Localization of a Crustacean Hyperglycemic Hormone-like Immunoreactivity in the Neuroendocrine System ofEuscorpius carpathicus(L.) (Scorpionida, Chactidae)

Stöckmann, Ricarda; Laverdure, Anne-Marie; Breuzet, M.

doi:10.1006/gcen.1997.6874

Cited by 3 publications

(1 citation statement)

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“…As for decapod CHHs, ITP genes produce an ITP-L isoform by alternative splicing, whose function remains elusive. To go further, heterologous immunostainings revealed the occurrence of CHH-like peptides in two branchiopods [15], a myriapod [16] and a chelicerate [17], and a gene was found in the genome of the nematode Caenorhabditis elegans , which encodes a putative peptide exhibiting a CHH family structural signature [3]. To increase our knowledge on the CHH family in the taxa cited above, we have conducted an in silico study on available genomic sequences from arthropods and nematodes.…”

Section: Introductionmentioning

confidence: 99%

Molecular evolution of the crustacean hyperglycemic hormone family in ecdysozoans

et al. 2010

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BackgroundCrustacean Hyperglycemic Hormone (CHH) family peptides are neurohormones known to regulate several important functions in decapod crustaceans such as ionic and energetic metabolism, molting and reproduction. The structural conservation of these peptides, together with the variety of functions they display, led us to investigate their evolutionary history. CHH family peptides exist in insects (Ion Transport Peptides) and may be present in all ecdysozoans as well. In order to extend the evolutionary study to the entire family, CHH family peptides were thus searched in taxa outside decapods, where they have been, to date, poorly investigated.ResultsCHH family peptides were characterized by molecular cloning in a branchiopod crustacean, Daphnia magna, and in a collembolan, Folsomia candida. Genes encoding such peptides were also rebuilt in silico from genomic sequences of another branchiopod, a chelicerate and two nematodes. These sequences were included in updated datasets to build phylogenies of the CHH family in pancrustaceans. These phylogenies suggest that peptides found in Branchiopoda and Collembola are more closely related to insect ITPs than to crustacean CHHs. Datasets were also used to support a phylogenetic hypothesis about pancrustacean relationships, which, in addition to gene structures, allowed us to propose two evolutionary scenarios of this multigenic family in ecdysozoans.ConclusionsEvolutionary scenarios suggest that CHH family genes of ecdysozoans originate from an ancestral two-exon gene, and genes of arthropods from a three-exon one. In malacostracans, the evolution of the CHH family has involved several duplication, insertion or deletion events, leading to neuropeptides with a wide variety of functions, as observed in decapods. This family could thus constitute a promising model to investigate the links between gene duplications and functional divergence.

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