Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Abstract

Immunohistochemical investigation of the post-translational processing of chromogranin A (CgA) to generate WE-14 in the sympathoadrenal cell lineage of the developing porcine fetus (F) detected intense CgA and weak WE-14 immunoreactivity in migrating neuroblast cells of the diffuse sympathetic ganglia adjacent to the dorsal aorta and projecting toward the cortical mass at F24-27. F37-42; WE-14 immunoreactivity was detected in chromaffinoblasts at the periphery of the developing cortex and at F54-56 days gestation WE-14 immunoreactivity was detected in a large population of central medullary cells. From F74 to F76 days and thereafter the number of cells exhibiting intense WE-14 immunostaining decreased, and the majority of chromaffin cells exhibited uniform weak WE-14 immunostaining. At postnatal day 1 (P1) intense WE-14 immunoreactivity was primarily confined to clusters of chromaffin cells with weak immunostaining in the general population. The transitory neuroblasts, chromaffinoblasts, and maturing chromaffin cell population exhibited uniform intense CgA immunostaining through gestation and after birth. Additional observations detected intense CgA and WE-14 immunostaining in extrachromaffin tissue at P1 and in neuronal-like cells in vessels of the aortic arch at F37. This study has demonstrated that CgA is post-translationally processed to generate WE-14 during early fetal development in the migrating progenitor cells of the porcine sympathoadrenal lineage.

Chromogranina A i jej rola w patogenezie cukrzycy

“…Various diseases, including non-oncological gastrointestinal, cardiovascular, and renal conditions, and several tumour types, may also increase the serum levels [33,140] PRACE ORYGINALNE WE-14 (the abbreviation comes from N-and Cterminal amino acids and the length of the molecule) was isolated first from human metastatic hepatic cells originating from an ileal carcinoid tumour in 1992 [41], and also proved to be a CgA cleavage product. The peptide was later isolated from human, bovine, and NOD mouse pancreatic beta cells, flask-shaped endocrine cells of intestinal crypts, and porcine adrenal chromaffin cells [11,42,43].…”

Localisation of WE‐14 immunoreactivity in the developing mouse limbo‐corneal nerve net

“…Immunocytochemical control studies were performed using 4% (w/v) PFA fixed cryoprotected adult tissue sections (30 m). This included the liquid phase preabsorption of the WE-14 antiserum with synthetic human WE-14 and the Cterminal hexapeptide antigen (Y°KELTAE) as described previously (Barkatullah et al 1997(Barkatullah et al , 2001Norlen et al, 2001) and replacement of the primary antiserum with non-immune rabbit serum and the omission of the fluorescein isothiocynate tagged swine anti-rabbit sera. Unless otherwise stated, all chemicals were purchased from Sigma Chemical Company, Poole, UK.…”

“…It is generated in a subpopulation of adult rat neuroendocrine cells (Curry et al, 1991) and to varying degrees in human neuroendocrine neoplasia (Gleeson et al, 1996;Heaney et al, 2000). Developmental studies have shown that WE-14 is generated at an early stage of endocrine gland generation in the rat and pig (Barkatullah et al, 1997(Barkatullah et al, , 2001 and that it has an ancient lineage (Curry et al, 2002).…”

What's new in histochemistry and cell biology?

“…The importance of chromogranin A (CgA)as a prohormone for the neuropeptide WE-14 was studied in the sympathoadrenal cell lineage of the developing porcine fetus (Barkatullah et al 2001). Evidence was obtained to indicate that CgA was posttranslationally processed to generate WE-14 during early fetal development in the migrating progenitor cells of the porcine sympathoadrenal lineage.…”