Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

Jongsma, A. P. M.; Someren, H. van; Westerveld, A.; Hagemeijer, A.; Pearson, P. L.

doi:10.1007/bf00385730

Cited by 107 publications

(22 citation statements)

References 21 publications

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“…Artificial mixtures of cell extracts demonstrated the two parental bands whereas hybrids positive for mouse ENO-1 exhibited three bands of activity: the two parental forms and a single heteropolymer. This ENO phenotype is similar to that seen in mouse X human or Chinese hamster X human hybrids and indicates that ENO is a dimeric enzyme (11)(12)(13)(14).…”

Section: Resultssupporting

confidence: 64%

“…The electrophoretic mobilities of mouse PGM and PGD and the assignment of Pgm-2 and Pgd to mouse chromosome 4 have been reported (15,16). Mouse PGM-2 is homologous to human PGM1 which has been assigned to human chromosome 1 (11,16).…”

Section: Resultsmentioning

confidence: 99%

“…In man, PGD, ENO1, PGM1 (homologous to mouse Pgm-2), and AK2 have been assigned to the short arm of human chromosome 1 (11)(12)(13)(14). Thus, a large autosomal region has apparently been conserved through the 80 X 106 years of evolution that separate mouse and man.…”

Section: Discussionmentioning

confidence: 99%

“…Determination of which linkage groups have been conserved or disrupted over a wide range of species could yield information essential to understanding the evolution of the mammalian genome. Although conservation of X-linked genes is the rule in all mammalian species examined (1)(2)(3)(4)(5) (11)(12)(13)(14). In the mouse, the genes coding for PGD and PGM-2 (homologous to human PGM1) are linked and located on mouse chromosome 4 (7,15,16 …”

mentioning

confidence: 99%

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Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p

Lalley

Francke

Minna

1978

Proc. Natl. Acad. Sci. U.S.A.

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Section: Resultssupporting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p

Lalley

Francke

Minna

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…Genetic mapping by somatic cell hybridization (3)(4)(5)(6)(7) and by the segregation of unique chromosomal rearrangements involving the MHC in families (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) has permitted localization of the serologically polymorphic 45,000-dalton glycoproteins of the class I genes (HLA-A, -B, and -C) to the short arm of human chromosome 6, with the shortest region of overlap being the 6p21 band (7,17). Associated with the polymorphic glycoproteins of the HLA-A, -B, and -C antigens is a nonglycosylated 12,000-dalton invariant chain, 82-microglobulin, that has been assigned to human chromosome 15 (20,21).…”

mentioning

confidence: 99%

Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

Kirsch¹,

Nance²,

Evans³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization.Our data indicate that a cloned genomic probe cross-hybridizing to HLA-A, -B, and -C heavy chain loci is homologous to sequences located on chromosome 6 at band p21.3 while a subclone of the genomic HLA-DR a-chain gene corresponding to the nonpolymorphic p34 protein is homologous to sequences in band 6p21.1. Our data suggest that this technique may permit the estimation of map distances between linked gene loci, assuming a uniform frequency of map units in the human genome. The relative positions of these genes was confirmed in a mother and son carrying a chromosome rearrangement involving 6p and 14p in which the sequences hybridizing to a DR a-chain genomic clone were found at the distal end of the 6p-chromosome [der(6)] while the sequences hybridizing to the HLA-A, -B, -C a-chain probe were found in the 14p+ chromosome [der(14)].The major histocompatibility complex (MHC) in humans is a cluster of at least three gene families encoding cell surface glycoproteins-i.e., genes for class I (HLA-A, -B, and -C) and class II (HLA-DR) antigens in addition to class III genes (complement factors)-and spans 2-3 x 103 kilobase pairs (kb) of DNA (1, 2). Genetic mapping by somatic cell hybridization (3-7) and by the segregation of unique chromosomal rearrangements involving the MHC in families (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) has permitted localization of the serologically polymorphic 45,000-dalton glycoproteins of the class I genes (HLA-A, -B, and -C) to the short arm of human chromosome 6, with the shortest region of overlap being the 6p21 band (7,17). Associated with the polymorphic glycoproteins of the HLA-A, -B, and -C antigens is a nonglycosylated 12,000-dalton invariant chain, 82-microglobulin, that has been assigned to human chromosome 15 (20,21). The class II genes, or the immune response genes, consist of heterodimers composed of a 34,000-dalton (HLA-DR a) glycoprotein for which no electrophoretic polymorphisms have been demonstrated and a polymorphic 29,000-dalton (HLA-DR (3) glycopeptide (21)(22)(23)(24)(25). Polymorphism of the HLA-DR , gene has permitted a regional assignment of this locus to chromosome 6 proximal to 6p22.4 by a family study (15).Recently, the HLA-DR a-chain locus has been mapped by using somatic cell hybrids (26) and, furthermore, this assignment has been refined to the region 6p2105-6p23 (27) by study of genomic blotting patterns in cell lines containing individual chromosomal deletions of the short arm of chromosome 6. Data on recombination within the HLA complex and with closely linked markers such as glyoxylase 1 (GLO 1) have permitted inferences to be drawn about the order and map distances between marker loci on the proximal portion of the short arm of chromosome 6, as follows: GLO 1-4 centimorgans (cM)-HLA-DR-0.3 cM-BF, C4B, C4A, C2-0.7 cM-HLA-B-0.1 cM-HLA-C-0.7 cM-HLA-A (16-18).Improvements in chromosomal i...

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Gene Clusters and the HLA System

Bodmer

1979

Ciba Foundation Symposium 66 ‐ Human Genetics: Possibilities and Realities

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Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

Cited by 107 publications

References 21 publications

Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p

Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p

Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

Gene Clusters and the HLA System

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