Local anesthetic bupivacaine induced ovarian and prostate cancer apoptotic cell death and underlying mechanisms in vitro

Xuan, Wei; Zhao, Hailin; Hankin, James; Chen, Lin; Yao, Shanglong; Ma, Ding

doi:10.1038/srep26277

Cited by 80 publications

(75 citation statements)

References 47 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the reduction in cell viability measured by CellTiter-Blue ® for ropivacaine at 1,000 µM corresponds to a reduction in cell proliferation measured by PKH67 at the same concentration. Xuan et al reported similar findings in ovarian and prostate cancer cell lines (19). In their study, only bupivacaine at 1 mM decreased cell viability significantly, but not at lower concentrations.…”

Section: Discussionsupporting

confidence: 64%

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

et al. 2019

View full text Add to dashboard Cite

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose-dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5-1,000 µM). Cell viability was measured using CellTiter-Blue ® and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 µM) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 µM lidocaine in SW480 and by 1,000 µM ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti-proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.

show abstract

Section: Discussionsupporting

confidence: 64%

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In in vitro studies examining local anaesthetic effects on metastatic colon cancer cells, ropivacaine demonstrated inhibitory properties to these cells . Thus, the higher cancer specific mortality suggested in this follow‐up analysis was surprising …”

Section: Discussionmentioning

confidence: 71%

“…This has led to the changes in the way that perioperative providers care for patients undergoing cancer surgery including tailoring anaesthetic and surgical techniques to minimize the activation of systemic inflammatory pathways and hence reduce the stress response to the procedure . Studies examining the effects of local anaesthetics on ovarian, prostate and gastric cancer have all shown ‘anti‐cancer’ properties of the local anaesthetic . In in vitro studies examining local anaesthetic effects on metastatic colon cancer cells, ropivacaine demonstrated inhibitory properties to these cells .…”

Section: Discussionmentioning

confidence: 99%

Association between perioperative intraperitoneal local anaesthetic infusion and long‐term survival and cancer recurrence after colectomy: follow‐up analysis of a previous randomized controlled trial

MacFater

Xia

Barazanchi

et al. 2020

ANZ Journal of Surgery

View full text Add to dashboard Cite

Background: High concentrations of local anaesthetic have an anti-proliferative effect on colonic cancer in vitro. Intraperitoneal local anaesthetic (IPLA) has shown analgesic benefit and improved recovery in the perioperative setting. The long-term effects of IPLA in colon cancer resection have not been examined. This study aims to review the survival and oncological outcomes of a previously conducted trial that compared perioperative IPLA with placebo. Methods: Sixty patients underwent colonic resection for benign and malignant disease as part of a double-blinded, randomized, placebo-controlled study between September 2008 and November 2009. The IPLA group received instillation of intraperitoneal ropivacaine before dissection followed by a 3-day infusion. The placebo group was treated identically but with 0.9% saline solution. A follow-up analysis was conducted to evaluate overall survival, disease-free survival and recurrence specifically for patients undergoing resection for stages I-III colon cancer. Kaplan-Meier analysis was performed, and the log-rank test was used to evaluate difference in survival between groups. Results: Thirty-seven of the 60 patients had stages I-III colon cancer and were included in this analysis. Nineteen patients were in the placebo group. There was no significant difference in overall survival or all-cause mortality. There was a higher incidence of cancerspecific mortality in the local anaesthetic group (P < 0.046). Conclusion: It does not appear that IPLA is associated with a significant survival benefit in patients with colonic malignancy undergoing colectomy. Other studies are needed to analyse the long-term outcomes.© 2020 Royal Australasian College of Surgeons ANZ J Surg 90 (2020) 802-806ANZJSurg.com

show abstract

“…Although the immunosuppressive effect of opioids has been widely documented, some reports argue that opioids' immunomodulatory effects may be beneficial in the context of malignancy [16][17][18]. The effects of opioids on neoplasia progression likely depend on the extent of their analgesic action, counterbalancing their immunomodulatory effect by decreasing acute pain and attenuating the stress response.…”

Section: Mechanism Favoring Type Of Anesthesia and Cancer Recurrencementioning

confidence: 99%

“…Regional anesthesia also decreases the need for perioperative opioids, which are believed to have a protumoral effect [14,15]. In addition, studies on bupivacaine suggest that it has direct anti-neoplastic properties via activation of both intrinsic and extrinsic apoptotic pathways in ovarian cancer and the intrinsic pathway in prostate cancer [16]. Still, confounding evidence must be taken into account.…”

Section: The Effect Of Regional Anesthesia On Cancer Recurrencementioning

confidence: 99%

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Laudański¹,

Wei²,

Korley³

2016

J Anesth Clin Res

View full text Add to dashboard Cite

Alongside the known direct, short-term effects of anesthesia in general, there is emerging evidence of an immunomodulatory effect with specific anesthetics that may decrease patient's defences against malignant neoplastic growths. This effect is especially important in the setting of surgical management of neoplasms, which is often the best option for long-term survival in patients with solid neoplasm. Many studies have speculated on the best anesthetic technique to reduce the neoplasm recurrence and promote patient survival, however, we often neglect the sympathetic stress response to neoplasia and how anesthetics modulate this effect. In this review, we study the evidence as it pertains to anesthetic techniques and pain control, particularly general vs. regional anesthesia, and opioid analgesia. At this time there is not enough evidence to support that regional anesthesia has a more favorable outcome than general anesthesia, or that opioids should not be used in neoplasm-related pain management because of their potential pro-metastatic properties secondary to opioid-induced immunosuppression. Instead, the debate over anesthetic use should be centered on adequate pain control since overwhelming evidence have shown that pain-related stress reaction mediated via β-adrenergic activation, promotes neoplastic propagation and metastasis, hence decreasing survival rates.

show abstract

Local anesthetic bupivacaine induced ovarian and prostate cancer apoptotic cell death and underlying mechanisms in vitro

Cited by 80 publications

References 47 publications

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

Association between perioperative intraperitoneal local anaesthetic infusion and long‐term survival and cancer recurrence after colectomy: follow‐up analysis of a previous randomized controlled trial

Anesthetic Considerations for Management of Cancer Patients to Decrease Cancer Recurrence

Contact Info

Product

Resources

About