Localization of genes encoding metallothionein-like protein ( mt2 and smtb ) in the brain of zebrafish

Teoh, Seong Lin; Ogawa, Satoshi; Parhar, Ishwar S.

doi:10.1016/j.jchemneu.2015.10.004

Cited by 9 publications

(4 citation statements)

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“…The fish were fed adult zebrafish food twice daily. Experiments were conducted after 2 weeks of acclimatizing to laboratory conditions (Howe et al, 2013;Teoh et al, 2015). The sample size was calculated using Power and Sample size calculation (PPS) version 3.1.6, October 2018 by William D. Dupont and Walton D Plummer, Jr. with α score of 0.05, power of 0.8, and a m score of 1, based on the study conducted on the assessment of zebrafish as a PD model (Sarath Babu et al, 2016;Vijayanathan et al, 2017).…”

Section: The Animalsmentioning

confidence: 99%

Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Omar,

Kumar,

Teoh

2023

Front. Neurosci.

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IntroductionParkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish.MethodsFish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection.ResultsBetween one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment.DiscussionOver a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies.

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Section: The Animalsmentioning

confidence: 99%

Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Omar,

Kumar,

Teoh

2023

Front. Neurosci.

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“…Two metallothionein homologs, metallothionein-2 (mt2) and metallothionein-B-like (mtbl), represent the predominant inducible forms of metallothionein present in larval zebrafish (Wu et al, 2008; Teoh et al, 2015; Roesijadi, 1992; Andrews, 1990, 2000; Kagi, 1991; Palmiter, 1998). In addition to the antioxidant genes investigated, we hypothesized that increased expression of metallothionein mRNA, might be an underlying mechanism of the protective effect of low levels of Zn and Cd induced olfactory toxicity.…”

Section: Discussionmentioning

confidence: 99%

Comparative effects of cadmium, zinc, arsenic and chromium on olfactory-mediated neurobehavior and gene expression in larval zebrafish (Danio rerio)

2018

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Studies have shown that olfactory-mediated behaviors that are critical to survival can be disrupted by exposure to certain metals. Aquatic Superfund sites often contain elevated levels of various metals, yet few have been characterized for their potential to cause olfactory toxicity. A larval zebrafish behavioral assay was developed to characterize concentration-response curves for zinc (Zn), hexavalent chromium (Cr), and arsenate (As) olfaction inhibition. Cadmium (Cd), an established olfactory toxicant, was used as a positive control. As expected, following a 24-hour exposure to Cd, we observed a reduced response to taurocholic acid (TCA), a behaviourally-active odorant, thus validating the behavioral assay. Zn exposure also decreased TCA response (IC50: 34.9 µg/L and 69.0 µg/L respectively). Behavioral responses to another odorant, L-cysteine, revealed that Zn was potentially of specific toxicity to ciliated olfactory sensory neurons.No significant changes in olfactory responses were observed from Cr and As exposures, even at exposures far greater than observed environmental concentrations.Exposures to binary mixtures of Cd and Zn indicated that Zn had a protective effect against Cd toxicity at low concentrations, but this effect disappeared at higher Zn concentrations. Quantitative analysis of whole larvae gene expression of 8 genes 4 important in mitigating metal induced oxidative stress, confirmed an antioxidant response was evoked by Cd, but not for the other metals, suggesting that oxidative stress was not a primary mechanism of Zn induced olfactory dysfunction. In summary, our study identified that Zn inhibits olfaction at environmentally-relevant concentrations, and that it may reduce Cd when present in mixtures. Our study extends the utility of zebrafish as a model species to assess olfactory toxicity in fish.5 Abbreviations: Nrf2: nuclear factor, erythroid 2-like 2, omp: olfactory marker protein b, dpf: days post fertilization, EM: E3 embryo medium, SRP: superfund research project, Zn: zinc, As: arsenic, Cr: chromium, Cd: cadmium, TCA: taurocholic acid, OE: olfactory epithelium, OSN: olfactory sensory neuron, OR: olfactory receptor, MOR: major olfactory receptor, TRPC2: Transient Receptor Potential Cation Channel Subfamily C Member 2, OB: olfactory bulb, hmox1a: heme oxygenase 1a, gstp: glutathione s-transferase pi, gclc:glutamate-cysteine ligase, catalytic subunit, nqo1: NAD(P)H dehydrogenase, quinone 1, prdx1: peroxiredoxin 1, gpx1a: glutathione peroxidase 1a, sod1: superoxide dismutase 1, soluble, sod2: superoxide dismutase 2, mitochondrial, hsp70: heat shock cognate 70-kd protein, tandem duplicate 3, actb1: actin, beta 1, gapdh: glyceraldehyde-3-phosphate dehydrogenase, hprt1: hypoxanthine phosphoribosyltransferase 1, gadd45bb: growth arrest and DNA-damage-inducible, beta b

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“…4D), where GFP-positive cells resembling neurons were adjacent to (but not co-located with) many smaller (subcellular) TSQ-Zn bodies. Drawing on a study reporting that (under normal conditions) zebrafish mt2 expression in the brain is localised to neurons and not glial cells (Teoh et al, 2015), these observations suggest that distinct neural cell populations respond to disrupted zinc homeostasis in different ways, with neurons increasing MT activity and others developing zinc inclusions.…”

Section: Metallothionein Levels Are Up-regulated In Slc30a8 Hu1798 Lamentioning

confidence: 91%

Zinc dysregulation inslc30a8(znt8) mutant zebrafish leads to blindness and disrupts bone mineralisation

Mackay

Molero

Tirathdas

et al. 2020

Preprint

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Zinc is an essential cofactor for many cellular processes including gene transcription, insulin secretion and retinal function. Excessive free Zn2+ is highly toxic and consequently intracellular zinc is tightly controlled by a system of transporters, metallothioneins (MTs) and storage vesicles. Here we describe the developmental consequences of a missense allele of zinc efflux transporter slc30a8 (znt8) in zebrafish. Homozygous slc30a8hu1798 larvae are virtually blind and develop very little or no bone mineral. We show that zinc is stored in pigmented cells (melanophores) of healthy larvae but in slc30a8hu1798 mutants it instead accumulates in the bone and brain. Supporting a role for pigment cells in zinc homeostasis, nacre zebrafish, which lack melanophores, also show disrupted zinc homeostasis. The photoreceptors of slc30a8hu1798 fish are severely depleted while those of nacre fish are enriched with zinc. We propose that developing zebrafish utilise pigmented cells as a zinc storage organ, and that Slc30a8 is required for transport of zinc into these cells and into photoreceptors.

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Localization of genes encoding metallothionein-like protein ( mt2 and smtb ) in the brain of zebrafish

Cited by 9 publications

References 37 publications

Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Comparative effects of cadmium, zinc, arsenic and chromium on olfactory-mediated neurobehavior and gene expression in larval zebrafish (Danio rerio)

Zinc dysregulation inslc30a8(znt8) mutant zebrafish leads to blindness and disrupts bone mineralisation

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