Local light signaling at the leaf tip drives remote differential petiole growth through auxin-gibberellin dynamics

Küpers, Jesse J; Snoek, Basten L.; Oskam, Lisa; Pantazopoulou, Chrysoula K; Matton, Sanne E.A.; Reinen, Emilie; Liao, Che‐Yang; Eggermont, Eline; Weekamp, Harold; Biddanda-Devaiah, Muthanna; Kohlen, Wouter; Weijers, Dolf; Pierik, R.L.M.

doi:10.1016/j.cub.2022.11.045

Cited by 26 publications

(46 citation statements)

References 56 publications

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“…Thus, as a downstream target of the PIF4/PIF5 activity, ATHB2 appears to coordinate the synthesis of GA which is an essential component of the early-stage shade avoidance response. While it is not clear whether the induction of ATHB2 is a result of direct activation by PIF4/PIF5 during the procedure or the one requiring the auxin biosynthesis as a prerequisite as was recently proposed (Küpers et al, 2023), ATHB2 seems to play a key role in bridging the auxin and GA signaling together to facilitate the shade avoidance response.…”

Section: Discussionmentioning

confidence: 93%

Identification of GA20ox2 as a target of ATHB2 and TCP13 during shade response

et al. 2023

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The shade avoidance syndrome (SAS) is a collective adaptive response of plants under shade highlighted by characteristic phenotypes such as hypocotyl elongation, which is largely mediated by concerted actions of auxin and GA. We identified ATHB2, a homeodomain-leucine zipper (HD-Zip) domain transcription factor known to be rapidly induced under shade condition, as a positive regulator of GA biosynthesis necessary for the SAS by transactivating the expression of GA20ox2, a key gene in the GA biosynthesis pathway. Based on promoter deletion analysis, EMSA and ChIP assay, ATHB2 appears to regulate the GA20ox2 expression as a direct binding target. We also found that the GA20ox2 expression is under negative control by TCP13, the effect of which can be suppressed by presence of ATHB2. Considering a rapid induction kinetics of ATHB2, this relationship between ATHB2 and TCP13 may allow ATHB2 to play a shade-specific activator for GA20ox by derepressing a pre-existing activity of TCP13.

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Section: Discussionmentioning

confidence: 93%

Identification of GA20ox2 as a target of ATHB2 and TCP13 during shade response

et al. 2023

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“…Since LBD16 was previously shown to be involved in auxin-regulated LR development 14 , we first addressed the question whether salt would affect auxin signaling during LR development. We used a high-resolution C3PO auxin reporter line that contains a threecolor reporter by incorporating a DR5v2::n3mTurqoise2 (auxin response) cassette into the construct carrying the R2D2 (auxin input) cassette (pDR5v2:mTurquoise-NLS/pRPS5a:mDII:ntdTomato/pRPS5a:DII:n3xVenus) to visualize auxin dynamics at cellular level during LR development under salt stress 23 . We found that DR5v2 activity was reduced in the root zones including meristem, elongation and early differentiation zones after 6 h treatment with 125 mM NaCl (Fig.…”

Section: Salinity Activates An Auxin-independent Pathway Mediating Lb...mentioning

confidence: 99%

“…All the Arabidopsis thaliana wild-type, mutant and transgenic lines used in this research are in Columbia-0 (Col-0) ecotype background, except for the auxin reporter line C3PO (in Col-Utrecht ecotype) 23 . T-DNA knock-out insertion lines of lbd16-1 (SALK_095791), lbd16-2 (SALK_040739) were obtained from the lab of Prof. Malcolm Bennett (University of Nottingham) and Prof. Ben Scheres (Wageningen University& Research), respectively.…”

Section: Plant Materials Growth Conditions and Stress Treatmentsmentioning

confidence: 99%

“…For auxin response analysis during salt stress, seven-day-old Arabidopsis Col-0 seedlings carrying a triple color auxin reporter C3PO line 23 were transferred to 0.5x MS liquid media containing 0 mM or 125 mM NaCl and placed on a shaker for 6 h. After treatment, roots were fixed in PFA and cleared overnight using ClearSee solution. Cleared roots were mounted on slides with ClearSee solution.…”

Section: Confocal Microscopy and Auxin Reporter Analysismentioning

confidence: 99%

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Root branching in salt requires auxin-independent modulation of LBD16 function

Zhang

Zeeuw

et al. 2023

Preprint

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Salinity stress constrains lateral root (LR) growth and severely impacts plant growth. Auxin signaling is indispensable for the regulation of LR formation. Nevertheless, the molecular mechanism of how salinity affects root auxin signaling and whether salt would steer alternative pathway(s) to regulate LR development is unknown. Here we show that the auxin-regulated transcription factor LATERAL ORGAN BOUNDARY DOMAIN (LBD) 16, known as an essential player for LR development under control conditions, is regulated by an alternative non-canonical pathway under salinity. Salt represses auxin signaling but in parallel activates an upstream transcriptional activator of LBD16, ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6). ZAT6 modulates the activity of LBD16 to contribute to downstream cell wall remodeling, and promotes LR development under salinity stress. Our study thus shows that root developmental plasticity in response to salt stress is achieved by integration of auxin-dependent repressive and salt-activated auxin-independent pathways converging on LBD16 to modulate root branching modulation under salinity.

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“…Recent studies have shown that low R/FR ratios perceived at the leaf tip promote the accumulation of leaf tip-derived auxin in the abaxial petiole. This local auxin accumulation is responsible for the asymmetric growth that causes the hyponastic response to low R/FR, and is determined by the auxin transport protein PIN3 (Küpers et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA-mediated epigenetic regulation of auxin-related genes controlling shade avoidance syndrome inArabidopsis thaliana

Mammarella

Lucero

Hussain

et al. 2023

Preprint

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The long noncoding RNA (lncRNA) AUXIN-REGULATED PROMOTER LOOP (APOLO) recognizes a subset of target loci across the Arabidopsis thaliana genome by forming RNA-DNA hybrids (R-loop) and modulating local three-dimensional chromatin conformation. Here we show that APOLO is involved in regulating the shade avoidance syndrome (SAS) by dynamically modulating the expression of key factors. In response to far-red (FR) light, the expression of APOLO anticorrelates with its target BRANCHED1 (BRC1), a master regulator of shoot branching in Arabidopsis thaliana. APOLO deregulation results in BRC1 transcriptional repression and an increase in the number of branches. APOLO transcriptional accumulation fine-tunes the formation of a repressive chromatin loop encompassing the BRC1 promoter, which normally occurs only in leaves as well as in a late response to FR treatment in axillary buds. In addition, our data reveal that APOLO participates in leaf hyponasty, in agreement with its previously reported role in the control of auxin homeostasis through direct modulation of YUCCA2 (auxin synthesis), PID and WAG2 (auxin efflux).

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Local light signaling at the leaf tip drives remote differential petiole growth through auxin-gibberellin dynamics

Cited by 26 publications

References 56 publications

Identification of GA20ox2 as a target of ATHB2 and TCP13 during shade response

Identification of GA20ox2 as a target of ATHB2 and TCP13 during shade response

Root branching in salt requires auxin-independent modulation of LBD16 function

Long noncoding RNA-mediated epigenetic regulation of auxin-related genes controlling shade avoidance syndrome inArabidopsis thaliana

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