Localization of catecholamines in the buccal ganglia ofAplysia californica

Rathouz, Margaret; Kirk, Mark D.

doi:10.1016/0006-8993(88)90512-4

Cited by 22 publications

(27 citation statements)

References 37 publications

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“…The pattern of blue-green fluorescence observed following application of the FaGlu histological technique was similar to that described in previous reports in which a variety of histofluorescent techniques was used to visualize catecholamines (Tritt et al, 1983;Salimova et al, 1987;Rathouz and Kirk, 1988;Goldstein and Schwartz, 1989;Kabotyanski et al, 1998). On the rostral surface of each buccal hemiganglion, an ovoid cell body (Fig.…”

Section: Colocalization Of Catecholaminergic Markersmentioning

confidence: 54%

“…The two B20 cells occupy characteristic positions, and each has a distinctive bipolar morphology that results in bilateral projections to the cerebral-buccal connectives (CBCs; Rathouz and Kirk, 1988;Goldstein and Schwartz, 1989;Teyke et al, 1993;see also Figs. 1B2, 3C).…”

Section: Colocalization Of Catecholaminergic Markersmentioning

confidence: 99%

“…Ganglia were dissected, pinned out in Sylgard-lined Petri dishes, and fixed for 4 -6 hours using 4% paraformaldehyde and 0.5% glutaraldehyde (FaGlu method; Furness et al, 1977;Goldstein and Schwartz, 1989). After fixation, examination of ganglia using the filter block B-3A of a Nikon Optiphot fluorescence microscope revealed specific cells with a bluish tint characteristic of catecholaminergic neurons (Goldstein and Schwartz, 1989; see also various methods based on glyoxylic acid and aldehyde-induced histofluorescence reported by Tritt et al, 1983;Salimova et al, 1987;Rathouz and Kirk, 1988). No fluorescence was observed with green excitation.…”

Section: Ca Histofluorescence and Immunohistochemistrymentioning

confidence: 99%

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Colocalization of γ‐aminobutyric acid‐like immunoreactivity and catecholamines in the feeding network of Aplysia californica

Díaz‐Ríos

Oyola

Miller

2002

J of Comparative Neurology

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Functional consequences of neurotransmitter coexistence and cotransmission can be readily studied in certain experimentally favorable invertebrate motor systems. In this study, whole-mount histochemical methods were used to identify neurons in which gamma-aminobutyric acid (GABA)-like immunoreactivity (GABAli) was colocalized with catecholamine histofluorescence (CAh; FaGlu method) and tyrosine hydroxylase (TH)-like immunoreactivity (THli) in the feeding motor circuitry (buccal and cerebral ganglia) of the marine mollusc Aplysia californica. In agreement with previous reports, five neurons in the buccal ganglia were found to exhibit CAh. These included the paired B20 buccal-cerebral interneurons (BCIs), the paired B65 buccal interneurons, and an unpaired cell with projections to both cerebral-buccal connectives (CBCs). Experiments in which the FaGlu method was combined with the immunohistochemical detection of GABA revealed double labeling of all five of these neurons. An antibody generated against TH, the rate-limiting enzyme in the biosynthesis of catecholamines, was used to obtain an independent determination of GABA-CA colocalization. Biocytin backfills of the CBC performed in conjunction with TH immunohistochemistry revealed labeling of the rostral B20 cell pair and the unpaired CBI near the caudal surface of the right hemiganglion. THli was also present in a prominent bilateral pair of caudal neurons that were not stained with CBC backfills. On the basis of their position, size, shape, and lack of CBC projections, the lateral THli neurons were identified as B65. Double-labeling immunohistochemical experiments revealed GABAli in all five buccal THli neurons. Finally, GABAli was observed in individual B20 and B65 neurons that were identified using electrophysiological criteria and injected with a marker (neurobiotin). Similar methods were used to demonstrate that a previously identified catecholaminergic cerebral-buccal interneuron (CBI) designated CBI-1 contained THli but did not contain GABAli. Although numerous THli and GABAli neurons and fibers were present in the cerebral and buccal ganglia, additional instances of their colocalization were not observed. These findings indicate that GABA and a catecholamine (probably dopamine) are colocalized in a limited number of interneurons within the central pattern generator circuits that control feeding-related behaviors in Aplysia.

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Section: Colocalization Of Catecholaminergic Markersmentioning

confidence: 54%

Section: Colocalization Of Catecholaminergic Markersmentioning

confidence: 99%

Section: Ca Histofluorescence and Immunohistochemistrymentioning

confidence: 99%

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Colocalization of γ‐aminobutyric acid‐like immunoreactivity and catecholamines in the feeding network of Aplysia californica

Díaz‐Ríos

Oyola

Miller

2002

J of Comparative Neurology

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“…1D), but its position was more often skewed in one direction or the other. Unpaired buccal neurons have been described previously in the buccal system of Aplysia Rathouz and Kirk, 1988;Kabotyanski et al, 1998), but none have been physiologically characterized to date. In the pond snail Lymnaea stagnalis, the unpaired cholinergic slow oscillator (SO) interneuron has been intensively studied and is thought to act as a modulatory element of the feeding circuit (Rose and Benjamin, 1981;Elliott and Benjamin, 1985;Yeoman et al, 1993).…”

Section: Gabaergic Cells and Circuitsmentioning

confidence: 96%

“…Within the buccal ganglion, there are notable similarities between the distribution of GABAi neurons and the staining pattern observed by using histological methods to detect catecholamines. An unpaired dopaminergic medial cell (Rathouz and Kirk, 1988;Goldstein and Schwartz, 1989) with projections in both CBCs (Kabotyanski, 1998) and a bilateral pair of rostral dopaminergic interneurons with bilateral CBC projections (the B20 cell pair; Teyke et al, 1993) have been described. With the possible exception of these cells and the two immunoreactive cerebral-buccal interneurons (see below), the map of GABAi cells does not appear to include neurons that have been characterized previously.…”

Section: Gabaergic Cells and Circuitsmentioning

confidence: 99%

Localization of GABA-like immunoreactivity in the central nervous system ofAplysia californica

1999

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Localization of biogenic amines in the foregut of Aplysia californica: Catecholaminergic and serotonergic innervation

Martinez-Rubio

Serrano

2009

J of Comparative Neurology

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This study examined the catecholaminergic and serotonergic innervation of the foregut of Aplysia californica, a model system in which the control of feeding behaviors can be investigated at the cellular level. Similar numbers (15-25) of serotonin-like-immunoreactive (5HTli) and tyrosine hydroxylase-like-immunoreactive (THli) fibers were present in each (bilateral) esophageal nerve (En), the major source of pregastric neural innervation in this system. The majority of En 5HTli and THli fibers originated from the anterior branch (En2), which innervates the pharynx and the anterior esophagus. Fewer fibers were present in the posterior branch (En1), which innervates the majority of the esophagus and the crop. Backfills of the two En branches toward the central nervous system (CNS) labeled a single, centrifugally projecting serotonergic fiber, originating from the metacerebral cell (MCC). The MCC fiber projected only to En2. No central THli neurons were found to project to the En. Surveys of the pharynx and esophagus revealed major differences between their patterns of catecholaminergic (CA) and serotonergic innervation. Whereas THli fibers and cell bodies were distributed throughout the foregut, 5HTli fibers were present in restricted plexi, and no 5HTli somata were detected. Double-labeling experiments in the periphery revealed THli neurons projecting toward the buccal ganglion via En2. Other afferents received dense perisomatic serotonergic innervation. Finally, qualitative and quantitative differences were observed between the buccal motor programs (BMPs) produced by stimulation of the two En branches. These observations increase our understanding of aminergic contributions to the pregastric regulation of Aplysia feeding behaviors.

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Localization of catecholamines in the buccal ganglia ofAplysia californica

Cited by 22 publications

References 37 publications

Colocalization of γ‐aminobutyric acid‐like immunoreactivity and catecholamines in the feeding network of Aplysia californica

Colocalization of γ‐aminobutyric acid‐like immunoreactivity and catecholamines in the feeding network of Aplysia californica

Localization of GABA-like immunoreactivity in the central nervous system ofAplysia californica

Localization of biogenic amines in the foregut of Aplysia californica: Catecholaminergic and serotonergic innervation

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