Local supply of reactive oxygen species into a tissue model by atmospheric-pressure plasma-jet exposure

Kawasaki, Toshiyuki; Mitsugi, Fumiaki; Koga, Kazunori; Shiratani, Masaharu

doi:10.1063/1.5091740

Cited by 17 publications

(14 citation statements)

References 49 publications

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“…In terms of RONS transport, Szili et al showed that the delivery of RONS in a liquid through a gel barrier is markedly affected by the barrier thickness [43,72], both in terms of time lag and total RONS concentration in the underlying liquid. Similarly, Kawasaki et al showed the same phenomenon with a gel barrier + reagent model where both RONS concentrations and distribution were drastically changed when varying the thickness from 0.5 mm to 3 mm [65]. Additionally, it will have an influence on the absorbance measurement used to analyze reporters, as discussed in section 3.2.2.…”

Section: Liquid Mediummentioning

confidence: 76%

“…• Cell-related mechanisms: in addition to intracellular generation, reporters do not render the other cellular mechanisms including signaling pathways (cell-to-cell communication, cascade effect [143]). • The distribution pattern of RONS significantly varies (pointlike, circular, ring-shaped or even star-shaped [57]) depending on multiple factors (gas mix [11,47,58], distance [43,47,64,65]) or flow [42,47]. This distribution also varies along the depth of the tissue [47,64,65].…”

Section: Limitationsmentioning

confidence: 99%

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Gel models to assess distribution and diffusion of reactive species from cold atmospheric plasma: an overview for plasma medicine applications

Thulliez¹,

Bastin²,

Nonclercq³

et al. 2021

J. Phys. D: Appl. Phys.

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The emerging field of plasma medicine opens new therapeutic opportunities with the use of Cold Atmospheric Plasma (CAP) as a versatile tool for the treatment of tissues in various medical indications. Yet, the complexity of this very reactive medium combined with a high dependence on its environment and generation parameters make it difficult to predict and optimize such treatment. To this end, a simple yet robust and accurate tissue phantom allowing to study the penetration and distribution of plasma action in simulated in vivo conditions has been developed by several groups. It combines a hydrogel-based matrix closely resembling tissues and chemical reporters incorporated in the gel to measure the delivery of Reactive Oxygen and Nitrogen Species (RONS) by the plasma. This paper reports the use of these models in the literature to give an overview of the state of the art, their capabilities and the further research required to improve it. First the hydrogels composition (i.e. gelatin and agarose) is discussed, as well as the parameters allowing to fine tune the model. In particular, we show that modifying mass fraction has been reported to mimic several types of tissues and that different model configurations allow to test different treatments conditions, including the barrier effect of skin or the direct treatment of a tissue bulk. The role of other critical parameters is highlighted, including manufacturing, diffusion, electrical characteristics but also liquid composition, thickness, aging and temperature influence. Secondly, RONS reporters used in the plasma medicine literature (colorimetric/fluorometric dyes) are summarized. The analysis techniques are discussed and the dyes characteristics (i.e. wavelength, specificity, concentrations) are reported. Finally, the influence of medium and time on these measurements are covered. For both sections, the limitations of these current models are presented and linked to potential improvements and further research.

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Section: Liquid Mediummentioning

confidence: 76%

Section: Limitationsmentioning

confidence: 99%

Gel models to assess distribution and diffusion of reactive species from cold atmospheric plasma: an overview for plasma medicine applications

Thulliez¹,

Bastin²,

Nonclercq³

et al. 2021

J. Phys. D: Appl. Phys.

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“…The gel reagent allows a two-dimensional (2D) ROS concentration distribution to be easily visualized even in a liquid. The 2D distributions of the ROS after passing through water [30][31][32] and a tissue model [33][34][35] have already been reported under various experimental conditions. However, it is difficult to identify the ROS detected by the proposed method because all of them induce the same color reactions.…”

Section: Introductionmentioning

confidence: 93%

Experimental identification of the reactive oxygen species transported into a liquid by plasma irradiation

Kawasaki

Koga

Shiratani

2020

Jpn. J. Appl. Phys.

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Reactive oxygen species (ROS) supplied into liquids by plasma irradiation must be identified and controlled for the safe and effective use of plasmas in biomedical applications. This study aims to obtain information on the key ROS regarding ROS transportation through a liquid layer for realization of spatial ROS identification. Two-dimensional distributions of ROS after passing through liquids with and without ROS scavengers were visualized and compared to assess the suppression effects of each scavenger using a measurement system combined with a gel ROS detector. The plasma was generated under helium and 1% oxygen mixture gases. It was experimentally confirmed that a hydroxyl radical is not a key species, whereas, superoxide anion radicals and hydrogen peroxide play a key role for ROS transportation in our system. The results also suggested that the chemical processes for ROS transportation change at a liquid layer thickness of less than 1 mm.

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“…Figure 6b illustrates the operation of this four multi-jet device exposing the agar sample [49] enriched with potassium iodide. The potassium iodide is used as an indicator for the delivery of reactive species by the multi-jets [44,50]. Is it shown that for a 60 s plasma exposure, the reactive species are delivered as four spots on the sample while for a longer exposure of 240 s, the reactive species are detected almost all over a disk defined by the edges of the four multi-jet assembly.…”

Section: Multi-jets Characterizationmentioning

confidence: 99%

Anti-Bacterial Action of Plasma Multi-Jets in the Context of Chronic Wound Healing

et al. 2021

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This work is a contribution to the development and implementation of non-thermal plasma technology for decontamination in the perspective of nosocomial and chronic wound innovative therapies. Multi jets devices based on Plasma Gun® technology in static and scanning operation modes and bacterial lawns inoculated with resistant and non-resistant bacterial strains were designed and used. A pilot toxicity study exploring plasma treatment of wound bearing patients, performed with a low voltage plasma applicator, is documented as a first step for the translation of in vitro experiments to clinical care. Bacterial inactivation was demonstrated for Staphylococcus aureus, Pseudomonas aeruginosa and drug resistant S. aureus, P. aeruginosa and Escherichia Coli strains collected from patient wounds at Orleans (France) hospital. A few square centimeter large contaminated samples were inactivated following a single plasma exposure as short as one minute. Samples inoculated with a single but also a mix of three resistant pathogens were successfully inactivated not only right after their contamination but for mature lawns as well. Similar bactericidal action was demonstrated for antibiotic-resistant and non-resistant P. aeruginosa. The time exposure dependent increase of the inhibition spots, following multi jets exposure, is discussed as either the accumulation of reactive species or the likely combinatory action of both the reactive species and transient electric field delivery on inoculated samples.

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Local supply of reactive oxygen species into a tissue model by atmospheric-pressure plasma-jet exposure

Cited by 17 publications

References 49 publications

Gel models to assess distribution and diffusion of reactive species from cold atmospheric plasma: an overview for plasma medicine applications

Gel models to assess distribution and diffusion of reactive species from cold atmospheric plasma: an overview for plasma medicine applications

Experimental identification of the reactive oxygen species transported into a liquid by plasma irradiation

Anti-Bacterial Action of Plasma Multi-Jets in the Context of Chronic Wound Healing

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