Root branching in salt requires auxin-independent modulation of LBD16 function

Zhang, Yanxia; Li, Yiyun; Zeeuw, Thijs de; Duijts, Kilian; Kawa, Dorota; Lamers, Jasper; Munzert, Kristina S.; Li, Hongfei; Zou, Yutao; Meyer, Andreas J.; Yan, Jinxuan; Verstappen, Francel; Wang, Yixuan; Gijsberts, Tom; Wang, Jielin; Gigli-Bisceglia, Nora; Engelsdorf, Timo; Dijk, Aalt D. J. van; Testerink, Christa

doi:10.1101/2023.04.25.538210

Cited by 3 publications

(3 citation statements)

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“…Serotonin‐treated sunflower seedlings grown on NaCl demonstrated longer primary roots than untreated seedlings (Erland et al, 2016). Recently, salinity stress has been shown to inhibit LR growth and have a negative impact on Arabidopsis development (Xu et al, 2020; Zhang et al, 2023). It has been previously demonstrated that both serotonin and melatonin induce the expression of LATERAL ORGAN BOUNDARIES DOMAIN 16 ( LBD16 ) and XYLOGLUCAN ENDOTRANSGLYCOSYLASE RELATED 6 ( XTR6 ), which promote LR growth in Arabidopsis (Wan, Zhang, Wang, et al, 2018).…”

Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

“…It has been previously demonstrated that both serotonin and melatonin induce the expression of LATERAL ORGAN BOUNDARIES DOMAIN 16 ( LBD16 ) and XYLOGLUCAN ENDOTRANSGLYCOSYLASE RELATED 6 ( XTR6 ), which promote LR growth in Arabidopsis (Wan, Zhang, Wang, et al, 2018). Both LBD16 and XYLOGLUCAN ENDOTRANSGLUCOSYLASE 23 ( XTH23 )/ XTR6 play essential roles during salinity stress (Xu et al, 2020; Zhang et al, 2023). Furthermore, ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6) has been shown to regulate LBD16 activity, which contributes to downstream cell wall remodelling and promotes LR development under salinity stress (Zhang et al, 2023).…”

Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

“…Both LBD16 and XYLOGLUCAN ENDOTRANSGLUCOSYLASE 23 ( XTH23 )/ XTR6 play essential roles during salinity stress (Xu et al, 2020; Zhang et al, 2023). Furthermore, ZINC FINGER OF ARABIDOPSIS THALIANA 6 (ZAT6) has been shown to regulate LBD16 activity, which contributes to downstream cell wall remodelling and promotes LR development under salinity stress (Zhang et al, 2023). XTH23 / XTR6 , on the other hand, are involved in LR development via the BRI1‐EMS‐SUPPRESSOR 1 ( BES1 )‐dependent pathway and contribute to LR adaptation to salinity stress (Xu et al, 2020).…”

Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

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Serotonin: A frontline player in plant growth and stress responses

Mishra

Sarkar

2023

Physiologia Plantarum

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Serotonin is a well‐studied pineal hormone that functions as a neurotransmitter in mammals and is found in varying amounts in diverse plant species. By modulating gene and phytohormonal crosstalk, serotonin has a significant role in plant growth and stress response, including root, shoot, flowering, morphogenesis, and adaptability responses to numerous environmental signals. Despite its prevalence and importance in plant growth and development, its molecular action, regulation and signalling processes remain unknown. Here, we highlight the current knowledge of the role of serotonin‐mediated regulation of plant growth and stress response. We focus on serotonin and its regulatory connections with phytohormonal crosstalk and address their possible functions in coordinating diverse phytohormonal responses during distinct developmental phases, correlating with melatonin. Additionally, we have also discussed the possible role of microRNAs (miRNAs) in the regulation of serotonin biosynthesis. In summary, serotonin may act as a node molecule to coordinate the balance between plant growth and stress response, which may shed light on finding its key regulatory pathways for uncovering its mysterious molecular network.

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Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

Section: Serotonin In Plant Fitness and Stress Tolerancementioning

confidence: 99%

See 1 more Smart Citation

Serotonin: A frontline player in plant growth and stress responses

Mishra

Sarkar

2023

Physiologia Plantarum

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CYP79B2 and CYP79B3 contribute to root branching through production of the auxin precursor indole-3-acetonitrile

van Zelm,

Koevoets,

Meyer

et al. 2023

Preprint

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Lateral root placement, outgrowth and density are influenced by environmental changes, including salinity stress. CYP79B2 and B3 are two cytochrome P450 enzymes previously identified as required for root architecture remodeling in salt. They produce iAOx, a metabolite that can be converted into indole glucosinolates (IGs), camalexin and indole-3-acetic acid (IAA), a type of auxin. We report here that lateral root appearance, induced by an auxin maximum in the bending zone after gravistimulation, is delayed in the absence of CYP79B2/B3. This delay traces back to a decrease in early lateral root growth after emergence, taking place before lateral roots are macroscopically visible. We measured gene transcripts and abundance of metabolites in the iAOx pathway in root segments that are forming lateral roots. Genes involved in tryptophane and IG biosynthesis were upregulated incyp79b2/b3mutants, suggesting a transcriptional feedback-loop. Salt stress was found to increase the expression of genes involved in IAN biosynthesis, a precursor of both IAA and camalexin, in the root during lateral root formation. Moreover, salt increases the concentration of IAN in tissue forming lateral roots in a CYP79B2/B3 dependent manner, but these changes in IAN did not coincide with altered IAA levels. Both the reduction in lateral root density under salt and the delayed lateral root appearance incyp79b2/b3knock-out mutants can be complemented by exogenous application of IAN. Our results reveal a role for the iAOx pathway in regulating the timing of lateral root appearance, allowing the modulation of lateral root density under salt stress.

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Abscisic acid signaling gates salt-specific responses of plant roots

Lamers,

Zhang,

van Zelm

et al. 2023

Preprint

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Soil salinity presents a dual challenge for plants, involving both osmotic and ionic stress. In response, plants deploy distinct yet interconnected mechanisms to cope with these facets of salinity stress. In this investigation, we observed a substantial overlap in the salt (NaCl)-induced transcriptional responses of Arabidopsis roots with those triggered by osmotic stress or the plant stress hormone abscisic acid (ABA), as anticipated. Notably, a specific cluster of genes responded uniquely to sodium (Na+) ions. Surprisingly, expression of sodium-induced genes exhibited a negative correlation with the ABA response and preceded the activation of genes induced by the osmotic stress component of salt. Elevated exogenous ABA levels resulted in the complete abolition of sodium-induced responses. Consistently, ABA signalling mutants displayed prolonged sodium-induced gene expression, coupled with increased root cell damage under high salinity conditions. Moreover, ABA signalling mutants were unable to redirect root growth to avoid high sodium concentrations and failed to contain their root cell swelling in the presence of elevated salt levels.In summary, our findings unveil an unexpected and pivotal role for ABA signaling in mitigating cellular damage induced by salinity stress and modulating sodium-specific responses in plant roots.

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Root branching in salt requires auxin-independent modulation of LBD16 function

Cited by 3 publications

References 54 publications

Serotonin: A frontline player in plant growth and stress responses

Serotonin: A frontline player in plant growth and stress responses

CYP79B2 and CYP79B3 contribute to root branching through production of the auxin precursor indole-3-acetonitrile

Abscisic acid signaling gates salt-specific responses of plant roots

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