Localization of the Goodpasture antigen by immunoelectron microscopy

Anti-Glomerular Basement Membrane Nephritis after Extracorporeal Shock Wave Lithotripsy

“…Studies of bovine and human GBM suggest that the Goodpasture antigen is present in the noncollagenous domain of collagen type IV. This antigen is located principally on the inner aspect of the lamina densa [10]. The etiology of the production of anti-GBM antibodies is unknown, although exposure to an environmental agent (for example, inhaled hydrocarbons, smoke or viruses) that damages alveolar walls and releases autoantigen may be involved [11][12][13][14].…”

The Vitreous

“…Hunt et al (1982) identified three antigenic fractions in noncollagenous portions of the glomerular basement membrane. Sisson et al (1982) have localized Goodpasture's antigen to the lamina rara interna of the glomerular basement membrane. Wieslander et al (1984) subsequently showed that the collagenase-resistant sequences of type IV collagen contain the Goodpasture antigen.…”

Pulmonary Injury Caused by Anti-Basement Membrane Antibodies

“…In fact, using highly refined immunoelectron microscopic techniques it has been possible to show that anti-GBM antibodies bind to determinants within the GBM which have a different distribution than that of the components named above. 17 Thus, anti-GBM antibodies bind to a component of the basement membrane which has not yet been formally identified.…”