Local Production of Antibody by the Lactating Mammary Gland of the Ewe and the Effect of Systemic Immunization

Gh, McDowell; Lascelles, A. K.

doi:10.1016/s0034-5288(18)34202-4

Cited by 9 publications

(2 citation statements)

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“…Recently we proposed that systemic injection of antigen during early involution could result in specifically-sensitized lymphoid cells entering involuting mammary tissue in the lymphocytic infiltrate (McDowell & Lascelles 1971a). This arose from the following observations.…”

Section: Cellular Basis For Local Immunitymentioning

confidence: 99%

Localized Humoral Immunity with Particular Reference to Ruminants

Lascelles

McDowell

1974

Immunological Reviews

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Section: Cellular Basis For Local Immunitymentioning

confidence: 99%

Localized Humoral Immunity with Particular Reference to Ruminants

Lascelles

McDowell

1974

Immunological Reviews

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“…Circulating antigen may have entered the mammary gland and elicited a local immune response, although this seems most unlikely. Alternatively, antigen-stimulated lymphoblasts may have migrated into the mammary gland, a possibility favored by Lascelles and co-workers from studies in sheep (18,21) and cattle (41).…”

Section: Average Weekly Increases In Antibody Levels In Preparturientmentioning

confidence: 99%

Immune Response in the Bovine Mammary Gland After Intestinal, Local, and Systemic Immunization

1981

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The immune response in mammary glands of cattle was measured after intestinal, local, and systemic immunization with T4 bacteriophage. Nonlactating pregnant cows were immunized by infusions into the intestine or mammary gland and by subcutaneous injections in the region of the prescapular or external inguinal lymph nodes. Titers of antibodies of different isotypes were measured in serum and in lacteal secretions by enzyme-linked immunosorbent assay, and numbers of cells producing antibodies of each isotype were determined in lacteal secretions by the Jerne plaque assay. Substantial increases in immunoglobulin G subclass 1 (IgGl) and IgG2 antibody titers were detected in serum and lacteal secretions of animals immunized through an intestinal fistula. IgM and IgA antibody responses were low or undetectable. Low numbers of IgA and IgGl plaque-forming cells were occasionally detected. It is proposed on the basis of these data that migration of antigen-stimulated IgG lymphoblasts, and perhaps of antigen, to spleen and peripheral lymph nodes may be dominant events after intestinal immunization of ruminants. This is consistent with the predominance of serum-derived IgG antibodies in colostrum and milk. Intramammary infusion of antigen gave rise to increases in antibody titers in all classes which were greater not only in lacteal secretions but also in blood serum than with either systemic route used. There was clear evidence from relative antibody titers for local synthesis of antibodies, principally IgA and IgGl, in the immunized glands. Comparison of IgA titers in secretions from the immunized glands with those in serum also suggested that locally synthesized IgA antibodies may have contributed in some measure to serum titers. Local synthesis in both immunized and nonimmunized glands was also reflected by the presence of increased numbers of IgA and IgGl plaque-forming cells. It is hypothesized that antibody-forming cells responsible for local synthesis originated in lymphoid tissue within the mammary gland or from peripheral lymph nodes, depending upon the route of immunization.During the past decade important advances have been made in understanding migration patterns of B lymphoblasts within the secretory immune system. Antibody-forming precursor cells from Peyer's patches give rise in rabbits to plasma cells synthesizing immunoglobulin A (IgA) in the lamina propria of the intestine (7,8,34,35) as well as in the bronchi (34). A reciprocal migration was observed by cells from bronchusassociated lymphoid tissue (34). In several species, including humans, rats, rabbits, and swine, oral immunization during pregnancy has been shown to result in the presence of antibodies, predominantly IgA, in colostrum and milk (3,13,24,25,36). Oral immunization of women with Escherichia coli resulted in a marked increase of cells forming 0 antibodies of the IgA class in preparturient lacteal secretions and in colostrum t Present address:

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