Localization of Expression of Three Cold-Induced Genes,<i>blt101, blt4.9,</i> and <i>blt14</i>, in Different Tissues of the Crown and Developing Leaves of Cold-Acclimated Cultivated Barley1

Pearce, R. S.; Houlston, Claire E.; Atherton, Kathryn M.; Rixon, Jane E.; Harrison, Paul R.; Hughes, Monica A.; Dunn, Michael

doi:10.1104/pp.117.3.787

Cited by 81 publications

(56 citation statements)

References 28 publications

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“…In addition to SOS1, the promoter activity of a hypothetical osmosensor, ATHK1, seemed to be higher in vascular bundles, especially in the roots (Urao et al, 1999). Some stressinducible and tolerance genes/proteins, such as late-embryogenesis abundant (LEA) proteins and Ca 21 -dependent protein kinase, are also expressed very highly in the vascular area (Pearce et al, 1998;Saijo et al, 2001). Furthermore, one of the ABA-inducible genes, rab16A, is detected in vascular bundles and is induced in the leaves and roots by ABA, NaCl, and desiccation treatments .…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Localization of an Abscisic Acid Biosynthetic Enzyme, AAO3, in Arabidopsis

Koiwai

Nakaminami²,

Seo³

et al. 2004

Plant Physiology

208

140

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Arabidopsis aldehyde oxidase 3 (AAO3) is an enzyme involved in abscisic acid (ABA) biosynthesis in response to drought stress. Since the enzyme catalyzes the last step of the pathway, ABA production sites may be determined by the presence of AAO3. Here, AAO3 localization was investigated using AAO3 promoter:AAO3-GFP transgenic plants and by an immunohistochemical technique. AAO3-GFP protein exhibited an activity to produce ABA from abscisic aldehyde, and the transgene restored the wilty phenotype of the aao3 mutant. GFP-fluorescence was detected in the root tips, vascular bundles of roots, hypocotyls and inflorescence stems, and along the leaf veins. Intense immunofluorescence signals were localized in phloem companion cells and xylem parenchyma cells. Faint but significant GFP-and immuno-fluorescence signals were observed in the leaf guard cells. In situ hybridization with antisense AAO3 mRNA showed AAO3 mRNA expression in the guard cells of dehydrated leaves. These results indicate that the ABA synthesized in vascular systems is transported to various target tissues and cells, and also that the guard cells themselves are able to synthesize ABA.

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Section: Discussionmentioning

confidence: 99%

Tissue-Specific Localization of an Abscisic Acid Biosynthetic Enzyme, AAO3, in Arabidopsis

Koiwai

Nakaminami²,

Seo³

et al. 2004

Plant Physiology

208

140

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“…Thus, according to Folch and Lees (1951), ZmTLA1 represents a proteolipid, a class of extremely hydrophobic proteins. Only a few plant proteolipids are known (Capel et al, 1997;Pearce et al, 1998), and functions have not been identified for any of them. One proteolipid family highly conserved in eukaryotes (including plants) was predicted to contain two a-helical structures.…”

Section: Discussionmentioning

confidence: 99%

TRANSPARENT LEAF AREA1 Encodes a Secreted Proteolipid Required for Anther Maturation, Morphogenesis, and Differentiation during Leaf Development in Maize

et al. 2005

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We report the identification and functional analysis of TRANSPARENT LEAF AREA1 (TLA1), a maize (Zea mays) gene representing a novel class of secreted, extremely hydrophobic peptides (proteolipids) with a C-terminal Caax box-like motif. ZmTLA1 encodes 27 amino acid residues and is most strongly expressed in the egg cell and microspores. Lower transcript amounts were detected during vegetative development. Transgenic maize expressing an antisense transcript displayed a variety of phenotypes. The most visible phenotypes were dwarfism and transparent leaf areas resulting from defective morphogenesis of mesophyll, bundle sheath, stomatal, and epidermal cells during leaf development. Incomplete cell walls were observed, indicating a defect of cytokinesis. The accumulation of gerontoplasts was probably a secondary effect caused by defects of leaf cell morphogenesis. A defect of anther maturation was observed in ;30% of the plants displaying the tla phenotype. Male sterility was mainly caused by incomplete disintegration of the tapetal cell layers and tetrad callose as 90% of the microspores developed into functional pollen. Overexpression of ZmTLA1 seemed to have a lethal effect both in maize and Arabidopsis thaliana. Development of primary roots, root hairs, primary leaves, and chloroplasts was suppressed in Arabidopsis seedlings expressing an inducible ZmTLA1-green fluorescent protein (GFP) fusion protein. GFP signals were exclusively detected in cell walls. Based on our observations, we suggest that the ZmTLA1 peptide represents a class of novel plant morphogens required for the development and maturation of leaf and reproductive tissues.

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“…The regulation of coldinducible genes (RC12A, RC12B, COR6.6, COR15, COR78, DREB1, RD29A, and KIN1) during Arabidopsis development has been studied in detail, and the results indicated that the expression of these genes is regulated in different tissues both in stressed and unstressed conditions (Yamaguchi- Shinozaki and Shinozaki, 1993;Baker et al, 1994;Wang and Cutler, 1995;Xu et al, 1995;Medina et al, 2001). Lowtemperature-responsive genes (BLT101, BLT4.9, and BLT14) have been reported in barley, and these genes are also regulated during development (Pearce et al, 1998). It is possible that many genes regulated during plant development are also regulated by environmental stresses.…”

Section: Discussionmentioning

confidence: 99%

Developmentally Regulated Dual-Specificity Kinase from Peanut That Is Induced by Abiotic Stresses

Rudrabhatla

Rajasekharan

2002

Plant Physiology

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Tyrosine (Tyr) phosphorylation represents an important biochemical mechanism to regulate many cellular processes. No Tyr kinase has been cloned so far in plants. Dual-specificity kinases are reported in plants and the function of these kinases remains unknown. A 1.7-kb cDNA that encodes serine/threonine/Tyr (STY) kinase was isolated by screening peanut (Arachis hypogaea) expression library using the anti-phospho-Tyr antibody. The histidine-tagged recombinant kinase histidine-6-STY predominantly autophosphorylated on Tyr and phosphorylated the histone primarily on threonine. Genomic DNA gel-blot analysis revealed that STY kinase is a member of a small multigene family. The transcript of STY kinase is accumulated in the mid-maturation stage of seed development, suggesting a role in the signaling of storage of seed reserves. The STY kinase mRNA expression, as well as kinase activity, markedly increased in response to cold and salt treatments; however, no change in the protein level was observed, suggesting a posttranslational activation mechanism. The activation of the STY kinase is detected after 12 to 48 h of cold and salt treatments, which indicates that the kinase may not participate in the initial response to abiotic stresses, but may play a possible role in the adaptive process to adverse conditions. The transcript levels and kinase activity were unaltered with abscisic acid treatment, suggesting an abscisic acid-independent cold and salt signaling pathway. Here, we report the first identification of a non-MAP kinase cascade dual-specificity kinase involved in abiotic stress and seed development.

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Localization of Expression of Three Cold-Induced Genes,blt101, blt4.9, and blt14, in Different Tissues of the Crown and Developing Leaves of Cold-Acclimated Cultivated Barley1

Cited by 81 publications

References 28 publications

Tissue-Specific Localization of an Abscisic Acid Biosynthetic Enzyme, AAO3, in Arabidopsis

Tissue-Specific Localization of an Abscisic Acid Biosynthetic Enzyme, AAO3, in Arabidopsis

TRANSPARENT LEAF AREA1 Encodes a Secreted Proteolipid Required for Anther Maturation, Morphogenesis, and Differentiation during Leaf Development in Maize

Developmentally Regulated Dual-Specificity Kinase from Peanut That Is Induced by Abiotic Stresses

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