Local hemostatic effects of microcrystalline partially deacetylated chitin hydrochloride

Sugamori, Tsuyoshi; Iwase, Hideaki; Maeda, Mutsuhiro; Inoue, Yoshihisa; Kurosawa, Hisashi

doi:10.1002/(sici)1097-4636(200002)49:2<225::aid-jbm10>3.0.co;2-v

Cited by 24 publications

(13 citation statements)

References 8 publications

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“…Platelet activated by these stimulators releases alpha-granule constituents, such as PF4 and growth factors (12,13). Although chitosan has previously been reported to cause platelet activation (34)(35)(36)(37), the effect of differences in Mw and DDA has remained elusive. Therefore, basic studies using 13 types of chitosan with varying Mw and DDA were performed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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Abstract. Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that exhibits high platelet activation ability was investigated. A total of 13 chitosan samples with different molecular weight (Mw) and degree of deacetylation (DDA) were individually added to blood samples of rats and the amount of growth factors, albumin and fibrinogen in plasma was measured. To examine the influence of plasma activated by chitosan on the proliferation of fibroblasts and adipose tissue-derived stromal cells (ASCs), the plasma was added to the culture medium of human fibroblasts and adipose tissue-derived stromal cells. Chitosan with a DDA of >75% increased the release of platelet factor 4 into the plasma. The amount of growth factors released into the plasma and platelet activation varied depending on the Mw and DDA, while albumin and fibrinogen were hardly affected. The proliferation rate was highest when using plasma activated by chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da. These results suggested that the effectiveness of PRP therapy may be improved by using chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da.

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Section: Discussionmentioning

confidence: 99%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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“…In spite of a few challenges in preparing chitosan-based scaffolds due to its ionic characteristics, chitosan nanofibrous scaffolds were reported to offer better adhesion, proliferation and differentiation of keratinocytes, fibroblasts and endothelial cells, as well as enhanced vascularization and formation of granulation tissue compared to two dimensional (2D) films and 3D sponges of chitosan [8,247,248]. There are several different chitin/chitosan formulations reported for wound healing properties, such as water-soluble chitin (WSC) ointment [1], partially deacetylated chitin hydrochloride [249], chitin and silver nanoparticles/silver sulfadiazine/nano zinc oxide composites [250,251], phosphorylated chitin and chitosan, co-cultured keratinocyte and fibroblast cells on chitin/chitosan hydrogel membranes and scaffolds, antimicrobial films, sponges and hydrogels of chitosan [1], chitosan mesh membrane [252], chitosan films with antioxidant or thyme oil [1,253], and chitosan–Aloevera membranes [254]. …”

Section: Biomaterials and Nanobiomaterials Used For Several Scaffomentioning

confidence: 99%

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Chaudhari

Vig

Baganizi

et al. 2016

IJMS

454

288

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Over centuries, the field of regenerative skin tissue engineering has had several advancements to facilitate faster wound healing and thereby restoration of skin. Skin tissue regeneration is mainly based on the use of suitable scaffold matrices. There are several scaffold types, such as porous, fibrous, microsphere, hydrogel, composite and acellular, etc., with discrete advantages and disadvantages. These scaffolds are either made up of highly biocompatible natural biomaterials, such as collagen, chitosan, etc., or synthetic materials, such as polycaprolactone (PCL), and poly-ethylene-glycol (PEG), etc. Composite scaffolds, which are a combination of natural or synthetic biomaterials, are highly biocompatible with improved tensile strength for effective skin tissue regeneration. Appropriate knowledge of the properties, advantages and disadvantages of various biomaterials and scaffolds will accelerate the production of suitable scaffolds for skin tissue regeneration applications. At the same time, emphasis on some of the leading challenges in the field of skin tissue engineering, such as cell interaction with scaffolds, faster cellular proliferation/differentiation, and vascularization of engineered tissues, is inevitable. In this review, we discuss various types of scaffolding approaches and biomaterials used in the field of skin tissue engineering and more importantly their future prospects in skin tissue regeneration efforts.

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“…And IL-8 can inhibit collagenous synthesis [20], which might be helpful in decreasing the development of PVR. In addition, chitosan is a good hematischesis for capillary hemorrhage [21]; this has benefits for decreasing or eliminating chemotaxis of hema-factors to RPE, and thereby reducing or preventing the proliferative reaction of RPE. Meanwhile, chitosan, as well as inhibiting fibroblasts, might directly inhibit the proliferation of RPE.…”

Section: Figmentioning

confidence: 99%

Feasibility study of chitosan as intravitreous tamponade material

Yang

Wang

Gu³

et al. 2008

Graefes Arch Clin Exp Ophthalmol

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Chitosan intravitreous tamponade has no significant effect on the histology of the eye, doesn't cause intraocular pressure to fluctuate, and slightly increases inflammatory factors (IL-6, IL-8, NO) in comparison to the normal levels, but with no significant difference from the effects caused by sodium hyaluronate, which indicated chitosan might not lead to a clinically significant inflammatory response. It suggests that chitosan could be used as intravitreous tamponade material.

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Local hemostatic effects of microcrystalline partially deacetylated chitin hydrochloride

Cited by 24 publications

References 8 publications

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Feasibility study of chitosan as intravitreous tamponade material

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