Localization of peanut (Arachis hypogaea) root lectin (PRA II) on root surface and its biological significance

Kalsi, Gurpreet; Babu, C. R.; Das, Rakha H.

doi:10.1007/bf00731867

Cited by 16 publications

(7 citation statements)

References 24 publications

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“…Research on Trifoliin A largely contributed to the legume lectin story (Dazzo and Truchet, 1983), but this protein and the corresponding gene await further characterization. Other interesting lectins are the 4-methyl-glucuronic acidbinding lectin from soybean, which may interact with surface saccharides from Bvadyrhizobium elkani (DombrinkKurtzman et al, 1983), and PRAII, a Glc-binding lectin from the roots of peanut, which specifically agglutinates peanut rhizobia (Kalsi et al, 1995).…”

Section: Other Root Lectinsmentioning

confidence: 99%

Root Lectins and Rhizobia

Kijne¹,

Bauchrowitz²,

Diaz³

1997

Plant Physiology

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Section: Other Root Lectinsmentioning

confidence: 99%

Root Lectins and Rhizobia

Kijne¹,

Bauchrowitz²,

Diaz³

1997

Plant Physiology

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“…triJolii suggest LPS to be strain specific antigenic determinants (13). Our recent studies on LPS isolated from the peanut specific strains indicated that these molecules interact specifically with the peanut root lectin PRA II (6,14,15). The diversity in composition, structure and antigenic determinants of the LPS from various rhizobia and our studies on interaction of LPS with PRAII paved the way to characterize the LPS from Bradyrhizobium specific for the peanut plant.…”

mentioning

confidence: 99%

Partial characterization of the lipopolysaccharides of peanut specific Bradyrhizobium strains

Jayaraman¹,

Das²

1998

IUBMB Life

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Immunological studies of the LPS of R.leguminosarum bv. triJolii suggest LPS to be strain specific antigenic determinants (13). Our recent studies on LPS isolated from the peanut specific strains indicated that these molecules interact specifically with the peanut root lectin PRA II (6,14,15). The diversity in composition, structure and antigenic determinants of the LPS from various rhizobia and our studies on interaction of LPS with PRAII paved the way to characterize the LPS from Bradyrhizobium specific for the peanut plant. In this report, we describe the isolation and partial characterization of LPS molecules from peanut specific Bradyrhizobium strains~ MATERIALS AND METHODSThe peanut specific Bradyrhizobial strains NC 92 and IGR 92, pea specific rhizobial strain P 14-93 and soybean specific B.japonicum were obtained from the Department of Microbiology, Indian Agricultural Research Institute, New Delhi.The peanut specific strains IGR 40 and IGR 6 were obtained from the National Research Centre for Groundnut, Junagarb, Gujarat, India. The bacteria were grown in 10 1 batches at 28 o C in a yeast extract mannitol medium (pH 6.8) containing 0.2 g MgSO4.7I-t20, 0.1 g NaCI, 1.0 g mannitol, 0.4 g yeast extract (per litre). Cells were harvested in the late exponential phase and collected by centrifugation at 7000g for 30 rain at 40 C. The yield was 2-5g wet weight/1 of culture. The optical density (OD) at 620 nm at the time of harvest was 1.5 for all strains. Prior to LPS extraction, the bacterial pellet was suspended in a fresh yeast extract-manitol medium and centrifuged to remove any adsorbed EPS from the pellet. The pellet was then suspended in phosphate buffered saline (PBS, pH 7.2) and centrifuged to remove any capsular polysaccharides. The LPS of the bacterial cell wall was isolated by the hot water-phenol as described by Westphal and Jann (16). The lyophilized powder was designated as native LPS or LPS . Hexose and heptose contents were determined by orcinol-sulphuric acid (17) and cysteine-sulphuric acid methods (18) respectively. 2-keto-3-deoxyoctonic acid was assayed by thiobarbituric acid method (19), while uronic acid was assayed by the carbazole method (20), using commercially available standards. Discontinuous slab gel electrophoresis (13%) of LPS with SDS (0.1%) were performed and the gels were stained for LPS by the silver staining procedure of Hitchcock and Brown( 2 l). The LPS of NC 92 strain was analyzed by gel filtration chromatography on a Sepharose 4B column. The lyophilized LPS powder was dissolved in 100mM EDTA-300raM triethylamine (TEA, pH 7.0) with 0.02% sodium azide at a concentration of 10mg/ml and applied onto a Sepharose 4B column (85cm x 1. lcm) preequilibrated with 10mM EDTA -300mM TEA at a flow rate of 12ml/laour. The eluted fractions (2ml) were assayed for Kdo and hexose. All the hexose and Kdo positive fractions were pooled, dialyzed against distilled water to remove EDTA and TEA and then concentrated. The polysaccharide region of the LPS was separated from lipid A by mild acid hy...

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“…In peanut roots, lectins have been found that bind α-as well as β-glycosidically linked glucose residues [Kalsi et al, 1992[Kalsi et al, , 1995Das et al, 1994;Singh and Das, 1994;Singh et al, 1997]. This is in marked contrast to other leguminous lectins which bind glucose and mannose but exclusively their a-anomeric forms.…”

Section: Extrinsic Rolesmentioning

confidence: 41%