Localization of four new markers to pig chromosomes 1, 6, and 14 by radioactive in situ hybridization

Mellink, C.; Lahbib-Mansais, Y.; Yerle, Martine M.; Gellin, Joël

doi:10.1159/000133588

Cited by 18 publications

(8 citation statements)

References 11 publications

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“…Three other genes in this interval have previously been mapped in the pig. Insulin-like growth factor-1 (IGF1) and tumor rejection antigen 1 (TRA1) both map to SSC5 (Archibald et al, 1995;Hawken et al, 1999;Lahbib-Mansais et al, 2000), while d-amino acid oxidase (DAO) maps to SSC14 (Mellink et al, 1993;Lahbib-Mansais et al, 2000). Our results suggest that STAB2 and SART3 flank the HSA12 breakpoint and the distance between these genes in the human genome is predicted to be less than 5 Mbp (UCSC April 2003 assembly and NCBI Build 33; Table 2).…”

Section: Resultsmentioning

confidence: 88%

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Farber

Raney

Rilington

et al. 2003

Cytogenet Genome Res

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Genes located on human chromosome 12 (HSA12) are conserved on pig chromosomes 5 and 14 (SSC5 and SSC14), with HSA12q23.3→q24.11 harboring the evolutionary breakpoint between these chromosomes. For this study, pig sequence-tagged sites (STS) were developed for nine HSA12 genes flanking this breakpoint. Radiation hybrid (RH) mapping using the IMpRH panel revealed that COL2A1, DUSP6, KITLG, PAH and STAB2 map to SSC5, while PXN, PLA2G1B, SART3 and TCF1 map to SSC14. Polymorphisms identified in COL2A1, DUSP6, PAH, PLA2G1B and TCF1 were used for genetic linkage mapping and confirmed the map locations for these genes. Our results indicate that the HSA12 evolutionary breakpoint occurs between STAB2 and SART3 in a region spanning less than five million basepairs. These results refine the comparative map of the HSA12 evolutionary breakpoint region and help to further elucidate the extensive gene order rearrangements between HSA12 and SSC5 and 14.

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Section: Resultsmentioning

confidence: 88%

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Farber

Raney

Rilington

et al. 2003

Cytogenet Genome Res

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“…Furthermore, by referring to the range numbers of its neighbor genes and the known exact locations of DAO1 and GGT1 demonstrated by previous studies (Mellink et al, 1993;Zambonelli et al, 1998), porcine SELM was computationally mapped to 14q21. These porcine genes were organized similarly to their homologues at human 22q12.2 (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Molecular cloning, chromosomal localization and expression profiling of porcine selenoprotein M gene

et al. 2011

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Selenoprotein M may regulate a myriad of biological processes through its redox function. In pigs, neither the nucleotide sequence nor the amino acid sequence is known. Furthermore, patterns of tissue expression and regulation by dietary selenium (Se) have not been examined. We determined the full coding sequence (CDS) and the chromosomal location of the porcine gene, SELM, and described its expression profile in vivo under different dietary Se concentrations. The cDNA sequence of porcine SELM from the start codon to the poly(A) tail was cloned by reverse transcription PCR. The CDS contained 429 bases with a typical mammalian selenocysteine insertion sequence of form 2 (F2) located in the 3′-untranslated region. The gene was mapped to chromosome 14q21, where porcine SELM and its neighboring genes exhibited a similar organization to human homologues on chromosome 22q12.2. The expression pattern of SELM mRNA in muscle, thyroid, cerebral cortex, pituitary, testis, liver, and kidney was analyzed with real-time quantitative PCR in young male pigs fed a Se-deficient corn-soybean meal basal diet supplemented with 0.0, 0.3, or 3.0 mg Se/kg in the form of Se-rich yeast. Though the SELM mRNA abundance in each of the 7 tissues was not affected by the dietary Se concentrations, it was significantly higher in thyroid (P < 0.01) than in cerebral cortex, pituitary, testis, liver, and kidney at all of the 3 dietary Se concentrations.

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“…FABP3 gene has been shown to be associated with IMF content (Lee et al 2010). Hormone-sensitive lipase gene (LIPE), which maps to chromosome 6 (Mellink et al 1993), has been involved in the free fatty acids mobilization (Miyoshi et al 2008;Zechner et al 2009). Lipoprotein lipase gene (LPL) maps to chromosome 14 (Gu et al 1992) and is responsible for lipid deposition and mobilization (Luo et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

Xue¹,

Wang²,

Bang-quan³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The aim of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in candidate genes with fatness traits in the Longissimus dorsi muscle of pigs. The polymorphisms of genes were investigated, which included beta-3-adrenergic receptor gene (ADRB3), heart fatty acid-binding protein gene (FABP3), and hormone-sensitive lipase gene (LIPE) as well as lipoprotein lipase gene (LPL). The intramuscular fat (IMF) content and fatty acid composition contents in Longissimus dorsi muscle samples were measured. Results showed that ADRB3, LIPE, and LPL SNPs were associated with IMF content (P < 0.05). ADRB3 AG heterozygotes exhibited higher IMF content. LIPE A allele was associated with greater IMF content. LPL CT heterozygotes exhibited the lowest IMF content. ADRB3 c.1192G>A had highly significant association with the total monounsaturated fatty acid (MUFA) (P < 0.01) and the total polyunsaturated fatty acid (PUFA) (P < 0.01). LIPE c.442G>A was significantly associated with the contents of C12:0 and C14:0 (P < 0.05). LPL c.624C>T was significantly associated with the percentage of C16:1 (P < 0.05) and the percentage of total saturated fatty acid (SFA) (P < 0.05). The pigs with ADRB3 G allele had more MUFA, and the pigs with LPL T allele had less SFA, implying that the ADRB3 G and LPL T in pigs may be beneficial to human health. In conclusion, the results suggest that these genetic markers are important sources of the variations for the pork selection to obtain favourable meat with higher IMF levels and appropriate fatty acid composition.

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Localization of four new markers to pig chromosomes 1, 6, and 14 by radioactive in situ hybridization

Cited by 18 publications

References 11 publications

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Molecular cloning, chromosomal localization and expression profiling of porcine selenoprotein M gene

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

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