Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

Abstract

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose-dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5-1,000 µM). Cell viability was measured using CellTiter-Blue ® and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 µM) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 µM lidocaine in SW480 and by 1,000 µM ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti-proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.

Ropivacaine inhibits proliferation, invasion, migration and promotes apoptosis of papillary thyroid cancer cells via regulating ITGA2 expression

“…Compelling evidence has indicated that Ropivacaine can induce apoptosis of hepatocellular carcinoma cells via activation of caspase-3 expression (Wang et al, 2019). Accumulating evidence has highlighted the importance of Ropivacaine in inhibition of cancers, such as breast cancer, colon cancers and gastric cancer (Castelli et al, (2020) ;Siekmann, Tina, von Sydow, & Gupta, 2019;Yang et al, 2018). However, the role of Ropivacaine in the progression of PTC has not been clearly probed.…”

Lidocaine liposome modified with folic acid suppresses the proliferation and motility of glioma cells via targeting the PI3K/AKT pathway

“…Lidocaine is widely used as a local anesthetic. In addition to having anesthetic effects, lidocaine has also been reported to have inhibitory effects on tumors (25)(26)(27). A previous study demonstrated that lidocaine can inhibit vascular endothelial growth factor-A-induced angiogenesis, and therefore may be able to suppress cancer progression (28).…”

<p>Effects of Anesthesia on Postoperative Recurrence and Metastasis of Malignant Tumors</p>

“…Siekmann et al reported that clinical ropivacaine concentrations inhibited colon cancer cell proliferation in vitro in a dose-dependent manner. 87 Lirk et al reported that a similar lidocaine concentration caused DNA demethylation and activated tumor suppressor genes, especially in estrogen receptor-positive breast cancer cells. 88 Additionally, local anesthetic agents can promote tumor cell apoptosis.…”