Local Delivery of Vancomycin for the Prophylaxis of Prosthetic Device-Related Infections

Chilukuri, Dakshina Murthy; Shah, Jaymin

doi:10.1007/s11095-005-2497-7

Cited by 39 publications

(26 citation statements)

References 26 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On postoperative day (POD) 6, the rats in the VANCO and STIM + VANCO groups started receiving subcutaneous injections of vancomycin at 150 mg/ kg (V8138; Sigma-Aldrich, St Louis, MO, USA) twice a day. This dosing was derived from other experimental models [1,6,18,26,33] and pilot studies. On POD 7, all animals were anesthetized and the skin over the lateral cortex of the humerus was incised to expose the end of the Ti rod.…”

Section: Surgical Implantation and Infectionmentioning

confidence: 99%

Cathodic Electrical Stimulation Combined With Vancomycin Enhances Treatment of Methicillin-resistant Staphylococcus aureus Implant-associated Infections

Nodzo

Tobias

Hansen

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

Section: Surgical Implantation and Infectionmentioning

confidence: 99%

Cathodic Electrical Stimulation Combined With Vancomycin Enhances Treatment of Methicillin-resistant Staphylococcus aureus Implant-associated Infections

Nodzo

Tobias

Hansen

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…This impedes device integration into the surrounding tissue and protects bacteria from the host immune system and the action of systemic antibiotics. Thus, abiotic surface infections are recalcitrant to conventional systemic antimicrobial therapy based on susceptibility of planktonic cells (3,4). Development of potentially life-threatening systemic infections and device malfunction especially in critically-ill patients may necessitate risky and costly device removal or replacement procedures (5,6).…”

Section: Introductionmentioning

confidence: 99%

“…An active approach is based on drug/medical device combination products providing a localized bacterial inhibition effect by functionalizing the device biomaterial and/or coating with antibiotics, antiseptics, and metals (12). Localized delivery of high doses of antibiotics enhances efficacy, prevents systemic toxicity, and reduces bacterial resistance (3,13).…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the study was to evaluate a vancomycin (VCM)-eluting cosurfactant niosome formulation combining the potentials of Tween-hydrophilized vesicles as drug reservoir with bacterial adhesion impeding properties and those of VCM, recommended for the prophylaxis and treatment of implant-related staphylococcal infections (3,33). The attributes of the vesicles in terms of size, drug entrapment efficiency, Tween 40 content, VCM release characteristics, and physical stability were assessed with emphasis on vesicular retention of Tween 40 as a water soluble bilayer component, an aspect not clearly understood yet.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

et al. 2014

View full text Add to dashboard Cite

Abstract. A new vancomycin (VCM)-eluting mixed bilayer niosome formulation was evaluated for the control of staphylococcal colonization and biofilm formation on abiotic surfaces, a niosome application not explored to date. Cosurfactant niosomes were prepared using a Span 60/Tween 40/cholesterol blend (1: 1: 2). Tween 40, a polyethoxylated amphiphile, was included to enhance VCM entrapment and confer niosomal surface properties precluding bacterial adhesion. VCM-eluting niosomes showed good quality attributes including relatively high entrapment efficiency (∼50%), association of Tween 40 with vesicles in a constant proportion (∼87%), biphasic release profile suitable for inhibiting early bacterial colonization, and long-term stability at 4°C for a 12-month study period. Niosomes significantly enhanced VCM activity against planktonic bacteria of nine staphylococcal strains. Using microtiter plates as abiotic surface, VCM-eluting niosomes proved superior to VCM in inhibiting biofilm formation, eradicating surface-borne biofilms, inhibiting biofilm growth, and interfering with biofilm induction by VCM subminimal inhibitory concentrations. Data suggest dual functionality of cosurfactant VCM-eluting niosomes as passive colonization inhibiting barrier and active antimicrobial-controlled delivery system, two functions recognized in infection control of abiotic surfaces and medical devices.

show abstract

“…On postoperative Day (POD) 6, the rats in the VANCO, VANCO + 1STIM, and VANCO + 2STIM groups started receiving subcutaneous injections of vancomycin at 150 mg/kg (V8138; Sigma-Aldrich, St Louis, MO, USA) twice a day. This dosing was derived from other experimental models [1,4,15,21,25] and previous work in our laboratory [19]. On POD 7, all animals were anesthetized and the skin over the lateral cortex of the humerus was incised to expose the end of the Ti rod.…”

Section: Treatment Protocolmentioning

confidence: 99%

Cathodic Voltage-controlled Electrical Stimulation Plus Prolonged Vancomycin Reduce Bacterial Burden of a Titanium Implant-associated Infection in a Rodent Model

Nodzo

Tobias

Ahn

et al. 2016

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Cathodic voltage-controlled electrical stimulation (CVCES) of titanium implants, either alone or combined with a short course of vancomycin, has previously been shown to reduce the bone and implant bacterial burden in a rodent model of methicillin-resistant Staphylococcus aureus (MRSA) implant-associated infection (IAI). Clinically, the goal is to achieve complete eradication of the IAI; therefore, the rationale for the present study was to evaluate the antimicrobial effects of combining CVCES with prolonged antibiotic therapy with the goal of decreasing the colony-forming units (CFUs) to undetectable levels.Questions/purposes (1) In an animal MRSA IAI model, does combining CVCES with prolonged vancomycin therapy decrease bacteria burden on the implant and surrounding bone to undetectable levels? (2) When used with prolonged vancomycin therapy, are two CVCES treatments more effective than one? (3) What are the longer term histologic effects (inflammation and granulation tissue) of CVCES on the surrounding tissue? Methods Twenty adult male Long-Evans rats with surgically placed shoulder titanium implants were infected with a clinical strain of MRSA (NRS70). One week after infection, the rats were randomly divided into four groups of five: (1) VANCO: only vancomycin treatment (150 mg/ kg, subcutaneous, twice daily for 5 weeks); (2) VANCO + 1STIM: vancomycin treatment (same as the VANCO group) coupled with one CVCES treatment (À1.8 V for 1 hour on postoperative day [POD] 7); (3) VANCO + 2STIM: vancomycin treatment (same as the VANCO group) coupled with two CVCES treatments (À1.8 V for 1 hour on POD 7 and POD 21); or (4) CONT: no treatment. On POD 42, the implant, bone, and peripheral blood were collected for CFU enumeration and histological analysis, where we compared CFU/mL on the implants and bone among the groups. A pathologist, blinded to the experimental conditions, performed a semiquantitative analysis Clinical Orthopaedics and Related Research ®A Publication of The Association of Bone and Joint Surgeons® of inflammation and granulation tissue present in serial sections of the humeral head for animals in each experimental group. Results The VANCO + 1STIM decreased the implant bacterial burden (median = 0, range = 0-10 CFU/mL) when compared with CONT (median = 5.7 9 10 4 , range = 4.0 9 10 3 À8.0 9 10 5 CFU/mL; difference of medians = À5.6 9 10 4 ; p \ 0.001) and VANCO (median = 4.9 9 10 3 , range = 9.0 9 10 2 À2.1 9 10 4 CFU/mL; difference of medians = À4.9 9 10 3 ; p \ 0.001). The VANCO + 1STIM decreased the bone bacterial burden (median = 0, range = 0-0 CFU/mL) when compared with CONT (median = 1.3 9 10 2 , range = 0-9.4 9 10 2 CFU/ mL; difference of medians = À1.3 9 10 2 ; p \ 0.001) but was not different from VANCO (median = 0, range = 0-1.3 9 10 2 CFU/mL; difference of medians = 0; p = 0.210). The VANCO + 2STIM group had implant CFU (median = 0, range = 0-8.0 9 10 1 CFU/mL) and bone CFU (median = 0, range = 0-2.0 9 10 1 CFU/mL) that were not different from the VANCO + 1STIM treatment ...

show abstract

Local Delivery of Vancomycin for the Prophylaxis of Prosthetic Device-Related Infections

Abstract: The GMS implants as a delivery system for vancomycin were successful in preventing infection in all the animals compared to the IM and control groups demonstrating the efficacy of a local delivery system in a prosthetic device related biofilm infection model.

Cited by 39 publications

References 26 publications

Cathodic Electrical Stimulation Combined With Vancomycin Enhances Treatment of Methicillin-resistant Staphylococcus aureus Implant-associated Infections

Cathodic Electrical Stimulation Combined With Vancomycin Enhances Treatment of Methicillin-resistant Staphylococcus aureus Implant-associated Infections

Vancomycin-Eluting Niosomes: A New Approach to the Inhibition of Staphylococcal Biofilm on Abiotic Surfaces

Cathodic Voltage-controlled Electrical Stimulation Plus Prolonged Vancomycin Reduce Bacterial Burden of a Titanium Implant-associated Infection in a Rodent Model

Contact Info

Product

Resources

About