Localization of neurokinin A and chromogranin A immunoreactivity in the developing porcine adrenal medulla

Wang, J. M.; Ridder, E. F. M. M. De; Potter, W.P. De; Weyns, A.

doi:10.1007/bf00160056

Cited by 11 publications

(4 citation statements)

References 22 publications

(4 reference statements)

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“…Several studies have demonstrated CgA immunostaining in the early migratory neuroblast population in fetal chick, bovine, and human, 3.5, 35, and 42 days gestation, respectively (Ehrhart-Bornstein et al 1996;Molenaar et al 1990;Sanchez-Montesinos et al 1996;Totzauer et al 1995). In this study the least developed porcine fetuses evaluated were F24 where CgA immunostaining was detected in migrating neuroblast cells 14.5 days earlier than previously reported (Wang et al 1994). This study has also demonstrated that CgA is subject to post-translational processing to generate WE-14 in a population of migrating neuroblasts at F24.…”

Section: Discussioncontrasting

confidence: 59%

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Barkatullah

Pogue

Depreitere

et al. 2001

Histochem Cell Biol

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Immunohistochemical investigation of the post-translational processing of chromogranin A (CgA) to generate WE-14 in the sympathoadrenal cell lineage of the developing porcine fetus (F) detected intense CgA and weak WE-14 immunoreactivity in migrating neuroblast cells of the diffuse sympathetic ganglia adjacent to the dorsal aorta and projecting toward the cortical mass at F24-27. F37-42; WE-14 immunoreactivity was detected in chromaffinoblasts at the periphery of the developing cortex and at F54-56 days gestation WE-14 immunoreactivity was detected in a large population of central medullary cells. From F74 to F76 days and thereafter the number of cells exhibiting intense WE-14 immunostaining decreased, and the majority of chromaffin cells exhibited uniform weak WE-14 immunostaining. At postnatal day 1 (P1) intense WE-14 immunoreactivity was primarily confined to clusters of chromaffin cells with weak immunostaining in the general population. The transitory neuroblasts, chromaffinoblasts, and maturing chromaffin cell population exhibited uniform intense CgA immunostaining through gestation and after birth. Additional observations detected intense CgA and WE-14 immunostaining in extrachromaffin tissue at P1 and in neuronal-like cells in vessels of the aortic arch at F37. This study has demonstrated that CgA is post-translationally processed to generate WE-14 during early fetal development in the migrating progenitor cells of the porcine sympathoadrenal lineage.

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Section: Discussioncontrasting

confidence: 59%

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Barkatullah

Pogue

Depreitere

et al. 2001

Histochem Cell Biol

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“…CHGA protein was found in chromaffin cells through all stages in the developing porcine and bovine adrenal medulla (Wang et al 1994, Totzauer et al 1995. CHGA and its derived peptides were also detected in the gastro-entero-pancreatic system of rat and porcine (Kent & Coupland 1989), as well as in the secretory-specific cells of the hypophyseal pars tuberalis of chicken (Kameda et al 1998).…”

Section: Discussionmentioning

confidence: 98%

“…Several animal models (chicken, bovine, and rat) have been employed for studying the developmental and anatomic distribution of chga-positive cells in these species. The existing results mainly indicate the expression of CHGA protein in adrenal medulla and gastro-entero-pancreatic system that abounds with various endocrine cells (Kent & Coupland 1989, Mahata et al 1993, Wang et al 1994, Totzauer et al 1995, Kameda et al 1998. The distribution of Chga transcripts in the rat brain has also been reported (Mahata et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Spatio-temporal expression of chromogranin A during zebrafish embryogenesis

Xie¹,

Wang²,

Liu³

et al. 2008

Journal of Endocrinology

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Chromogranin A (CHGA), a protein participating in the biogenesis of dense core secretory granules in various neuroendocrine tissues, plays a critical role in the release of hormones/peptides and the pathogenesis of pheochromocytoma. However, little is known about the developmental origin of CHGA-expressing cells during embryogenesis. Here, we report the structural characterization and spatiotemporal expression pattern of zebrafish (Danio rerio) ortholog of mammalian CHGA. The earliest expression of chga transcripts was observed at 16 h post fertilization in the developing cranial ganglia as six distinct cellular masses arranged bilaterally as strings of beads in the dorsal root ganglia (DRG) precursors along the dorsal trunk. With development advancing, the chga transcripts were expressed abundantly in diencephalon, mesencephalon, and rhombencephalon as well as in the DRG. Interestingly, double in situ hybridization assay of chga with genes expressed in pronephros (Wilms' tumor suppressor 1, wt1), adrenal cortex (side-chain cleavage enzyme, scc), and sympathoadrenal neuron/chromaffin cell (dopamineb-hydroxylase, dbh), respectively, showed that the chgaexpressing cells are spatially separated from wt1-, scc-, and dbh-positive cell populations during early embryonic development. The pronephros region does not express chga even up to 7 days post fertilization, while chga positive-staining cells bind in the brain and DRG, indicating that chga may play an important role in nervous system development during the early embryonic stages.

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“…neurofilament and SCG10 [ 3 , 7 ]. While chromogranin A [ 8 ] and Phenylethanolamine-N-Methyltransferase (PNMT) [ 1 ], which is responsible for adrenaline synthesis, are considered as typical chromaffin cell markers, both can also be detected in other sympathetic structures [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The role of the Notch signalling pathway in regulating the balance between neuronal and nonneuronal cells in sympathetic ganglia and the adrenal gland

Shtukmaster

Huber

2023

PLoS ONE

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Sympathetic neurons and endocrine chromaffin cells of the adrenal medulla are catecholaminergic cells that derive from the neural crest. According to the classic model, they develop from a common sympathoadrenal (SA) progenitor that has the ability to differentiate into both sympathetic neurons and chromaffin cells depending on signals provided by their final environment. Our previous data revealed that a single premigratory neural crest cell can give rise to both sympathetic neurons and chromaffin cells, indicating that the fate decision between these cell types occurs after delamination. A more recent study demonstrated that at least half of chromaffin cells arise from a later contribution by Schwann cell precursors. Since Notch signalling is known to be implicated in the regulation of cell fate decisions, we investigated the early role of Notch signalling in regulating the development of neuronal and non-neuronal SA cells within sympathetic ganglia and the adrenal gland. To this end, we implemented both gain and loss of function approaches. Electroporation of premigratory neural crest cells with plasmids encoding Notch inhibitors revealed an elevation in the number of SA cells expressing the catecholaminergic enzyme tyrosine-hydroxylase, with a concomitant reduction in the number of cells expressing the glial marker P0 in both sympathetic ganglia and adrenal gland. As expected, gain of Notch function had the opposite effect. Numbers of neuronal and non-neuronal SA cells were affected differently by Notch inhibition depending on the time of its onset. Together our data show that Notch signalling can regulate the ratio of glial cells, neuronal SA cells and nonneuronal SA cells in both sympathetic ganglia and the adrenal gland.

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Localization of neurokinin A and chromogranin A immunoreactivity in the developing porcine adrenal medulla

Cited by 11 publications

References 22 publications

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Spatio-temporal expression of chromogranin A during zebrafish embryogenesis

The role of the Notch signalling pathway in regulating the balance between neuronal and nonneuronal cells in sympathetic ganglia and the adrenal gland

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