Restriction fragment length polymorphism of hsp70 gene, localized in the RT1 complex, is associated with hypertension in spontaneously hypertensive rats.

Hamet, Pavel; Kong, Dewen; Pravenec, Michal; Kuneš, Jaroslav; Křen, Vladimı́r; Klír, P; Sun, Yulin; Tremblay, Johanne

doi:10.1161/01.hyp.19.6.611.a

Cited by 59 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A BP quantitative trait locus (QTL) was identified within the differential chromosomal segment of SHR.1N in a set of recombinant inbred strains derived from the same progenitors (SHR and BN.Lx/Cub) as the congenic strain studied here. 29,43,44 In addition, the existence of the QTL was confirmed by generating a congenic strain. 29 A BP QTL within the same region of rat chromosome 20 was also identified in a cross involving New Zealand genetically hypertensive and BN rats.…”

Section: Discussionmentioning

confidence: 96%

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

et al. 2003

View full text Add to dashboard Cite

Abstract-Previous linkage and association studies have suggested that a region of human chromosome 6 containing the tumor necrosis factor (TNF)-␣ gene is involved in the pathogenesis of obesity and obesity-associated hypertension. The aim of the present investigation was to establish whether a segment of rat chromosome 20 (RNO20), which also contains the TNF-␣ gene, determines diet-induced changes in adiposity and blood pressure (BP). The results showed that a transfer of the RNO20 segment from the normotensive Brown Norway (BN) rat onto the background of the spontaneously hypertensive rat (SHR) is associated with a significantly greater increase in adiposity, glucose intolerance, circulating leptin levels, and BP during 12-week, high-fat-diet feeding. In contrast, the transfer is not associated with significant changes in these variables during 12-week, normal-diet feeding. In addition, sequencing of the TNF-␣ gene revealed differences between SHR and BN in the 5Ј-and 3Ј-regulatory regions of the gene. Subsequent analyses of TNF-␣ gene expression in fat, muscle, and liver, however, did not provide support for the functional involvement of these differences. In summary, the investigated RNO20 segment contains 1 or more gene variants that affect adiposity, glucose tolerance, serum leptin levels, and BP, but only when the animals are exposed to a particular environment, ie, high-fat-diet feeding. Further studies are needed to identify genes mediating these effects. Considering current changes in our lifestyle involving an increased calorie and fat intake, we believe that gene-environment interactions, such as those described here, play an important role in the current epidemic of obesity and obesityassociated hypertension.

show abstract

Section: Discussionmentioning

confidence: 96%

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Raised BP has direct effects on the vasculature, 24,25 and vessels subjected to greater mechanical and shear stress express heat shock proteins and are more prone to the development of atherosclerosis. 20,22,25,26 In addition, Hsp70 mRNA levels are enhanced in hypertensive animals, 27,28 and there is a greater accumulation of heat stress-induced Hsp70 mRNA in peripheral blood lymphocytes from hypertensive humans compared with that in NT control subjects. 29 Although these findings demonstrate a stress response to hypertension, a definitive causative link between heat shock protein expression/release and the development of vascular disease has yet to be established.…”

Section: Discussionmentioning

confidence: 99%

Cardiovascular disease

2011

Oxford Handbook of Anaesthesia

View full text Add to dashboard Cite

Abstract-The phylogenetically conserved nature of heat shock proteins (Hsp) has led to the proposition that they may provide a link between infection and the inflammatory component to vascular disease. Hypertension is associated with atherosclerosis. Here, we measured circulating heat shock protein and heat shock protein antibody levels in association with borderline hypertension. Seventy-two men with borderline hypertension patients and 75 normotensive control subjects (diastolic blood pressure 85 to 94 and Ͻ80 mm Hg, respectively) were selected from a population-screening program. The levels of Hsp60; Hsp70; and anti-human Hsp60, anti-human Hsp70, and anti-mycobacterial Hsp65 antibodies were determined with enzyme immunoassay. The presence of carotid atherosclerosis and the intima-media thickness values were determined with ultrasonography. A major novel observation in this report was the detection of circulating Hsp60, which was present at a significantly enhanced level in patients with borderline hypertension. Furthermore, serum Hsp60 was associated with intima-media thicknesses (PϽ0.01). Anti-Hsp65 antibody levels were higher in borderline hypertension (PϽ0.001), whereas Hsp70 and anti-Hsp70 antibody levels did not differ. In contrast to anti-Hsp65 antibody, anti-Hsp60 antibody levels were lower in borderline hypertension (PϽ0.03), although the difference was quantitatively small. None of the parameters evaluated were associated with atherosclerosis, metabolic factors, or smoking. We identified elevated Hsp60 levels in patients with borderline hypertension and an association between early atherosclerosis and Hsp60 levels. The physiological role of Hsp60 release has yet to be defined, but given the proinflammatory properties, these proteins could be involved in the induction/progression of both hypertension and atherosclerosis, as well as being markers for early cardiovascular disease. (Hypertension. 2000;36:303-307.)

show abstract

“…BN.lx and SHRp livers were provided by the Institute of Physiology, Czech Academy of Science, and the Biology Institute at Charles University in Prague. Genomic DNA was prepared as described elsewhere [18]. VSMC from BN.…”

Section: Methodsmentioning

confidence: 99%

“…In the past few years, we have focused on the general stress response of hypertensive patients, animals and cell lines [14][15][16][17][18]. We believe that the abnormal response to various environmental stresses in hypertension is genetically-determined and that genes induced by heat or other stressors, modify the development of hypertension.…”

Section: Introductionmentioning

confidence: 99%

“…HSP27, HSPT0 and HSP104 have been found to be involved in the regulation of thermotolerance [25][26][27][28]. We have reported previously that abnormal HSP70 gene expression is evident in hypertensive subjects and animals after exposure to heat and other stressful stimuli [15,16,29] and the restriction fragment length polymorphism (RFLP) of the HSP70 locus segregates with an increment of blood pressure in 30 recombinant inbred strains (RIS) of rats [18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Candidate genes of hypertension with defective environmental expression

et al. 1995

View full text Add to dashboard Cite

Previous studies in our laboratory have demonstrated that the thermosensitivity locus cosegregates with blood pressure and that the elevated expression and restriction fragment length polymorphism of HSP70 gene are associated with hypertension. Cell protection against environmental stressors such as heat and chemicals is often accompanied by up-regulated expression of a wide spectrum of heat shock genes(HSP). To further investigate the interrelation between HSP expression and blood pressure regulation, we employed an effective method of cloning 2 potential hypertension-related HSPs. Synthetic oligonucleotides corresponding either to a highly-conserved region of the known HSP family or a repetitive sequence in the proteinencoding gene were used as target primers for polymerase chain reaction (PCR). cDNA prepared from heat-stressed and non-stressed vascular smooth muscle cells (VSMC) of Brown Norway rats (BN.lx) and spontaneously hypertensive rats (SHRp) respectively served as template in the reaction. The PCR products were subsequently analyzed in a single-stranded conformational polymorphism (SSCP) electrophoresing system. Differential gene expression in BN. lx and SHRp was seen on autoradiographs of SSCP gel by comparing the migration patterns of PCR-amplified

show abstract

Restriction fragment length polymorphism of hsp70 gene, localized in the RT1 complex, is associated with hypertension in spontaneously hypertensive rats.

Cited by 59 publications

References 22 publications

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

Segment of Rat Chromosome 20 Regulates Diet-Induced Augmentations in Adiposity, Glucose Intolerance, and Blood Pressure

Cardiovascular disease

Candidate genes of hypertension with defective environmental expression

Contact Info

Product

Resources

About