Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (<i>Oryza sativa</i>L.)

Kihara, Akihiko; Saburi, Wataru; Wakuta, Shinji; Kim, Myung‐Hee; Hamada, Shigeki; Ito, Hiroyuki; Imai, Ryozo; Matsui, Hirokazu

doi:10.1271/bbb.110285

Cited by 16 publications

(14 citation statements)

References 32 publications

(42 reference statements)

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“…Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme functioning in intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthesis pathways and regulatory functions in cells [43,44]. Plant NDPKs are also involved in signal transduction, differentiation and development [45-47].…”

Section: Discussionmentioning

confidence: 99%

Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

et al. 2012

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BackgroundThe analyses of protein synthesis, accumulation and regulation during grain development in wheat are more complex because of its larger genome size compared to model plants such as Arabidopsis and rice. In this study, grains from two wheat cultivars Jimai 20 and Zhoumai 16 with different gluten quality properties were harvested at five development stages, and were used to displayed variable expression patterns of grain proteins.ResultsProteome characterization during grain development in Chinese bread wheat cultivars Jimai 20 and Zhoumai 16 with different quality properties was investigated by 2-DE and tandem MALDI-TOF/TOF-MS. Identification of 117 differentially accumulated protein spots representing 82 unique proteins and five main expression patterns enabled a chronological description of wheat grain formation. Significant proteome expression differences between the two cultivars were found; these included 14 protein spots that accumulated in both cultivars but with different patterns and 27 cultivar-different spots. Among the cultivar-different protein spots, 14 accumulated in higher abundance in Jimai 20 than in Zhoumai 16, and included NAD-dependent isocitrate dehydrogenase, triticin precursor, LMW-s glutenin subunit and replication factor C-like protein. These proteins are likely to be associated with superior gluten quality. In addition, some proteins such as class II chitinase and peroxidase 1 with isoforms in developing grains were shown to be phosphorylated by Pro-Q Diamond staining and phosphorprotein site prediction. Phosphorylation could have important roles in wheat grain development. qRT-PCR analysis demonstrated that transcriptional and translational expression patterns of many genes were significantly different.ConclusionsWheat grain proteins displayed variable expression patterns at different developmental stages and a considerable number of protein spots showed differential accumulation between two cultivars. Differences in seed storage proteins were considered to be related to different quality performance of the flour from these wheat cultivars. Some proteins with isoforms were phosphorylated, and this may reflect their importance in grain development. Our results provide new insights into proteome characterization during grain development in different wheat genotypes.

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Section: Discussionmentioning

confidence: 99%

Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

et al. 2012

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“…Whereas the ATP and GTP pathways diverge at IMP in all living organisms, the enzymes at the branch point are thought to localize to different compartments in plant cells, with adenylosuccinate synthase localizing to plastids and likely also to mitochondria and IMP dehydrogenase being restricted to the cytosol (17)(18)(19). In contrast to ATP biosynthesis in organelles, two distinct sets of enzymes (GK and nucleoside diphosphate kinase) that are required for the conversion of GMP to GTP are present in both chloroplasts and the cytosol (20,21). As a result of this disjunction at GMP, GKpm appears to be responsible for the first step of GTP biosynthesis in plant chloroplasts (Fig.…”

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confidence: 99%

Diversity in Guanosine 3′,5′-Bisdiphosphate (ppGpp) Sensitivity among Guanylate Kinases of Bacteria and Plants

Nomura

Izumi

Fukunaga

et al. 2014

Journal of Biological Chemistry

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Background:The ppGpp signaling system is operative in plant chloroplasts and bacteria. Results: Chloroplast and cytosolic guanylate kinases (GKs) of plants are sensitive and insensitive to ppGpp, respectively, whereas bacterial GKs show diversity in ppGpp sensitivity. Conclusion: GTP biosynthesis in chloroplasts is controlled by ppGpp. Significance: Identification of the targets of ppGpp should provide insight into biological processes regulated by this nucleotide.

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“…NDPK (spot 21) is a protein responsible for cellular organization, regulating the nucleotide groups in the cells (Kihara et al, 2011). It has been reported to be involved in signal transduction in plants.…”

Section: Resultsmentioning

confidence: 99%

Analysis of protein profile of tomato root infected with Fusarium oxysporum f. sp lycopersici

Silva¹,

Almeida²,

Malafaia³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by two-dimensional electrophoresis followed by staining with Coomassie brilliant blue. The proteins were identified by MALDI-TOF/TOF mass spectrometry. A total of 22 proteins were identified, 21 of which showed differential expression with 12 proteins being upregulated and nine being downregulated. Plants responded to the pathogen with increased expression of pathogenesis-related proteins. We noted the induction of proteins involved in hypersensitivity reaction and other defense mechanisms. The expression of proteins of primary metabolism related to energy production, however, decreased, as did the expression of two proteins related to defense against abiotic stress. These results demonstrate the presence of important mechanisms for defense against Fol in the tomato genotype BHRS 2,3.

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Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

Cited by 16 publications

References 32 publications

Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.)

Diversity in Guanosine 3′,5′-Bisdiphosphate (ppGpp) Sensitivity among Guanylate Kinases of Bacteria and Plants

Analysis of protein profile of tomato root infected with Fusarium oxysporum f. sp lycopersici

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