Local Application of Vancomycin for Prophylaxis of Graft Infection: Release of Vancomycin From Antibiotic-Bonded Dacron Grafts, Toxicity in Endothelial Cell Culture, and Efficacy Against Graft Infection in an Animal Model

Muhl, Elke; Gatermann, Sören; Iven, H.; Dendorfer, Andreas; Bruch, Hans–Peter

doi:10.1007/bf02001890

Cited by 21 publications

(12 citation statements)

References 34 publications

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“…However, a significantly higher LDH release and a higher grade of morphological cell damage in endothelial cell cultures were reported for teicoplanin when compared to vancomycin. This may indicate higher therapeutic safety for vancomycin in local therapy [28]. Our data revealed that the overall sustained release characteristics of vancomycin were superior to those of teicoplanin.…”

Section: Discussioncontrasting

confidence: 46%

Release of Vancomycin and Teicoplanin from a Plasticized and Resorbable Gelatin Sponge: in Vitro Investigation of a New Antibiotic Delivery System with Glycopeptides

et al. 2006

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Section: Discussioncontrasting

confidence: 46%

Release of Vancomycin and Teicoplanin from a Plasticized and Resorbable Gelatin Sponge: in Vitro Investigation of a New Antibiotic Delivery System with Glycopeptides

et al. 2006

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“…However, a significantly higher grade of morphological cell damage and a higher LDH release in endothelial cell cultures were seen for VM versus TN. This may point to higher therapeutic safety for TN in local drug delivery (21). The current study data showed that the overall sustained release characteristics of TN were higher than those of VM.…”

Section: Discussionmentioning

confidence: 53%

“…The major advantage of local application of antibiotics is producing high concentration in the infected sites without toxic effects due to systemic administration. Biodegradable antibiotic delivery systems are proven to have advantages over inert polymers (11,21,22). Once they are implanted, there is no need for a second surgical procedure for removal.…”

Section: Discussionmentioning

confidence: 99%

The Use of Natural Biopolymer of Chitosan as Biodegradable Beads for Local Antibiotic Delivery: Release Studies

Movaffagh

Ghodsi

Bazzaz

et al. 2013

Jundishapur J Nat Pharm Prod

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A B S T R A C T Background:Chitosan is a naturally occurring biopolymer which has been widely used in a variety of biomedical applications including local antibiotic delivery due to its excellent mechanical properties, biodegradability and biocompatibility. Beads are spherical, porous carriers which are prepared from various materials including chitosan. Objectives: The current study aimed to fabricate a new controlled delivery system for local anti-infective treatment and to study its release behavior. Materials and Methods: Twenty beads were prepared from 1% or 2% chitosan solutions and immersed in vancomycin (VM) or teicoplanin (TN) solutions. The antibiotic release kinetics was determined by linear regression analysis supposing first order kinetics. Results: Immersion for 3 h resulted in significant increase in the total TN release that differed from 0.5 h of immersion, except for the 1% beads immersed in VM. Increasing the chitosan concentration significantly increased the total release and antibiotic load of beads. The release of TN was more delayed compared to that of VM, which allowed a gradual release beyond 3 days. The half-life (mean ± SEM) of both types of TNcontaining beads was significantly extended for 3 h immersion in comparison to 0.5 h immersion (26.1 ± 5.9 vs 10.9 ± 1.0 and 17.0 ± 2.1 vs 5.1 ± 1.9; P < 0.001). However, neither increasing the chitosan concentration, nor immersion time did result in any significant increase in the release of VM. Conclusions:The current study demonstrated an improved control of TN release impregnated in beads. It can be concluded that chitosan beads might be considered as a novel carrier for TN delivery to infected bone for local anti-infective therapy.

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“…LDH concentration increased with rising vancomycin concentrations [28], but after 12-h incubation with vancomycin concentrations of 10 to 1000 μg/ml, the number of viable cells decreased slightly, and higher vancomycin concentrations of 10 000 μg/ml led to a cell survival rate of only 14.4% [28]. …”

Section: Discussionmentioning

confidence: 99%

Vascular Graft Impregnation with Antibiotics: The Influence of High Concentrations of Rifampin, Vancomycin, Daptomycin, and Bacteriophage Endolysin HY-133 on Viability of Vascular Cells

Herten

Idelevich²,

Sielker

et al. 2017

Med Sci Monit Basic Res

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BackgroundRifampin-soaked synthetic prosthetic grafts have been widely used for prevention or treatment of vascular graft infections (VGIs).This in vitro study investigated the effect of the antibiotics daptomycin and vancomycin and the new recombinant bacteriophage endolysin HY-133 on vascular cells, as potential alternatives compared to rifampin.Material/MethodsPrimary human ECs, vascular smooth muscle cells (vSMC), and fibroblasts were cultivated in 96-well plates and incubated with rifampin, daptomycin, vancomycin, and endolysin HY-133 for 24 h. Subsequently, after washing, cell viability was determined by measuring mitochondrial ATP concentration. Antibiotics were used in their corresponding minimum and maximum serum concentrations, in decimal multiples and in maximum soaking concentration. The experiments were performed in triplicate.ResultsThe 10-fold max serum concentrations of rifampin, daptomycin, and vancomycin did not influence viability of EC and vSMC (100 μg/ml, p>0.170). Higher concentrations of rifampin (>1 mg/ml) significantly (p<0.001) reduced cell viability of all cell types. For the other antibiotics, high concentrations (close to maximum soaking concentration) were most cytotoxic for EC and vSMC and fibroblasts (p<0.001). Endolysin did not display any cytotoxicity towards vascular cells.ConclusionsResults of this in vitro study show the high cytotoxicity of rifampin against vascular cells, and may re-initiate the discussion about the benefit of prophylactic pre-soaking in high concentrations of rifampin. Further studies are necessary to determine the influence of rifampin on the restoration of vessel functionality versus its prophylactic effect against VGIs. Future use of recombinant phage endolysins for alternative prophylactic strategies needs further investigations.

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Local Application of Vancomycin for Prophylaxis of Graft Infection: Release of Vancomycin From Antibiotic-Bonded Dacron Grafts, Toxicity in Endothelial Cell Culture, and Efficacy Against Graft Infection in an Animal Model

Cited by 21 publications

References 34 publications

Release of Vancomycin and Teicoplanin from a Plasticized and Resorbable Gelatin Sponge: in Vitro Investigation of a New Antibiotic Delivery System with Glycopeptides

Release of Vancomycin and Teicoplanin from a Plasticized and Resorbable Gelatin Sponge: in Vitro Investigation of a New Antibiotic Delivery System with Glycopeptides

The Use of Natural Biopolymer of Chitosan as Biodegradable Beads for Local Antibiotic Delivery: Release Studies

Vascular Graft Impregnation with Antibiotics: The Influence of High Concentrations of Rifampin, Vancomycin, Daptomycin, and Bacteriophage Endolysin HY-133 on Viability of Vascular Cells

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