Local and cardiorenal effects of periodontitis in nitric oxide-deficient hypertensive rats

Abstract

Objective In this study we have assessed the renal and cardiac consequences of ligature-induced periodontitis in both normotensive and nitric oxide (NO)-deficient (L-NAME-treated) hypertensive rats. Materials and methods Oral L-NAME (or water) treatment was started two weeks prior to induction of periodontitis. Rats were sacrificed 3, 7 or 14 days after ligature placement, and alveolar bone loss was evaluated radiographically. Thiobarbituric reactive species (TBARS; a lipid peroxidation index), protein nitrotyrosine (NT; a marker of protein nitration) and myeloperoxidase activity (MPO; a neutrophil marker) were determined in the heart and kidney. Results In NO-deficient hypertensive rats, periodontitis-induced alveolar bone loss was significantly diminished. In addition, periodontitis-induced cardiac NT elevation was completely prevented by L-NAME treatment. On the other hand L-NAME treatment enhanced MPO production in both heart and kidneys of rats with periodontitis. No changes due to periodontitis were observed in cardiac or renal TBARS content. Conclusions In addition to mediating alveolar bone loss, NO contributes to systemic effects of periodontitis in the heart and kidney.

Association of chronic periodontitis and psoriasis: periodontal status with severity of psoriasis

“…Further inducible nitric oxide synthase (iNOS), which upregulated due to TNF-a expression, is considered potential marker for psoriasis and CP (Gyurko et al, 2005;Wilson et al, 2007;Asarch et al, 2008;Racz and Prens, 2009). Gyurko et al (2005) have shown that iNOS-derived NO promotes bone resorption through osteoclast differentiation during bone development, as well as after bacterial infection in Porphyromonas gingivalis-induced periodontal disease in mice (Herrera et al, 2011) which can increase the susceptibility to the development of psoriasis or the severity of established psoriasis as reported by Davidovici et al (2010). Psoriatic immunopathogenesis consists of the complex interplay among innate and acquired immune cell types and among immune factors produced by dendritic cells, T cells, and keratinocytes in the psoriatic plaque (Lowes et al, 2007;Ouyang et al, 2008;Levine and Gottlieb, 2009).…”

“…Further inducible nitric oxide synthase (iNOS), which upregulated due to TNF‐ α expression, is considered potential marker for psoriasis and CP (Gyurko et al , ; Wilson et al , ; Asarch et al , ; Racz and Prens, ). Gyurko et al () have shown that iNOS‐derived NO promotes bone resorption through osteoclast differentiation during bone development, as well as after bacterial infection in Porphyromonas gingivalis ‐induced periodontal disease in mice (Herrera et al , ) which can increase the susceptibility to the development of psoriasis or the severity of established psoriasis as reported by Davidovici et al ().…”

Avaliação da ativação de células polimorfonucleares circulantes em ratos com periodontite induzida por ligadura.

“…É altamente prevalente e resulta na perda progressiva do tecido gengival, do ligamento periodontal e do suporte adjacente osso alveolar (Meng et al, 2007). Além disso, a periodontite tem sido associada com doenças sistêmicas, tais como complicações cardiovasculares (Campi et al, 2016;Herrera et al, 2011;Ryder, 2010), artrite reumatoide (Deas et al, 2003), resultados adversos da gravidez (Bender et al 2006),…”

“…A geração de ânion superóxido dos neutrófilos provenientes de animais do grupo Perio não é estatisticamente diferente daquela observada em animais do grupo (Herrera et al, 2011) seguindo um modelo bem estabelecido de inflamação periodontal induzido por ligadura em roedores (Lohinai et al, 1998;Rovin et al, 1966) e que se baseia em facilitar o aumento localizado da placa bacteriana pela ligadura (Spolidorio et al, 2002). Também foi observado diminuição do ganho ponderal de peso no grupo de ratos com ligadura avaliados no sétimo dia.…”

Papel dos receptores nucleares ativados por proliferadores de peroxissomos (PPAR) na periodontite induzida em ratos.

“…Chang et al(2001) A iNOS desempenha um importante papel tanto na sepse quanto na periodontite e tem na ativação do NF-kB e do AP-1 as principais vias de sinalização para a sua ativação. Demonstramos que tanto a inibição inespecífica das NOS pelo inibidor Nw-nitro L-arginina metilester (L-NAME) como a inibição específica da iNOS pelo mercaptoetilguanidina (aminoguanidina) diminui a perda óssea alveolar decorrente da periodontite em nosso modelo de periodontite induzida em ratos (Herrera et al,2010;Herrera et al,2011 (Wan et.al.,2007;Lecka-Czernik 2010;Lecka-Czernik and Suava 2006;Lazarenko et al, 2010) e por este motivo estudaremos os principais fatores transcricionais envolvidos na diferenciação e ativação de osteoblastos e osteoclastos. Na Tabela 2, observamos uma grande tendência de aumento da expressão gênica de RANKL nos animais tratados com o sal puro de rosiglitazona.…”