Localization of heat shock protein 70 genes inside the rat major histocompatibility complex close to class III genes

Wurst, Wolfgang; Benesch, Christoph; Drabent, Birgit; Rothermel, E; Benecke, Bérnd-Joachim; Günther, Eberhard

doi:10.1007/bf02421469

Cited by 59 publications

(29 citation statements)

References 16 publications

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“…the major histocompatibility complex. 29 Genes in this complex are associated with the development of hypertension. 30 Thus, at least in the above-mentioned animal species, the increased thermosensitivity might be genetically linked with hypertension.…”

Section: Discussionmentioning

confidence: 99%

Hypertension, aging, and myocardial synthesis of heat-shock protein 72.

et al. 1994

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We determined the temperature-induced synthesis of the 72-kD heat-shock protein (hsp72) in hearts of normotensive and spontaneously hypertensive rats (SHR) subjected to whole-body hyperthermia (42.0±0.5°C for 15 minutes). The animals were studied at three diiferent ages: young (2 months), adult (6 months), and old (18 months). The hsp72 was determined by Western blot analysis using a monoclonal antibody. The results were calculated densito-metrically as a percentage of a commercial standard. Young SHR responded to hyperthermic stress with increased synthesis of hsp72 compared with age-matched normotensive rats (298.8 ±70.0% versus 88.3±25.5%). This trend was maintained in adult rats (118.1±31.0% A common feature of genetically hypertensive animals is a greater sensitivity to environmental temperature with respect to that of their normotensive control counterparts. 1-2 The increased thermosensitivity is genetically linked with hypertension 3 and at the molecular level is characterized by an over-expression of heat-shock proteins, 46 a group of highly conserved proteins synthesized during stress and involved in cellular protection. 712 Increased synthesis of the 72-kD heat-shock protein (hsp72) is a specific marker of the stress response in the heart as hsp72 is induced in the myocardium after heat treatment, ischemia, hemodynamic overload, or hypox-j a i3-i6 T n e synthesis of hsp72 in the myocardium of heat-shocked hypertensive animals has never been fully studied. This is particularly relevant because recently the induction of hsp72 synthesis has been shown to protect the myocardium against ischemia, 9 " 11 a pathological condition often associated with hypertension. During aging, the heart undergoes progressive functional alterations and becomes more vulnerable to isch-emic insult. 17 This is true of the hypertrophic heart of the spontaneously hypertensive rat (SHR). 18-19 Several studies show an age-dependent decrease in the expression of heat-shock protein in response to environmental stress in both isolated cell systems 20-21 and tissues. 22-23 The first aim of this study was to determine the synthesis of hsp72 in the heart of normotensive Wistar-Kyoto (WKY) rats and SHR subjected to whole-body hyperthermia. The second aim was to investigate the effects of aging on the synthesis of hsp72. versus 54.8±21.3%) but not in old rats (65.3±29.4% versus 43.6±15.1%). Aging caused a reduction of hsp72 expression in response to hyperthermic stress in both SHR (4.6-fold) and normo-tensive rats (twofold). These data show that hearts of young and adult SHR respond to heat shock with enhanced synthesis of hsp72. This abnormal response, attenuated by aging, is independent of the presence and degree of hypertension or hypertrophy and is potentially linked to the genetic determination of the disease. (Hypertension. 1994^4:620-624.) Key Words • heat-shock proteins • hypertension, essential • aging • heart Methods Animals The experiments were carried out in compliance with the Guide for the Care and Use of Laboratory Animals o...

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Section: Discussionmentioning

confidence: 99%

Hypertension, aging, and myocardial synthesis of heat-shock protein 72.

et al. 1994

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“…Some members of the HspTO gene family have been mapped to the major histocompatibility complex (Mhc), which controls immune responsiveness. Linkage between Mhc and HspTO genes has been shown in human (Sargent et al 1989), rat (Wurst et al 1989), mouse (Gaskins et al 1990;Jacob and Hwang 1992;Hunt et al 1993;Snoek et al 1993), goat (Cameron et al 1990), cattle (Grosz et al 1992), pig (Nunes et al 1993), and frog (Salter-Cid et al 1994). In most of these species three Hsp70 genes have been detected in the Mhc.…”

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confidence: 92%

Comparative analysis of the three major histocompatibility complex-linked heat shock protein 70 (Hsp70) genes of the rat

Walter¹,

Rauh²,

Günther³

1994

Immunogenetics

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The organization of the three major histocompatibility complex (Mhc)-linked heat shock protein 70 (Hsp70) genes Hsp70-1, Hsp70-2, and Hsp70-3, and the nucleotide sequences of these genes, are presented for the rat. Hsp70-1 and Hsp70-2 gene products are identical at the amino acid level. From the pattern of sequence similarity of the orthologous Mhc-linked Hsp70 genes of rat, human, and mouse, it is concluded that the gene duplications leading to the three-gene cluster occurred before the separation of the primate and rodent lines and that the Hsp70-1 and Hsp70-2 genes of rat and human might have undergone homogenization of their sequences.

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“…n. [13][14][15] At least two copies of hsp70 were localized in the major histocompatibility complex of humans, 16 mice, 17 and rats. 18 The area in which hsp70 is localized is of interest since it contains other genes potentially relevant to environmental interactions, such as 21-hydroxylase (21-OH) and TNFa. To evaluate whether hsp70, which is increasingly expressed in hypertensive cells, segregates with blood pressure, we have searched for polymorphisms in various hypertensive and normotensive rat strains and studied segregation with hypertension in recombinant inbred (RI) and congenic strains.…”

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confidence: 99%

Restriction fragment length polymorphism of hsp70 gene, localized in the RT1 complex, is associated with hypertension in spontaneously hypertensive rats.

et al. 1992

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Previous studies from our laboratory have demonstrated that the intermediate phenotype of thermosensitivity is present in hypertensive mice and rats. Increased expression of hsp70 caused by increased transcription rate was demonstrated in vivo, in organs, and in cultured cells from spontaneously hypertensive rats and hypertensive mice. In this study, a polymorphism of this gene was revealed with BamHl enzyme by using a human hsp70 probe. A 4.4-kb fragment was visualized in normotensive rats (Brown-Norway BN.Ix and Sprague-Dawley), and a 3.0-kb fragment was found in spontaneously hypertensive rats (SHR) of three different origins and in Wistar and Buffalo rats. Both fragments were present in the Wistar-Kyoto rat strain. The present study mapped the polymorphism of hsp70 into the RT1 complex in BN.1K and SHR.1N congenic strains. The hsp70 restriction fragment length polymorphism is associated with a blood pressure difference of IS mm Hg in recombinant inbred strains. These results justify the search for a mechanism by which hsp70 could influence blood pressure. (Hypertension 1992;19:611-614) KEYWORDS • restriction fragment length polymorphisms spontaneously hypertensive rats heat-shock proteins • DNA, recombinant

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Localization of heat shock protein 70 genes inside the rat major histocompatibility complex close to class III genes

Cited by 59 publications

References 16 publications

Hypertension, aging, and myocardial synthesis of heat-shock protein 72.

Hypertension, aging, and myocardial synthesis of heat-shock protein 72.

Comparative analysis of the three major histocompatibility complex-linked heat shock protein 70 (Hsp70) genes of the rat

Restriction fragment length polymorphism of hsp70 gene, localized in the RT1 complex, is associated with hypertension in spontaneously hypertensive rats.

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