LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2

Gong, Zhaojian; Zhang, Shanshan; Zeng, Zhaoyang; Wu, Hanjiang; Yang, Qian; Xiong, Fang; Shi, Lei; Yang, Jianbo; Zhang, Wenling; Zhou, Yanhong; Zeng, Yong; Li, Xiayu; Xiang, Bo; Peng, Shuping; Zhou, Ming; Li, Xiaoling; Tan, Ming; Li, Yong; Li, Guiyuan

doi:10.1371/journal.pone.0110674

Cited by 100 publications

(78 citation statements)

References 49 publications

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“…For example, an antisense lncRNA transcribed from the p15 tumor suppressor locus induces alterations to local heterochromatin and DNA methylation status, thereby regulating p15 expression, and may be associated with leukemia oncogenesis (29). TP53-regulated lncRNA (LOC401317) is directly regulated by p53 and has been demonstrated to exhibit antitumor effects in nasopharyngeal carcinoma cells (30). LOC401317 may inhibit cell cycle progression by upregulating p21, and decreasing cyclin D1 and cyclin E1 expression; it may also promote apoptosis through the induction of poly (ADP-ribose) polymerase (PARP) and caspase-3 cleavage (30).…”

Section: Discussionmentioning

confidence: 99%

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

et al. 2017

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Abstract. Long non-coding RNAs (lncRNAs) are emerging as a fundamental class of biological effect or molecules that perform pivotal functions in the regulation of the genome. With advances in bioinformatics and genomics, extensive identification and characterization of lncRNAs is now possible. They regulate cellular growth, differentiation and apoptosis. Dysregulation of lncRNAs has been associated with numerous types of human cancer. In the present study, the expression profile of differentially expressed genes (DEGs) and lncRNAs in gastric cancer (GC) samples and normal tissue samples was evaluated with bioinformatics. The biological functions of the predicted lncRNA TCONS_00068220 were focused on; the DEGs co-expressed with TCONS_00068220 were enriched in cancer-associated pathways. TCONS_00068220 was demonstrated to be upregulated in GC tissues and cell lines compared with normal controls. In addition, an increased rate of apoptosis was observed in NCI-N87 cells following transfection with small interfering RNA against TCONS_00068220. These data suggest that TCONS_00068220 may be associated with the pathogenesis of GC, and it may serve as a potential therapeutic target.

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Section: Discussionmentioning

confidence: 99%

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

et al. 2017

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“…LncRNAs compose a large set of RNA molecules that exceed 200 nt in length, completely lack or possess limited protein-coding capacity, and represent a substantial portion of the transcriptome [65][66][67][68][69][70][71][72]. LncRNAs widely regulate gene expression at the epigenetic, transcriptional, and post-transcriptional levels.…”

Section: Discussionmentioning

confidence: 99%

Integrating ChIP-sequencing and digital gene expression profiling to identify BRD7 downstream genes and construct their regulating network

Xiong

Zhou

et al. 2015

Mol Cell Biochem

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BRD7 is a single bromodomain-containing protein that functions as a subunit of the SWI/SNF chromatin-remodeling complex to regulate transcription. It also interacts with the well-known tumor suppressor protein p53 to trans-activate genes involved in cell cycle arrest. In this paper, we report an integrative analysis of genome-wide chromatin occupancy of BRD7 by chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) and digital gene expression (DGE) profiling by RNA-sequencing upon the overexpression of BRD7 in human cells. We localized 156 BRD7-binding peaks representing 184 genes by ChIP-sequencing, and most of these peaks were co-localized with histone modification sites. Four novel motifs were significantly represented in these BRD7-enriched regions. Ingenuity pathway analysis revealed that 22 of these BRD7 target genes were involved in a network regulating cell death and survival. DGE profiling identified 560 up-regulated genes and 1088 down-regulated genes regulated by BRD7. Using Gene Ontology and pathway analysis, we found significant enrichment of the cell cycle and apoptosis pathway genes. For the integrative analysis of the ChIP-seq and DEG data, we constructed a regulating network of BRD7 downstream genes, and this network suggests multiple feedback regulations of the pathways. Furthermore, we validated BIRC2, BIRC3, TXN2, and NOTCH1 genes as direct, functional BRD7 targets, which were involved in the cell cycle and apoptosis pathways. These results provide a genome-wide view of chromatin occupancy and the gene regulation network of the BRD7 signaling pathway.

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“…LOC401317 was highly induced by p53 transgene expression. Investigating its function in vitro and in vivo as a candidate tumor suppressor showed that LOC401317 was directly transcribed by p53 through a p53-binding site adjacent to its potential promoter region, and that LOC401317 inhibited cell cycle progression by increasing p21 and decreasing cyclin D1/E1 expressions, and promoted apoptosis through the induction of poly(ADP-ribose) polymerase and caspase-3 cleavage [60]. Nevertheless, current available information regarding this and other tumor suppressor lncRNAs involving in pathogenesis of NPC is limited, thus the true roles and functions of these lncRNAs in NPC still remains to be determined.…”

Section: Tumor Suppressor Lncrnasmentioning

confidence: 99%

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

Hann²

2018

Cell Physiol Biochem

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Nasopharyngeal carcinoma (NPC) is one of the most common cancers originating in the nasopharynx and occurring at high frequency in South-eastern Asia and North Africa. Long non-coding RNAs (lncRNAs) are a class of non-protein-coding RNA molecules and key regulators of developmental, physiological, and pathological processes in humans. Emerging studies have shown that lncRNAs play critical roles in tumorgenicity and cancer prognosis. With the development of deep sequencing analyses, an extensive amount of functional lncRNAs have been discovered in nasopharyngeal carcinoma tissues and cell lines. However, the roles and mechanisms of aberrantly expressed lncRNAs in the pathogenesis of NPC are not fully understood. In this review, we briefly illustrate the concept, identification, functional characterization, and summarize recent advancements of biological functions of lncRNAs with heterogeneous mechanistic characterization and their involvement in NPC. Then, we describe individual lncRNAs that have been associated with tumorgenesis, growth, invasion, cancer stem cell differentiation, metastasis, drug resistance and discuss the strategies of their therapeutic manipulation in NPC. We also review the emerging insights into the role of lncRNAs and their potential as biomarkers and therapeutic targets for novel treatment paradigms. Finally, we highlight the up-to-date of clinical information involving lncRNAs and future directions in the linking lncRNAs to potential gene therapies, and how modifications of lncRNAs can be exploited for prevention and treatment of NPC.

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LOC401317, a p53-Regulated Long Non-Coding RNA, Inhibits Cell Proliferation and Induces Apoptosis in the Nasopharyngeal Carcinoma Cell Line HNE2

Cited by 100 publications

References 49 publications

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

Downregulated long non‑coding RNA TCONS_00068220 upregulates apoptosis in gastric cancer cells

Integrating ChIP-sequencing and digital gene expression profiling to identify BRD7 downstream genes and construct their regulating network

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

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