Effect of retinal ablation on the expression of calbindin D28k and GABA in the chick optic tectum

Barboza, Gabriela Díaz de; Beltramino, C.; Britto, Luiz Roberto Giorgetti de; Olmos, José de; Olmos, Soledad de; Pfeiffer, Carolin; Talamoni, Nori Tolosa de

doi:10.4081/848

Cited by 7 publications

(3 citation statements)

References 36 publications

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“…The exact mechanisms which control calbindin expression are not well understood. We could speculate that the reduced apoptosis during retinal development caused by capsazepine might increase the number of inputs to such cells, which may be important to the control calbindin expression (Diaz de Barboza et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

TRPV1 receptors modulate retinal development

Leonelli

Martins

Britto

2011

Intl J of Devlp Neuroscience

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We investigated the possible participation of TRPV1 channels in retinal apoptosis and overall development. Retinas from newborn, male albino rats were treated in vitro with capsazepine, a TRPV1 antagonist. The expression of cell cycle markers was not changed after TRPV1 blockade, whereas capsazepine reduced the number of apoptotic cells throughout the retina,increased ERK1/2 and p38 phosphorylation and slightly reduced JNK phosphorylation. The expression of BAD, Bcl-2, as well as integral and cleaved capsase-3 were similar in all experimental conditions. Newborn rats were kept for 2 months after receiving high doses of capsazepine. In their retinas, calbindin and parvalbumin protein levels were upregulated, but only the number of amacrine-like, parvalbumin-positive cells was increased. The numbers of calretinin, calbindin, ChAT, vimentin, PKC-alpha and GABA-positive cells were similar in both conditions. Protein expression of synapsin Ib was also increased in the retinas of capsazepine-treated rats. Calretinin, vimentin, GFAP, synapsin Ia, synaptophysin and light neurofilament protein levels were not changed when compared to control values. Our results indicate that TRPV1 channels play a role in the control of the early apoptosis that occur during retinal development, which might be dependent on MAPK signaling. Moreover, it seems that TRPV1 function might be important for neuronal and synaptic maturation in the retina.

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Section: Discussionmentioning

confidence: 99%

TRPV1 receptors modulate retinal development

Leonelli

Martins

Britto

2011

Intl J of Devlp Neuroscience

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“…The distribution of the calcium binding proteins has been examined in several species of birds: pigeon (Theiss et al, ; Chudinova et al, ); quail (Hilbig et al, ); chick (Pfeiffer and Britto, ; Díaz de Barboza et al, ; Metzger et al, ); and zebra finch (Braun et al, ; Heyers et al, ). From these analyses, despite differences in labeling of tectal layers and variations in methodology, one feature similar to what was noted above for mammals is present: the distribution of calbindin and parvalbumin are complimentary (Hilbig et al, ; Chudinova et al, ).…”

Section: Discussionmentioning

confidence: 99%

Calcium binding protein expression in the optic tectum of Alligator during development

Ruan

Pritz

2013

Developmental Neurobiology

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The onset and distribution of the calcium binding proteins, calretinin, calbindin, and parvalbumin, were examined in the optic tectum of Alligator mississipiensis embryos between Stages 18 and 26-28. The immunoreactivity of each calcium binding protein correlated well with the results from the Western blot experiments. In terms of onset and distribution, calretinin expressison was the most widespread of the three calcium binding proteins that were examined, and was also the earliest to be visualized. Calbindin expression occurred next, whereas parvalbumin expression was the most limited and appeared last. For small calretinin (+) neurons, the pattern of immunoreactivity during development was from inside to outside, whereas for the larger cells, it was from outside to inside. For calbindin immunoreactive cells in the superficial zone, the pattern was from outside to inside. The distribution of the parvalbumin immunopositive neurons did not change significantly over the time period examined. Similar data on other amniotes is limited. However, the pattern in Alligator shares some similarities with kittens in regards to the distribution of calbindin and parvalbumin in the developing superior colliculus.

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“…Postlesion argyrophilia is seen only after about 6 days after nerve lesion (Finger,1976,1978; Morita et al,1983), so transneuronal effects at 4 days would seem relatively fast. For example, transneuronal loss of CB in the optic tectum of chick only begins at 7 days postsection of the optic nerve (Diaz de Barboza et al,2003). Accordingly, we believe that the immediate decreases in both CaBP staining and Co 2+ filling are attributable to changes in the primary afferent fibers and terminals themselves.…”

Section: Discussionmentioning

confidence: 99%

Calcium‐fluxing glutamate receptors associated with primary gustatory afferent terminals in goldfish (Carassius auratus)

Huesa

Ikenaga

Böttger

et al. 2007

J of Comparative Neurology

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Presynaptic ionotropic glutamate receptors modulate transmission at primary afferent synapses in several glutamatergic systems. To test whether primary gustatory afferent fibers express Ca(2+)-permeable AMPA/kainate receptors, we utilized kainate-stimulated uptake of Co(2+) along with immunocytochemistry for the Ca(2+)-binding proteins (CaBPs) calbindin and calretinin to investigate the primary gustatory afferents in goldfish (Carassius auratus). In goldfish, the primary gustatory nucleus (equivalent to the gustatory portion of the nucleus of the solitary tract) includes the vagal lobe, which is a large, laminated structure protruding dorsally from the medulla. Kainate-stimulated uptake of Co(2+) (a measure of Ca(2+)-fluxing glutamate receptors) shows punctate staining distributed in the distinct laminar pattern matching the layers of termination of the primary gustatory afferent fibers. In addition, CaBP immunocytochemistry, which correlates highly with expression of Ca(2+)-permeable AMPA/kainate receptors, shows a laminar pattern of distribution similar to that found with kainate-stimulated cobalt uptake. Nearly all neurons of the vagal gustatory ganglion show Co(2+) uptake and are immunopositive for CaBPs. Transection of the vagus nerve proximal to the ganglion results in loss of such punctate Co(2+) uptake and of punctate CaBP staining as soon as 4 days postlesion. These results are consonant with the presence of Ca(2+)-fluxing glutamate receptors on the presynaptic terminals of primary gustatory terminals, providing an avenue for modulation of primary gustatory input.

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Effect of retinal ablation on the expression of calbindin D28k and GABA in the chick optic tectum

Cited by 7 publications

References 36 publications

TRPV1 receptors modulate retinal development

TRPV1 receptors modulate retinal development

Calcium binding protein expression in the optic tectum of Alligator during development

Calcium‐fluxing glutamate receptors associated with primary gustatory afferent terminals in goldfish (Carassius auratus)

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