Local administration of aspirin improves osseointegration of hydroxyapatite-coated titanium implants in ovariectomized rats through activation of the Notch signaling pathway

Abstract

Osteoporosis-induced impaired bone regeneration would result in compromised osseointegration of hydroxyapatite-coated titanium and high rate of implant failure. Local administration of aspirin promotes osteoblast proliferation and inhibits osteoclast proliferation, and positively affects bone regeneration in osteoporotic condition. We hypothesized that reduced osteogenesis may account for poor osseointegration of hydroxyapatite-coated titanium which could be ameliorated by using local aspirin. The aim of this study was to confirm the effect of the local incorporation of aspirin into hydroxyapatite-coated titanium implants in the osteoporotic and normal condition. Twelve-week-old female Sprague–Dawley rats were used for this study. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into three groups: group Sham, group OVX and group OVX + ASP, and the rats from OVX and Sham received hydroxyapatite-coated implants and animals belong to group OVX + ASP received aspirin-hydroxyapatite-coated implants until death at 12 weeks, respectively. After 12-week healing period, local treatment with aspirin revealed improved osseointegration compared to OVX, with significant improvement of the bone area ratio and bone-to-implant contact in histomorphometry, the bone mass and trabecular architecture in micro-CT evaluation, and the maximal push-out force in push out test. Moreover, group OVX + ASP presented the strongest effect on Jagged1, Notch1, and Hes-1(P < 0.05). These results demonstrated that local administration of aspirin could enhance hydroxyapatite-coated titanium implant osseointegration in OVX rats by activation Wnt signaling pathway to improve implant fixation in osteoporotic bone.

Calcium fructoborate coating of titanium–hydroxyapatite implants by chemisorption deposition improves implant osseointegration in the femur of New Zealand White rabbit experimental model

“…Recently, Tao et al (2020) suggested that local incorporation of Acetylsalicylic Acid into HAp-coated Ti implants is useful to improve osseointegration by increasing bone formation around the implant through the activation of Notch signaling pathways, both in the osteoporotic and normal condition [ 31 ]. In addition, current animal studies demonstrate a possible improvement of osseointegration of the orthopedic implant in animal models from both systemic and local administration of Zoledronate [ 32 ].…”

Local administration with tauroursodeoxycholic acid could improve osseointegration of hydroxyapatite-coated titanium implants in ovariectomized rats

“…The rats were allowed for 12 weeks to conduct an osteoporosis as previous reports described. 22,23 The femurs of each five randomly selected OVX rats and the five shamoperated ones were harvested for bone mineral density (BMD) evaluation by micro computed tomography (Micro-CT) and hematoxylin(HE) staining to confirm the establishment of osteoporosis. Afterwards, all animals were randomly divided into three experimental groups: Sham group, OVX group and TUDCA group, the rats from OVX and Sham received HA implants and animals belonging to group TUDCA received TUDCA-HA implants as previously described.…”

“…The specimens were cut into 200-mm thick sections using a microtome(Leica Microsystems Ltd, Wetzlar, Germany) and were subsequently ground and polished to a thickness of 40-50 mm as previously described. 22,23 The femur specimens were stained in Von-Gieson for histopathological examination.…”

Silibinin can enhance the osseointegration of titanium rods in ovariectomized rats maintenance treatment with valproic acid

“…Following Micro-CT scanning, the femur specimens were dehydrated by alcohols and embedded in PMMA. The specimens were cut into 200-μm thick sections using a microtome(Leica Microsystems Ltd, Wetzlar, Germany) and were subsequently ground and polished to a thickness of 40-50 μm as previously described [21,22]. The femur specimens were stained in Von-Gieson for histopathological examination.…”

“…Total RNA was prepared and isolated using Trizol reagent (Invitrogen Corp., Carlsbad, CA, USA) as previously described [24,22]. Brie y, tissue samples around implant were homogenized using a mortar and pestle with liquid nitrogen, using 5ml of TRIzol per 50-100 mg of tissue.…”