Localization of neonatal Fc receptor for IgG in aggregated lymphoid nodules area in abomasum of Bactrian camels (Camelus bactrianus) of different ages

Zhang, Wangdong; Wang, Wenhui; Li, Shuxian; Jia, Shuai; Zhang, Xuefeng; Cao, Tingting

doi:10.1186/s12917-016-0847-9

Cited by 9 publications

(12 citation statements)

References 54 publications

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“…In general, several studies show the involvement of FcRn in IgG transport in different tissues and cell types [ 15 , 16 , 17 , 18 , 19 ]. Thus, FcRn was also found to be expressed in the BBB.…”

Section: Introductionmentioning

confidence: 99%

Impact of Glycosylation and Species Origin on the Uptake and Permeation of IgGs through the Nasal Airway Mucosa

et al. 2020

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Although we have recently reported the involvement of neonatal Fc receptor (FcRn) in intranasal transport, the transport mechanisms are far from being elucidated. Ex vivo porcine olfactory tissue, primary cells from porcine olfactory epithelium (OEPC) and the human cell line RPMI 2650 were used to evaluate the permeation of porcine and human IgG antibodies through the nasal mucosa. IgGs were used in their wild type and deglycosylated form to investigate the impact of glycosylation. Further, the expression of FcRn and Fc-gamma receptor (FCGR) and their interaction with IgG were analyzed. Comparable permeation rates for human and porcine IgG were observed in OEPC, which display the highest expression of FcRn. Only traces of porcine IgGs could be recovered at the basolateral compartment in ex vivo olfactory tissue, while human IgGs reached far higher levels. Deglycosylated human IgG showed significantly higher permeation in comparison to the wild type in RPMI 2650 and OEPC, but insignificantly elevated in the ex vivo model. An immunoprecipitation with porcine primary cells and tissue identified FCGR2 as a potential interaction partner in the nasal mucosa. Glycosylation sensitive receptors appear to be involved in the uptake, transport, but also degradation of therapeutic IgGs in the airway epithelial layer.

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Section: Introductionmentioning

confidence: 99%

Impact of Glycosylation and Species Origin on the Uptake and Permeation of IgGs through the Nasal Airway Mucosa

et al. 2020

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“…However, the studies about the different mucosal immune inductive sites of gastrointestinal tract has only received few attentions. Hence, based on our previous histological and structural studies of ALNA and ileal PPs in Bactrian camels [16,18,[21][22][23], 16S rDNA-Illumina Miseq sequencing was used to comparatively analyze the bacteria community characteristics in ALNA and ileal PPs of Bactrian camels, moreover, the biological information hidden in them were revealed in this study.…”

Section: Introductionmentioning

confidence: 99%

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

Zhang

Yao

et al. 2020

PLoS ONE

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The microbial communities colonize the mucosal immune inductive sites could be captured by hosts, which could initiate the mucosal immune responses. The aggregated lymphoid nodule area (ALNA) and the ileal Payer's patches (PPs) in Bactrian camels are both the mucosal immune inductive sites of the gastrointestinal tract. Here, the bacteria community associated with the ALNA and ileal PPs were analyzed using of 16S rDNA-Illumina Miseq sequencing. The mutual dominant bacterial phyla at the two sites were the Bacteroidetes, Firmicutes, Verrucomicrobia and Proteobacteria, and the mutual dominant genus in both sits was Prevotella. The abundances of the Fibrobacter, Campylobacter and RFP12 were all higher in ALNA than in ileal PPs. While, the abundances of the 5-7N15, Clostridium, and Escherichia were all higher in ileal PPs than in ALNA. The results suggested that the host's intestinal microenvironment is selective for the symbiotic bacteria colonizing the corresponding sites, on the contrary, the symbiotic bacteria could impact on the physiological functions of this local site. In ALNA and ileal PPs of Bactrian camel, the bacteria which colonized different immune inductive sites have the potential to stimulate different immune responses, which is the result of the mutual selection and adaptation between microbial communities and their host.

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“…This systematic review focused on the role of FcRn in the nasal administration of biologics. For almost twenty years, researchers have demonstrated the involvement of FcRn in IgG transport in various tissues and cell types [ 7 , 11 , 32 , 70 , 71 ]. Accordingly, all of the studies included in this review demonstrated efficient transcytosis [ 9 , 15 , 16 , 26 , 27 , 28 , 29 , 35 , 46 , 61 , 69 ] of all of the biologics tested, irrespective of their molecular weight or the dose administered; therefore, they indicate that nasal administration may represent an efficient administration route for biologics for inducing both local and systemic effects.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent work demonstrated that intranasally applied low molecular weight peptides, e.g., oxytocin (1 kDa), readily access the CSF of rodents [ 71 , 92 ], monkeys [ 93 ] and humans [ 81 ]. In addition, the nasal route for biologics administration is not always successful.…”

Section: Discussionmentioning

confidence: 99%

FcRn as a Transporter for Nasal Delivery of Biologics: A Systematic Review

Fieux

Quellec

Bartier

et al. 2021

IJMS

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FcRn plays a major role in regulating immune homeostasis, but it is also able to transport biologics across cellular barriers. The question of whether FcRn could be an efficient transporter of biologics across the nasal epithelial barrier is of particular interest, as it would allow a less invasive strategy for the administration of biologics in comparison to subcutaneous, intramuscular or intravenous administrations, which are often used in clinical practice. A focused systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. It was registered on the international prospective register of systematic reviews PROSPERO, which helped in identifying articles that met the inclusion criteria. Clinical and preclinical studies involving FcRn and the nasal delivery of biologics were screened, and the risk of bias was assessed across studies using the Oral Health Assessment Tool (OHAT). Among the 12 studies finally included in this systematic review (out of the 758 studies screened), 11 demonstrated efficient transcytosis of biologics through the nasal epithelium. Only three studies evaluated the potential toxicity of biologics’ intranasal delivery, and they all showed that it was safe. This systematic review confirmed that FcRn is expressed in the nasal airway and the olfactory epithelium, and that FcRn may play a role in IgG and/or IgG-derived molecule-transcytosis across the airway epithelium. However, additional research is needed to better characterize the pharmacokinetic and pharmacodynamic properties of biologics after their intranasal delivery.

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Localization of neonatal Fc receptor for IgG in aggregated lymphoid nodules area in abomasum of Bactrian camels (Camelus bactrianus) of different ages

Cited by 9 publications

References 54 publications

Impact of Glycosylation and Species Origin on the Uptake and Permeation of IgGs through the Nasal Airway Mucosa

Impact of Glycosylation and Species Origin on the Uptake and Permeation of IgGs through the Nasal Airway Mucosa

Bacterial community analysis on the different mucosal immune inductive sites of gastrointestinal tract in Bactrian camels

FcRn as a Transporter for Nasal Delivery of Biologics: A Systematic Review

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