Localization of the cytochrome cd1 and copper nitrite reductases in denitrifying bacteria

Coyne, Mark S.; Arunakumari, Alahari; Pankratz, H. Stuart; Tiedje, James M.

doi:10.1128/jb.172.5.2558-2562.1990

Cited by 65 publications

(57 citation statements)

References 24 publications

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“…These compounds are routinely used in culturing microbes and testing for metal requirements in enzymes (e.g. Coyne et al 1989, Sillanpaa & Aimo 1996. HAs are natural metal-complexing compounds found in normal lake systems having comparatively low affinities for trace metals in high ionic strength solutions.…”

Section: Methodsmentioning

confidence: 99%

What limits bacterial production in the suboxic region of permanently ice-covered Lake Bonney, Antarctica?

Ward¹,

Granger²,

Maldonado³

et al. 2003

Aquat. Microb. Ecol.

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Bacterial production assays (thymidine incorporation rates) were used to evaluate the activity of heterotrophic bacteria at the chemocline region in both the East (ELB) and West (WLB) Lobes of permanently ice-covered Lake Bonney, in the Taylor Valley of Antarctica. The magnitude of activity varied dramatically within the depth interval of 1 to 2 m from moderate to very low levels below the chemocline, especially in the East Lobe, where chemical distributions indicate the absence of a normally functioning nitrogen cycle. Several parameters (e.g. addition of nutrients or chelators, dilution) were manipulated in incubation experiments in order to identify factors that would enhance activity in the suboxic deep waters of the East Lobe. Activity, in terms of thymidine incorporation, was consistently detected in the deep-water communities, implying that, although the water may be 'toxic', the cells remain viable. None of the treatments resulted in consistent enhancement of thymidine incorporation rates in samples from below the chemocline. Bacterial populations above the chemocline appear to be phosphorus-limited. The nature of the limitation, toxicity or inhibition that limits bacterial activity in the suboxic waters has not been identified.

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Section: Methodsmentioning

confidence: 99%

What limits bacterial production in the suboxic region of permanently ice-covered Lake Bonney, Antarctica?

Ward¹,

Granger²,

Maldonado³

et al. 2003

Aquat. Microb. Ecol.

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“…1) Denitrification is a kind of anaerobic respiration in which nitrate (or nitrite) is reduced to form gaseous nitrogen compounds such as dinitrogen (N 2 ) and nitrous oxide (N 2 O). [2][3][4] Anaerobic respiration has long been believed to be a characteristic of prokaryotes. Following the discovery of denitrifying activity in the filamentous fungus Fusarium oxysporum, 5) such activities have been known to be widely distributed among eukaryotes, fungi and yeasts.…”

mentioning

confidence: 99%

Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA;CYP55A5) Encoding Nitric Oxide Reductase fromAspergillus oryzae

Kaya

Matsumura

Higashida

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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“…DDC is somewhat preferential for copper and is often used to chelate copper when testing for its requirement in enzymes (e.g. Coyne et al 1989). In contrast, DTPA is a more general synthetic metal chelator having differing affinities for a range of bioactive metals.…”

Section: Methodsmentioning

confidence: 99%

Denitrification in the hypolimnion of permanently ice-covered Lake Bonney, Antarctica

Ward¹,

Granger²,

Maldonado³

et al. 2005

Aquat. Microb. Ecol.

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The distribution of denitrification was investigated in the hypolimnion of the east and west lobes of permanently ice-covered Lake Bonney, Taylor Valley, Antarctica. Anomalously high concentrations of dissolved inorganic nitrogen (DIN; nitrate, nitrite, ammonium and nitrous oxide) in the oxygen-depleted hypolimnion of the east lobe of the Lake implied that denitrification is or was active in the west, but not in the east lobe. While previous investigations reported no detectable denitrification in the east lobe, we measured active denitrification in samples from both the east and west lobes. In the west lobe, measured denitrification rates exhibited a maximum at the depth of the chemocline and denitrification was not detectable in either the oxic surface waters or in the deep water where nitrate was absent. In the east lobe, denitrification was detected below the chemocline, at the depths where ammonium, nitrate, nitrite and nitrous oxide are all present at anomalously high levels. Trace metal availability was manipulated in incubation experiments in order to determine whether trace metal toxicity in the east lobe could explain the difference in nitrogen cycling between the 2 lobes. There were no consistent stimulatory effects of metal chelators or nutrient addition on the rate of denitrification in either lobe, so that the mechanisms underlying the unusual N cycle of the east lobe remain unknown. We conclude that all the ingredients necessary to allow denitrification to occur are present in the east lobe. However, even though denitrification could be detected under certain conditions in incubations, denitrification is inhibited under the in situ conditions of the lake.

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Localization of the cytochrome cd1 and copper nitrite reductases in denitrifying bacteria

Cited by 65 publications

References 24 publications

What limits bacterial production in the suboxic region of permanently ice-covered Lake Bonney, Antarctica?

What limits bacterial production in the suboxic region of permanently ice-covered Lake Bonney, Antarctica?

Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA;CYP55A5) Encoding Nitric Oxide Reductase fromAspergillus oryzae

Denitrification in the hypolimnion of permanently ice-covered Lake Bonney, Antarctica

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