Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA;CYP55A5) Encoding Nitric Oxide Reductase fromAspergillus oryzae

Kaya, Masahiko; Matsumura, Kazuaki; Higashida, Katsuya; Hata, Yutaka; Kawato, Akitsugu; Abe, Yasuhisa; Akita, Osamu; Takaya, Naoki; Shoun, Hirofumi

doi:10.1271/bbb.68.2040

Cited by 27 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, some A. oryzae genes for NO metabolism, namely a mitochondrial copper-containing nitrite reductase (nirK), a NO reductase (nicA), and a cytosolic NO-detoxifying flavohemoprotein (fhb1) were also up-regulated at low O 2 levels (Kaya et al 2004;Nakanishi et al 2010;Zhou et al 2011). These findings lead the authors to speculate about a possible role of fungal denitrification (reduction of nitrate to nitrous oxide) in hypoxic survival and NAD + recycling of A. oryzae.…”

Section: Gene and Protein Expression During Hypoxiamentioning

confidence: 97%

Insights into the cellular responses to hypoxia in filamentous fungi

2015

View full text Add to dashboard Cite

Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are confronted with low levels of atmospheric oxygen. Transcriptome and proteome studies on the hypoxic response of filamentous fungi revealed significant alteration of the gene expression and protein synthesis upon hypoxia. These analyses discovered not only common but also species-specific responses to hypoxia with regard to NAD(+) regeneration systems and other metabolic pathways. A surprising outcome was that the induction of oxidative and nitrosative stress defenses during oxygen limitation represents a general trait of adaptation to hypoxia in many fungi. The interplay of these different stress responses is poorly understood, but recent studies have shown that adaptation to hypoxia contributes to virulence of pathogenic fungi. In this review, results on metabolic changes of filamentous fungi during adaptation to hypoxia are summarized and discussed.

show abstract

Section: Gene and Protein Expression During Hypoxiamentioning

confidence: 97%

Insights into the cellular responses to hypoxia in filamentous fungi

2015

View full text Add to dashboard Cite

show abstract

“…Unfortunately, there are still no tools for direct analysis of fungal denitrification gene abundance in soil. Currently, five different genes coding for key enzymes of fungal denitrification pathway (cytochrome P450nor) have been described, sharing only about 60% homology at the amino acid level (Kaya et al, 2004). Due to relatively low homology between the sequences, PCR amplification of these genes using designed universal primers was reported as difficult and inaccurate (Manohar, 2010).…”

Section: Association Of N 2 O-producing Isolates With Six Sections Ofmentioning

confidence: 99%

Nitrous oxide productivity of soil fungi along a gradient of cattle impact

Jirout

2015

Fungal Ecology

View full text Add to dashboard Cite

“…Unlike bacterial denitrification, the fungal denitrification pro-cess relies mainly on the activity of two enzymes, a dissimilatory nitrite reductase (dNir), encoded by the nirK gene, and a nitric oxide reductase (Nor), encoded by the P450nor gene, which were either partially or completely purified from Fusarium oxysporum (18,19), Cylindrocarpon tonkinense (20), and Aspergillus oryzae (21), the most studied denitrifying fungi thus far. The fungal dNir is a copper-containing nitrite reductase that closely resembles its bacterial counterpart (22).…”

mentioning

confidence: 99%

Novel P450nor Gene Detection Assay Used To Characterize the Prevalence and Diversity of Soil Fungal Denitrifiers

Novinscak

Zebarth

Burton

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Denitrifying fungi produce nitrous oxide (N 2 O), a potent greenhouse gas, as they generally lack the ability to convert N 2 O to dinitrogen. Contrary to the case for bacterial denitrifiers, the prevalence and diversity of denitrifying fungi found in the environment are not well characterized. In this study, denitrifying fungi were isolated from various soil ecosystems, and novel PCR primers targeting the P450nor gene, encoding the enzyme responsible for the conversion of nitric oxide to N 2 O, were developed, validated, and used to study the diversity of cultivable fungal denitrifiers. This PCR assay was also used to detect P450nor genes directly from environmental soil samples. Fungal denitrification capabilities were further validated using an N 2 O gas detection assay and a PCR assay targeting the nirK gene. A collection of 492 facultative anaerobic fungi was isolated from 15 soil ecosystems and taxonomically identified by sequencing the internal transcribed spacer sequence. Twenty-seven fungal denitrifiers belonging to 10 genera had the P450nor and the nirK genes and produced N 2 O from nitrite. N 2 O production is reported in strains not commonly known as denitrifiers, such as Byssochlamys nivea, Volutella ciliata, Chloridium spp., and Trichocladium spp. The prevalence of fungal denitrifiers did not follow a soil ecosystem distribution; however, a higher diversity was observed in compost and agricultural soils. The phylogenetic trees constructed using partial P450nor and nirK gene sequences revealed that both genes clustered taxonomically closely related strains together. IMPORTANCE A PCR assay targeting the P450nor gene involved in fungal denitrification was developed and validated. The newly developedP450nor primers were used on fungal DNA extracted from a collection of fungi isolated from various soil environments and on DNA directly extracted from soil. The results indicated that approximatively 25% of all isolated fungi possessed this gene and were able to convert nitrite to N 2 O. All soil samples from which denitrifying fungi were isolated also tested positive for the presence of P450nor. The P450nor gene detection assay was reliable in detecting a large diversity of fungal denitrifiers. Due to the lack of homology existing between P450nor and bacterial denitrification genes, it is expected that this assay will become a tool of choice for studying fungal denitrifiers.

show abstract

Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA;CYP55A5) Encoding Nitric Oxide Reductase fromAspergillus oryzae

Cited by 27 publications

References 30 publications

Insights into the cellular responses to hypoxia in filamentous fungi

Insights into the cellular responses to hypoxia in filamentous fungi

Nitrous oxide productivity of soil fungi along a gradient of cattle impact

Novel P450nor Gene Detection Assay Used To Characterize the Prevalence and Diversity of Soil Fungal Denitrifiers

Contact Info

Product

Resources

About