Localisation of Arabidopsis NDPK2—revisited

Abstract

Phytochromes are light responsive photoreceptors in plants that influence development and differentiation during the entire plant life cycle. Plant nucleoside diphosphate kinase 2 (NDPK2) has been reported to be a component of the light-mediated signalling cascade and to interact physically with phytochrome A in the cytosol. By using diverse methods as in vitro imports, in vivo localisation of GFP-fusion proteins and immuno blotting of plant cell fractions we clearly localise NDPK2 only to chloroplasts but not to the cytosol, demonstrating that although high affinity protein-protein interactions can occur in vitro, their physiological relevance can be artificial if the proteins are localised to different cell compartments in vivo.

ROS resistance in Pisum sativum cv. Alaska: the involvement of nucleoside diphosphate kinase in oxidative stress responses via the regulation of antioxidants

“…Since CAT has been reported to be predominantly present in the peroxisome (Corpas et al 1999), it has been postulated that the CAT inactivation reaction occurs outside of the chloroplast and is mediated by the released reactive oxygen produced in the chloroplasts under high excitation pressure (Shang and Feierabend 1999), whereas NDPK2 was reported to localize to the chloroplast stroma in pea (Lübeck and Soll 1995). Therefore, the possibility of interaction between pea CAT and NDPK2 can be detected in vitro; however, it has been proposed that interactions detected in vitro may not necessarily occur in vivo, if the proteins are located in different compartments (Bölter et al 2007). We propose as alternative interpretation that just before entering their destined organelle, NDPK2 and CAT1 may interact (Fig.…”

New insights into the subcellular localization of Tubby-like proteins and their participation in theArabidopsis-Piriformospora indicainteraction

“…This would not only enhance our knowledge on this interesting protein family but will also to GFP while a nucleo-cytosolic localization was reported when GFP was placed N-terminal to AtNDPK2. 31,32 Therefore, we examined possible positional effects of GFP on the localization of AtTLP3. For this, GFP was placed in front of the N-terminal part of AtTLP3 (GFP-AtTLP3Δ116-406), or C-terminal to its Tubby domain (AtTLP3Δ1-115-GFP), reversing its position in comparison to the AtTLP3 versions described above.…”

Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

“…37) However, a later report confirmed that AtNDPK2 is localized to the chloroplasts. 15) Furthermore, a type II NDPK from pea was purified from the leaf chloroplast. 16) Localization of AtNDPK3 is also unclear, because three groups have reported differently that AtNDPK3 is localized to the mitochondria, the chloroplasts, and both.…”

“…8) Three types of NDPKs have been identified in plants based on their amino acid sequences. [9][10][11][12][13][14] Type I NDPK is a cytosolic protein, 11,14) while type II and type III NDPK bear an N-terminal extension likely to be involved in targeting of the proteins to chloroplasts [15][16][17][18] or mitochondria. 19,20) Plant NDPKs have also been found to be multi-functional proteins, and to be strongly related to signal transduction, differentiation, and development.…”