Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-&lt;i&gt;ras&lt;/i&gt; 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Xu, Jinghua; Hardison, Ross C.

doi:10.1159/000132868

Cited by 20 publications

(10 citation statements)

References 2 publications

(2 reference statements)

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“…In rabbits, the a-globin gene family spans approximately 60 kb on chromosome 6 Xu and Hardison, 1991) and the b-globin gene family spans 45 kb on chromosome 1 (Margot et al, 1989;Xu and Hardison, 1989). If the observed patterns of variation at the HBA and HBB genes are attributable to hitchhiking associated with selection at linked loci, in each case the true target of selection is likely to be another closely linked globin gene.…”

Section: Functional Significance Of Haemoglobin Polymorphismmentioning

confidence: 99%

Evidence for contrasting modes of selection at interacting globin genes in the European rabbit (Oryctolagus cuniculus)

2008

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In hybrid zones between genetically differentiated populations, variation in locus-specific rates of introgression may reflect adaptation to different environments or adaptation to different genetic backgrounds. The European rabbit, Oryctolagus cuniculus, is well-suited to studies of such hybrid zone dynamics because it is composed of two genetically divergent subspecies that hybridize in a zone of secondary contact in central Iberia. A species-wide survey of allozyme variation revealed a broad range of locus-specific divergence levels (F ST ranged from 0 to 0.54, mean F ST ¼ 0.16). Interestingly, the two loci that fell at opposite ends of the distribution of F ST values, haemoglobin a-chain (HBA) and haemoglobin b-chain (HBB), encode interacting subunits of the haemoglobin protein. The contrasting patterns of spatial variation at these two loci could not be reconciled under a neutral model of population structure. The HBA gene exhibited higher-thanexpected levels of population differentiation, consistent with a history of spatially varying selection. The HBB gene exhibited lower-than-expected levels of population differentiation, consistent with some form of spatially uniform selection. Patterns of linkage disequilibrium and allele frequency variation do not appear to fit any simple model of two-locus epistatic selection.

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Section: Functional Significance Of Haemoglobin Polymorphismmentioning

confidence: 99%

Evidence for contrasting modes of selection at interacting globin genes in the European rabbit (Oryctolagus cuniculus)

2008

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“…In 1994, a genetic map containing 39 loci that were linked in ten autosomal linkage groups was published by Fox. Thus far, four of these linkage groups could be assigned to individual chromosomes; LGI has been mapped to OCU1 (Xu and Hardison, 1989), LGVII to OCU12 (Rogel-Gaillard et al 2001), LGVIII to OCU3 (Medrano andDutrillaux, 1986), and LGIX to OCU17 (Medrano and Dutrillaux, 1986). In order to assess the coverage of individual chromosomes by linkage groups, and to orient linkage groups along chromosomes, it is necessary to map genetic markers belonging to specific linkage groups on metaphase chromosomes.…”

confidence: 99%

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Zijlstra¹,

Haan²,

Korstanje

et al. 2002

Cytogenet Genome Res

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In order to improve the informativeness of the cytogenetic map of the rabbit genome, fourteen markers were regionally mapped to individual chromosomes. The localizations comprise eleven gene loci (PRLR, GHR, HK1, ACE, TF, 18S+28S rDNA, CYP2C4, PMP2, TCRB, ALOX15 and MT1) and three microsatellite loci (Sat13, Sol33 and D1Utr6). Five of the genes contain known microsatellite sequences. To achieve these localizations, homologous and heterologous small insert clones, and clones from a rabbit Bacterial Artificial Chromosome (BAC) library were used as probes for fluorescence in situ hybridization experiments. Results indicate that especially BAC clones are a valuable tool for cytogenetic mapping. Some of the genes were selected for mapping on the basis of human- rabbit comparative painting data, to achieve localizations on gene-poor rabbit chromosomes. Our data are, in general, in agreement with the human-rabbit comparative painting data. By mapping microsatellite sequences that have also been used in linkage studies, links are provided between the genetic and physical maps of the rabbit genome. Linkage groups I, VI and XI could be assigned to chromosomes 1, 5 and 3 respectively. Moreover, in this paper we give an overview of the current status of the rabbit cytogenetic map. This map now comprises 62 physically mapped genes, which are scattered over all autosomes, except chromosome 2, and the X chromosome.

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“…However, the coordinate expression of these two gene families is paradoxical. In both humans and rabbits, the two gene families are located on different chromosomes (17,67,68,70) in very different sequence contexts. The a-globin genes are in a very dense, GC-rich isochore (6), are embedded in CpG islands (21,29), and are unmethylated in all tissues examined (7).…”

mentioning

confidence: 99%

Nuclear protein-binding sites in a transcriptional control region of the rabbit alpha-globin gene.

1993

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The 5'-flanking and internal regions of the rabbit a-globin gene, which constitute a CpG island, are required for enhancer-independent expression in transfected cells. In this study, electrophoretic mobility shift assays revealed that a battery of nuclear proteins from both erythroid and nonerythroid cells bind specifically to these regulatory regions. Assays based on exonuclease m digestion, methylation interference, and DNase I footprinting identified sequences bound by proteins in crude nuclear extracts and by purified transcription factor Spl. In the 5' flank, recognition sites for the transcription factors a-IRP (positions -53 to -44 relative to the cap site), CP1 (-73 to -69), and Spl (-95 to -90) are bound by proteins in K562 cell nuclear extracts, as are three extended upstream regions. Two recognition sites for Spl in intron 1 are also bound both by proteins in crude nuclear extracts and by purified Spl. The sequences CCAC in intron 2 and C5 in the 3'-untranslated region also bind proteins. A major binding site found in exon 1, TATGGCGC, matches in sequence and methylation interference pattern the binding site for nuclear protein YY1, and binding is inhibited through competition by YY1-specific oligonucleotides. The protein-binding sites flanking and internal to the rabbit a-globin gene may form an extended promoter.

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Localization of the β-like globin gene cluster and the genes for parathyroid hormone and c-Harvey-<i>ras</i> 1 to region q14→q21 of rabbit chromosome 1 by in situ hybridization

Cited by 20 publications

References 2 publications

Evidence for contrasting modes of selection at interacting globin genes in the European rabbit (Oryctolagus cuniculus)

Evidence for contrasting modes of selection at interacting globin genes in the European rabbit (Oryctolagus cuniculus)

Fourteen chromosomal localizations and an update of the cytogenetic map of the rabbit

Nuclear protein-binding sites in a transcriptional control region of the rabbit alpha-globin gene.

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