Localization of 5-HT7 receptors in rat brain by immunocytochemistry, in situ hybridization, and agonist stimulated cFos expression

Neumaier, John F.; Sexton, Timothy J.; Yracheta, Joseph; Diaz, Arturo; Brownfield, Mark S.

doi:10.1016/s0891-0618(00)00092-2

Cited by 187 publications

(136 citation statements)

References 30 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Experiment 1. The effect of aging on 5-HT 7 receptor mRNA expression in the dorsal raphe and other discrete regions of the midbrain and the forebrain Expression of 5-HT 7 receptor mRNA was observed in many brain regions, similar to previous findings in rats (Neumaier et al, 2001). The DRN exhibited a relatively low level of 5-HT 7 receptor mRNA expression that was not significantly affected by aging ( Fig.…”

Section: Resultssupporting

confidence: 88%

Expression of 5-HT7 receptor mRNA in the hamster brain: Effect of aging and association with calbindin-D28K expression

Duncan

Franklin

2007

Brain Research

View full text Add to dashboard Cite

Aging affects several processes modulated by the 5-HT 7 receptor subtype, including circadian rhythms, learning and memory, and depression. Previously, we showed that aging induces a decrease in the hamster dorsal raphe (DRN) in both 5-HT 7 receptor binding and circadian phase resetting responses to 8-OH-DPAT microinjection. To elucidate the mechanisms underlying the aging decrease in 5-HT 7 receptors, we investigated aging modulation of 5-HT 7 receptor mRNA expression in the DRN, brain regions afferent to the DRN, and brain regions regulating circadian rhythms or memory. In situ hybridization for 5-HT 7 receptor mRNA was performed on coronal sections prepared from the brains of young, middle-aged, and old male Syrian hamsters. 5-HT 7 receptor mRNA expression was quantified by densitometry of X-ray film autoradiograms. The results showed that aging did not significantly affect 5-HT 7 receptor mRNA expression in the DRN or most other brain regions examined, with the exception of the cingulate cortex and paraventricular thalamic nucleus. Within the suprachiasmatic nucleus, the site of the master circadian pacemaker in mammals, 5-HT 7 receptor mRNA expression was localized in a discrete subregion resembling the calbindin subnucleus previously described. A second experiment using adjacent tissue sections showed that 5-HT 7 receptor mRNA and calbindin mRNAs were concentrated in the same region of the SCN, and as well as in the same region of several other brain structures. The localization of 5-HT 7 receptors and calbindin mRNAs within the same regions suggests that proteins they encode may interact to modulate processes such as circadian timekeeping.

show abstract

Section: Resultssupporting

confidence: 88%

Expression of 5-HT7 receptor mRNA in the hamster brain: Effect of aging and association with calbindin-D28K expression

Duncan

Franklin

2007

Brain Research

View full text Add to dashboard Cite

show abstract

“…In situ hybridization and immunohistochemical studies show that the mRNA and receptor protein have a similar distribution in discrete regions of the CNS including the cortex, hippocampus, and amygdala (Neumaier et al, 2001). As previously mentioned, several antipsychotic drugs have high affinity for these 5-HT7 receptors (Roth et al, 1994).…”

Section: -Ht7mentioning

confidence: 83%

Pharmacologic mechanisms of serotonergic regulation of dopamine neurotransmission

Alex

Pehek

2007

Pharmacology & Therapeutics

526

311

View full text Add to dashboard Cite

The neurotransmitter dopamine (DA) has a long association with normal functions such as motor control, cognition, and reward, as well as a number of syndromes including drug abuse, schizophrenia, and Parkinson's disease. Studies show that serotonin (5-HT) acts through several 5-HT receptors in the brain to modulate DA neurons in all three major dopaminergic pathways. There are at least 14 5-HT receptor subtypes, many of which have been shown to play some role in mediating 5-HT/DA interactions. Several subtypes, including the 5-HT1A, 5-HT1B, 5-HT2A, 5-HT3 and 5-HT4 receptors, act to facilitate DA release, while the 5-HT2C receptor mediates an inhibitory effect of 5-HT on DA release. Most 5-HT receptor subtypes only modulate DA release when 5-HT and/or DA neurons are stimulated, but the 5-HT2C receptor, characterized by high levels of constitutive activity, inhibits tonic as well as evoked DA release. This review summarizes the anatomical evidence for the presence of each 5-HT receptor subtype in dopaminergic regions of the brain and the neuropharmacological evidence demonstrating regulation of each DA pathway. The relevance of 5-HT receptor modulation of DA systems to the development of therapeutics used to treat schizophrenia, depression, and drug abuse is discussed. Lastly, areas are highlighted in which future research would be maximally beneficial to the treatment of these disorders.

show abstract

“…However, in 5-HT 4 -deficient mice, chronic fluoxetine significantly enhanced the dopaminergic modulation as in wild-type mice. The serotonin 5-HT 6 and 5-HT 7 receptors, which are also coupled to Gs-cAMP cascades, have been reported to be expressed in the dentate gyrus (Gérard et al, 1997;Neumaier et al, 2001;Vizuete et al, 1997). These receptors might have compensated for the lack of the 5-HT 4 receptor in the mutant mice.…”

Section: Discussionmentioning

confidence: 99%

Chronic Fluoxetine Selectively Upregulates Dopamine D1-Like Receptors in the Hippocampus

Kobayashi

Haneda

Higuchi³

et al. 2012

Neuropsychopharmacol

View full text Add to dashboard Cite

The dentate gyrus of the hippocampus has been implicated in mechanisms of action of selective serotonin reuptake inhibitors (SSRIs). We have recently demonstrated that the SSRI fluoxetine can reverse the state of maturation of the adult dentate granule cells and enhances serotonin 5-HT 4 receptor-mediated synaptic potentiation at the synapses formed by their mossy fiber axons. Here, we show that fluoxetine can induce long-lasting enhancement of dopaminergic modulation at the mossy fiber synapse. Synaptic responses arising from the mossy fiber-CA3 pyramidal cell synapse were recorded using acute mouse hippocampal slices. Dopamine potentiates mossy fiber synaptic transmission by activating D 1 -like receptors. Chronic fluoxetine treatment induced a prominent increase in the magnitude of dopamine-induced synaptic potentiation, and this effect was maintained at least up to 1 month after withdrawal of fluoxetine. Quantitative autoradiography revealed that binding of the D 1 -like receptor ligand [ 3 H]SCH23390 was selectively increased in the dentate gyrus and along the mossy fiber in fluoxetine-treated mice. However, binding of the 5-HT 4 receptor ligand [ 3 H]GR113808 was not significantly changed. These results suggest that chronic fluoxetine enhanced the dopaminergic modulation at least in part by upregulating expression of D 1 -like receptors, while the enhanced serotonergic modulation may be mediated by modifications of downstream signaling pathways. These enhanced monoaminergic modulations would greatly increase excitatory drive to the hippocampal circuit through the dentate gyrus. The highly localized upregulation of D 1 -like receptors further supports the importance of the dentate gyrus in the mechanism of action of SSRIs.

show abstract

Localization of 5-HT7 receptors in rat brain by immunocytochemistry, in situ hybridization, and agonist stimulated cFos expression

Cited by 187 publications

References 30 publications

Expression of 5-HT7 receptor mRNA in the hamster brain: Effect of aging and association with calbindin-D28K expression

Expression of 5-HT7 receptor mRNA in the hamster brain: Effect of aging and association with calbindin-D28K expression

Pharmacologic mechanisms of serotonergic regulation of dopamine neurotransmission

Chronic Fluoxetine Selectively Upregulates Dopamine D1-Like Receptors in the Hippocampus

Contact Info

Product

Resources

About