Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Omar, Noor Azzizah; Kumar, Jaya; Teoh, Seong Lin

doi:10.3389/fnins.2023.1236049

Cited by 8 publications

(6 citation statements)

References 96 publications

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“…In normal unthreatened conditions, higher mRNA expression of th2 was linked to bolder behaviour, while higher th, th2, and neuroD2 (both genes) mRNA expression was linked to higher feeding. Similar results were found for zebrafish, Danio rerio, and rainbow trout, Oncorhynchus mykiss, even though a correlation between activity and th mRNA expression was not directly assessed (Höglund et al 2017;Omar et al 2023). In zebrafish, individuals that moved shorter distance also presented lower th mRNA expression in the brain (Omar et al 2023).…”

Section: Discussionsupporting

confidence: 72%

“…Similar results were found for zebrafish, Danio rerio, and rainbow trout, Oncorhynchus mykiss, even though a correlation between activity and th mRNA expression was not directly assessed (Höglund et al 2017;Omar et al 2023). In zebrafish, individuals that moved shorter distance also presented lower th mRNA expression in the brain (Omar et al 2023). Rainbow trout individuals with high activity and feeding motivation (referred as proactive) presented higher dopamine signalling (Höglund et al 2017), which is related to high th expression (Robinson et al 2016).…”

Section: Discussionsupporting

confidence: 64%

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Size-dependent harvest mortality indirectly affects boldness, feeding rate, and behaviour-linked gene expression in a decade-long selection experiment on guppies

Diaz Pauli,

Tronci,

Gomes

et al. 2023

Preprint

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Fisheries-induced mortality is size-selective, commonly targeting large individuals, which leads to evolution towards smaller size and early maturation. However, little is known on whether behaviour is affected. Here we aimed at testing whether size-dependent harvest indirectly affects behavioural traits that might have ecological consequences. Specifically, we assessed feeding rate – which affects prey abundance –, boldness – which determines a fish vulnerability to predators –, and sociability – which determines how a fish interacts with conspecifics and ultimately affects foraging and predation avoidance. In addition, we tested whether the differences in behaviour were associated to differences in selected key genes expression, to understand its molecular regulation. With a decade-long selection experiment on guppiesPoecilia reticulata, we created populations with life histories adapted to positively size-dependent harvest, i.e., like that induced by fishing (fast life history). For comparison, we also created populations adapted to the opposite size-selection, and populations experiencing no size-selection. Fish exposed to positively size-dependent harvest were bolder, more likely to feed, were more social/aggressive, and expressed less brainavt (arginine vasotocin) relative to those exposed to negatively size-dependent harvest. In addition, higher expression ofthandth2(tyrosine hydroxylase 1 and 2), andneuroD2(neuronal differentiation factor 2) were linked with bolder behaviour and higher feeding in normal (no-threat) conditions, while higheravt,th, andneuroD2were associated with higher sociability/aggression after a threat. Fish exposed to positively size-dependent harvest presented behaviours linked to faster life histories as theoretically expected. Therefore, harvest selection does not only affect fish size and life history, but indirectly leads to boldness and higher feeding rates, which potentially results in higher vulnerability to predators and higher pressure on prey abundance, respectively. Our results suggest that size-dependent mortality have further consequences to the ecosystem, beyond the target species.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionsupporting

confidence: 64%

Size-dependent harvest mortality indirectly affects boldness, feeding rate, and behaviour-linked gene expression in a decade-long selection experiment on guppies

Diaz Pauli,

Tronci,

Gomes

et al. 2023

Preprint

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“…Since this is a time-sensitive procedure, all the steps have to be completed within 30–40 s after anesthetizing the fish. Transfer the fish to a beaker that holds three to four liters of water so that the fish can recover from the cold-water anesthesia [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Benincasa hispida Alleviates Stress and Anxiety in a Zebrafish (Danio rerio) Model

Lakshmanagowda,

Sagar,

Puttasiddaiah

et al. 2024

Life

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The Ayurvedic medical system uses fruits of the Benincasa hispida plant to treat mental diseases, including schizophrenia. The goal of the current study was to assess the aqueous extract of B. hispida fruit’s ability to relieve stress and anxiety induced in zebrafish models using neuropharmacological evaluation, which included determining behavioral parameters in tests such as the T-maze, open tank test (OTT), and light–dark preference test (LDPT). After measuring the zebrafish survival rate for 96 h, the LC50 was found to be 5 µg. AChE (acetylcholinesterase) inhibitory activity and the status of antioxidant enzymes (SOD, CAT, and LDH) were also used to evaluate the toxicity. Furthermore, the administration of the aqueous extract of B. hispida fruit increased the frequency of entry and duration of time spent in the bright section, suggesting a noteworthy reduction in levels of stress and anxiety. Additionally, the antistress and antianxiety activity was confirmed by the docking studies’ mechanism of action, which involves the AChE receptor binding stability of the homogalactaconan molecule found in the aqueous extract of B. hispida fruit. Overall, the findings of this study demonstrated that the aqueous extract of B. hispida fruit is a viable therapeutic molecule for the creation of novel drugs and the treatment of stress since it has the therapeutic advantage of reversing the negative effects of stress and anxiety.

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“…The PD model was developed according to the protocol described by Omar et al (2023) . Briefly, a single dose of 100 μg/g of body weight (bw) per fish of MPTP (Cat#M0896, Sigma-Aldrich, St Louis, MO, USA) was administered intraperitoneally using a 30G needle.…”

Section: Methodsmentioning

confidence: 99%

“…In our prior study, we observed that the most effective PD model in zebrafish involved assessments conducted on day 3 following the MPTP insult. However, it is worth noting that any assessments should be completed within a 10-day window post-insult, as beyond this timeframe, zebrafish exhibited the ability to reverse the neurotoxic effects induced ( Omar et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model

Omar,

Kumar,

Teoh

2023

Front. Pharmacol.

Self Cite

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Introduction: Neurotrophin-3 (NT3) is a neuroprotective growth factor that induces the development, maintenance and survival of neurons. This study aims to localize NT3-expressing cells in the adult zebrafish brain and examine the role of NT3 in a zebrafish Parkinson’s disease (PD) model.Methods: Cellular localization of NT3 in adult zebrafish brains was conducted using in situ hybridization. Subsequently, adult zebrafish were injected intraperitoneally with 100 μg/g of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and treated with 400 ng/g body weight of recombinant NT3 (rNT3) via intracranial injection 24 h following MPTP injection. The fish were assessed for neurobehavioral, gene expression, immunohistology, and protein analysis on days 3, 5 and 10 post-MPTP injection.Results: Our findings showed that NT3 was extensively expressed throughout the adult zebrafish brain in neurons. Administration of rNT3 has significantly improved locomotor activity, with upregulation of th1, dat, ntf3 and bdnf gene expressions compared to MPTP-induced zebrafish. Dopaminergic neurons were also significantly increased in the zebrafish brain following rNT3 treatment. ELISA analysis reported raised GST and decreased caspase-3 levels on day 3 of assessment. The trophic changes of rNT3, however, decline as the assessment day progresses.Conclusion: This study is the first to examine the role of NT3 in the adult zebrafish PD model. NT3 has remarkable trophic effects in the zebrafish PD model. However, further study is needed to examine the dosage requirements and long-term effects of NT3 in PD.

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Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Cited by 8 publications

References 96 publications

Size-dependent harvest mortality indirectly affects boldness, feeding rate, and behaviour-linked gene expression in a decade-long selection experiment on guppies

Size-dependent harvest mortality indirectly affects boldness, feeding rate, and behaviour-linked gene expression in a decade-long selection experiment on guppies

Benincasa hispida Alleviates Stress and Anxiety in a Zebrafish (Danio rerio) Model

Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model

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