Parvalbumin is another Ca 21 -binding protein (Hamano et al 1990), and its expression was restricted to amacrine cells in the INL and some displaced amacrine cells found in the ganglion cell layer (GCL). In addition, two fiber layers in the central part of the IPL stained positive for parvalbumin.…”

mentioning

confidence: 99%

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Johansson

Eftekhari

Warfvinge

2010

J Histochem Cytochem.

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“…Calbindin is a cone marker in a variety of species (Hamano et al 1990) and has been shown to label both large single cones and double cones in salamander (Deng et al 2001). Doublelabeling of CB1 receptors and calbindin was observed in the terminals of cone photoreceptors (Fig.…”

Section: Cannabinoid Cb1 Receptors Are Expressed In Both Rod and Conementioning

confidence: 91%

Cannabinoid Receptor Activation Differentially Modulates Ion Channels in Photoreceptors of the Tiger Salamander

Straiker

Sullivan

2003

Journal of Neurophysiology

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Staiker, Alex and Jane M. Sullivan. Cannabinoid receptor activation differentially modulates ion channels in photoreceptors of the tiger salamander. J Neurophysiol 89: 2647-2654, 2003; 10.1152/jn.00268.2002. Cannabinoid CB1 receptors have been detected in retinas of numerous species, with prominent labeling in photoreceptor terminals of the chick and monkey. CB1 labeling is well-conserved across species, suggesting that CB1 receptors might also be present in photoreceptors of the tiger salamander. Synaptic transmission in vertebrate photoreceptors is mediated by L-type calcium currents-currents that are modulated by CB1 receptors in bipolar cells of the tiger salamander. Presence of CB1 receptors in photoreceptor terminals would therefore be consistent with presynaptic modulation of synaptic transmission, a role seen for cannabinoids in other parts of the brain. Here we report immunohistochemical and electrophysiological evidence for the presence of functional CB1 receptors in rod and cone photoreceptors of the tiger salamander. The cannabinoid receptor agonist WIN 55212-2 enhances calcium currents of rod photoreceptors by 39% but decreases calcium currents of large single cones by 50%. In addition, WIN 55212-2 suppresses potassium currents of rods and large single cones by 44 and 48%, respectively. Thus functional CB1 receptors, present in the terminals of rod and cone photoreceptors, differentially modulate calcium and potassium currents in rods and large single cones. CB1 receptors are therefore well positioned to modulate neurotransmitter release at the first synapse of the visual system. I N T R O D U C T I O NCannabinoid CB1 receptors have been detected in the retinas of numerous species, including salamander, goldfish, chick, rat, mouse, monkey, and human (Straiker et al. 1999a,b;Yazulla et al. 1999) with a well-conserved pattern of staining that is particularly intense in both synaptic layers. In species for which double labeling has been performed-chick and monkey-outer plexiform layer staining is found to localize to photoreceptor terminals. CB1 is a member of the Gi/o-type G-protein-coupled receptor family and mediates a wide range of effects on ion channels, including voltage-dependent calcium and potassium channels (Deadwyler et al. 1995;Mackie and Hille 1992;Mackie et al. 1995;Mu et al. 1999). Retinal cannabinoid receptor activation has been shown to inhibit dopamine release in the guinea pig (Schlicker et al. 1996), to inhibit a delayed rectifier K ϩ current in ON cone bipolar cells of the goldfish (Yazulla et al. 2000), and to inhibit L-type calcium currents in bipolar cells of the tiger salamander (Straiker et al. 1999a). Three recent reports have described G-protein-coupled receptor-dependent modulation of ion channel function in photoreceptors of the tiger salamander by dopamine, adenosine, and somatostatin (Akopian et al. 2000;Stella and Thoreson 2000;Stella et al. 2002). These studies find that the calcium currents (I Ca ) of rods and large single cones respond differentially to activation ...

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“…The immunolocalization of CB has been described in retina and other structures of the visual pathways in different species (Hamano, 1990;Johnson, 1995;Mize, 1999). In our laboratory, it has been demonstrated that CB appears very early in the embryonic period of chick retina and shows changes in distribution along development and after hatching, which indicates that Ca 2+ homeostasis in retina is well regulated, probably to avoid excessive accumulation of the cation (Tolosa de Talamoni, 2002).…”

confidence: 71%

Effect of retinal ablation on the expression of calbindin D28k and GABA in the chick optic tectum

Barboza

Beltramino²,

Britto³

et al. 2009

Eur J Histochem

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The effect of retinal ablation on qualitative and quantitative changes of calbindin D28k and GABA expression in the contralateral optic tectum was studied in young chicks. Fifteen days old chicks had unilateral retinal ablation and after 7 or 15 days, calbindin expression was analyzed by Western blot and immunocytochemistry. Neuronal degeneration was followed by the amino-cupric silver technique. After 15 days, retinal lesions produced a significant decrease in calbindin immunostaining in the neuropil of layers 5-6 and in the somata of neurons from the layers 8 and 10 of the contralateral tectum, being this effect less marked at 7 days post-lesion. Double staining revealed that 50-60% of cells in the layers 8 and 10 were calbindin and GABA positive, 30-45% were only calbindin positive and 5-10% were only GABAergic neurons. Retinal ablation also produced a decrease in the GABA expression at either 7 or 15 days after surgery. At 7 days, dense silver staining was observed in the layers 5-6 from the optic tectum contralateral to the retinal ablation, which mainly represented neuropil that would come from processes of retinal ganglion cells. Tectal neuronal bodies were not stained with silver, although some neurons were surrounded by coarse granular silver deposits. In conclusion, most of calbindin molecules are present in neurons of the tectal GABAergic inhibitory circuitry, whose functioning apparently depends on the integrity of the visual input. A possible role of calbindin in the control of intracellular Ca 2+ in neurons of this circuit when the visual transmission arrives to the optic tectum remains to be studied.

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Localization of two calcium binding proteins, calbindin (28 kD) and parvalbumin (12 kD), in the vertebrate retina

Cited by 139 publications

References 20 publications

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Cannabinoid Receptor Activation Differentially Modulates Ion Channels in Photoreceptors of the Tiger Salamander

Effect of retinal ablation on the expression of calbindin D28k and GABA in the chick optic tectum

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