Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs

Sandbrink, J.M.; Ooijen, J.W. van; Purimahua, C. C.; Vrielink, M.; Verkerk, Ruud; Zabel, P.; Lindhout, P.

doi:10.1007/bf00221988

Cited by 61 publications

(30 citation statements)

References 21 publications

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“…A possible mechanism suggested is that there may be a gene (or chromosomal rearrangement) present in the distorted segregation region that affects gametophytic or zygotic competitiveness [34]. For a correctly inferred marker order and map distance, influence of segregation distortion on QTL analysis should be negligible [54]. The detection of QTLs through composite interval mapping which involves stepwise regression does not get affected by segregation distortion of marker loci [55].…”

Section: Genetic Linkage Map Constructionmentioning

confidence: 99%

Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum)

Vengadessan¹,

Rai

Bapu

et al. 2013

ISRN Genetics

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A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F 2:3 mapping population progenies produced from a cross between two pearl millet inbred lines having diverse parentage. The skeleton linkage map covered 1019 cM and it comprised of 44 markers distributed across the seven linkage groups. Average adjacent-marker intervals ranged from 14 cM on LG1 to 38 cM on LG6, with an overall mean of 23 cM. Using the F 2 linkage map and phenotypic data from the F 2 and F 2:3 generations of the mapping population, a total of 18 putative QTLs were detected for the three sink-size components. Eight QTLs explained 42.7% of observed phenotypic variation for panicle length using the F 2:3 data set. For panicle diameter, 5 QTLs explained 45.8% of observed phenotypic variation. Similarly for grain size, 5 QTLs explained 29.6% of phenotypic variation. Genomic regions associated with panicle length, panicle diameter, and grain size were comapped on LG6 between Xpsms88 and Xpsms2270, indicating the existence of a gene or gene cluster. The QTLs for panicle length on LG2 and LG6 (LOD > 3 in both F 2 and F 2:3 data sets), for panicle diameter on LG2 and LG3 (LOD > 14 in the F 2:3 data set), and for grain size on LG3 and LG6 (LOD > 3 in both F 2 and F 2:3 data sets) were identified as promising candidates for validation prior to possible application in marker-assisted breeding.

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Section: Genetic Linkage Map Constructionmentioning

confidence: 99%

Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum)

Vengadessan¹,

Rai

Bapu

et al. 2013

ISRN Genetics

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“…In glasshouse tests, the average lesion size (LS) was only 1.4 mm 2 compared to 23.0-108.0 mm 2 on susceptible tomato accessions. S. arcanum LA2157 is known as resistance source to other pathogens, including bacterial canker (Sandbrink et al 1995;Van Heusden et al 1999), and root knot nematode (Veremis et al 1999). The cross with Solanum lycopersicum (syn.…”

Section: Introductionmentioning

confidence: 99%

QTL identification for early blight resistance (Alternaria solani) in a Solanum lycopersicum × S. arcanum cross

Chaerani

Smulders²,

Linden³

et al. 2006

Theor Appl Genet

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Alternaria solani (Ellis and Martin) Sorauer, the causal agent of early blight (EB) disease, infects aerial parts of tomato at both seedling and adult plant stages. Resistant cultivars would facilitate a sustainable EB management. EB resistance is a quantitatively expressed character, a fact that has hampered effective breeding. In order to identify and estimate the effect of genes conditioning resistance to EB, a quantitative trait loci (QTL) mapping study was performed in F2 and F3 populations derived from the cross between the susceptible Solanum lycopersicum (syn. Lycopersicon esculentum) cv. 'Solentos' and the resistant Solanum arcanum (syn. Lycopersicon peruvianum) LA2157 and genotyped with AFLP, microsatellite and SNP markers. Two evaluation criteria of resistance were used: measurements of EB lesion growth on the F2 plants in glasshouse tests and visual ratings of EB severity on foliage of the F3 lines in a field test. A total of six QTL regions were mapped on chromosomes 1, 2, 5-7, and 9 with LOD scores ranging from 3.4 to 17.5. Three EB QTL also confer resistance to stem lesions in the field, which has not been reported before. All QTL displayed significant additive gene action; in some cases a dominance effect was found. Additive · additive epistatic interactions were detected between one pair of QTL. For two QTL, the susceptible parent contributed resistance alleles to both EB and stem lesion resistance. Three of the QTL showed an effect in all tests despite methodological and environmental differences.

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“…The use of molecular markers can facilitate tomato breeding by means of markerassisted selection (MAS) to improve agronomically important traits such as yield, fruit quality and disease resistance. In the last decade, molecular markers such as RFLP (Van Ooijen et al 1994;Sandbrink et al 1995;Stevens et al 1995), RAPD (Stevens et al 1995;Qian et al 2001), ISSR (Zietkiewicz et al 1994;Joshi et al 2000) and AFLP (Vos et al 1995) have been developed in tomato and other crops. However, the use of RFLP for breeding purposes is limited because it requires the use of radioactivity and is labour intensive; RAPD, ISSR and AFLP markers either identify only dominant alleles or are sensitive to PCR amplification conditions.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

2003

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The simple sequence repeat (SSR) or microsatellite marker is currently the preferred molecular marker due to its highly desirable properties. The aim of this study was to develop and characterize more SSR markers because the number of SSR markers currently available in tomato is very limited. Five hundred DNA sequences of tomato were searched for SSRs and analyzed for the design of PCR primers. Of the 158 pairs of SSR primers screened against a set of 19 diverse tomato cultivars, 129 pairs produced the expected DNA fragments in their PCR products, and 65 of them were polymorphic with the polymorphism information content (PIC) ranging from 0.09 to 0.67. Among the polymorphic loci, 2-6 SSR alleles were detected for each locus with an average of 2.7 alleles per locus; 49.2% of these loci had two alleles and 33.8% had three alleles. The vast majority (93.8%) of the microsatellite loci contained di- or tri-nucleotide repeats and only 6.2% had tetra- and penta-nucleotide repeats. It was also found that TA/AT was the most frequent type of repeat, and the polymorphism information content (PIC) was positively correlated with the number of repeats. The set of 19 tomato cultivars were clustered based on the banding patterns generated by the 65 polymorphic SSR loci. Since the markers developed in this study are primarily from expressed sequences, they can be used not only for molecular mapping, cultivar identification and marker-assisted selection, but for identifying gene-trait relations in tomato.

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Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs

Cited by 61 publications

References 21 publications

Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum)

Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum)

QTL identification for early blight resistance (Alternaria solani) in a Solanum lycopersicum × S. arcanum cross

Development and characterization of simple sequence repeat (SSR) markers and their use in determining relationships among Lycopersicon esculentum cultivars

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