Lobetyolin suppressed lung cancer in a mouse model by inhibiting epithelial-mesenchymal transition

Liu, Lu; Liu, Zhankui; Wu, X X; Zhu, Jun; Liu, Huan; Liu, Yang

doi:10.4081/ejh.2022.3423

Cited by 7 publications

(2 citation statements)

References 22 publications

(27 reference statements)

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“…Lobetyolin is one of the most important active components of CR which has good anticancer effects [ 40 , 41 , 42 ], exerts cytotoxic protection [ 43 ] and was also used to treat chronic kidney disease [ 44 ]. In the Chinese Pharmacopoeia (2020 edition), Lobetyolin was also employed as a distinguishing component to identify CR via thin-layer chromatography (TLC).…”

Section: Resultsmentioning

confidence: 99%

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

et al. 2023

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In China, Codonopsis Radix (CR) is frequently consumed both as food and medicine. Here, a comprehensive strategy based on fingerprinting and chemometric approaches was created to explore the influence of origins, storage time and kneading processing on the quality of CR. Firstly, high-performance liquid chromatography with diode array detection was used to obtain the fingerprints of 35 batches of CR from six different origins and 33 batches of CR from varying storage times or kneading procedures. Secondly, chemometric methods including similarity analysis (SA), principal component analysis (PCA), hierarchical clustering analysis (HCA), and two-way orthogonal partial least square with discriminant analysis (O2PLS—DA) were used to evaluate the differences of chemical components in CR so as to identify its source and reflect its quality. Moreover, 13 and 16 major compounds were identified as marker compounds for the discrimination of CR from different origins, storage time and kneading processing, respectively. Furthermore, the relative content of the marker components and the exact content of Lobetyolin were measured, indicating that the contents of these components vary significantly between various CR samples. Meanwhile, the chemical components of CR were identified using Mass spectrometry. According to the findings of our investigation, the quality of CR from Gansu was the best, followed by Shanxi and then Sichuan. The quality of CR from Chongqing and Guizhou was poor. At the same time, the quality of CR was the best when it was kneaded and stored for 0 years, indicating that the traditional kneading process of CR is of great significance. Conclusively, HPLC fingerprint in conjunction with chemical pattern recognition and component content determination can be employed to differentiate the raw materials of different CR samples. Additionally, it is also a reliable, comprehensive and prospective method for quality control and evaluation of CR.

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Section: Resultsmentioning

confidence: 99%

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

et al. 2023

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“…The lung tissue was fixed with 4% polyformaldehyde (Merck Life Science, 30525-89-4) and embedded in paraffin for further experiments. The tumor tissue was stored in a freezer at -80 °C and used to make frozen sections [15]. All experiments involving mice were approved by the Animal Ethics Committee of Quzhou People's Hospital.…”

Section: Methodsmentioning

confidence: 99%

The role of HMGA2 in activating the IGFBP2 expression to promote angiogenesis and LUAD metastasis via the PI3K/AKT/VEGFA signaling pathway

Qian,

Wang,

Liao

et al. 2024

neo

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is study investigates the molecular mechanism of HMGA2-mediated regulation of IGFBP2 expression in the PI3K/ AKT/VEGFA signaling pathway, which is involved in angiogenesis and LUAD metastasis. Target genes with prognostic implications for LUAD patients were selected using bioinformatics, and previously published literature was referenced to predict the molecular regulatory mechanisms. A549 cells were used for in vitro validation. Cell proliferation and viability were assessed using CCK-8 and EdU assays, while cell migration ability was evaluated using Transwell and wound healing assays. Changes in angiogenesis were examined using an angiogenesis assay. e targeted binding of HMGA2 with the IGFBP2 promoter was con rmed through dual luciferase reporter gene experiments and ChIP assays. In vivo validation was performed using a xenogra mouse model, and changes in angiogenesis and tumor metastasis were observed using western blot, immuno uorescence, and H&E staining. Bioinformatics analysis revealed that HMGA2 was one of the AAGs that di ered between normal individuals and LUAD patients and could serve as a critical mRNA for predicting LUAD prognosis. Results from in vitro experiments demonstrated that the expression of the HMGA2 gene was signi cantly upregulated in LUAD cell lines. rough mediating the expression of IGFBP2, the HMGA2 gene activated the PI3K/AKT/VEGFA signaling pathway, promoting the proliferation, migration, and angiogenesis of A549 cells. In vivo, animal experiments further con rmed that HMGA2 facilitated angiogenesis and the development and metastasis of LUAD through mediating IGFBP2 expression and activating the PI3K/AKT/VEGFA signaling pathway. HMGA2 promotes angiogenesis and healthy growth and metastasis of LUAD by activating the PI3K/AKT/VEGFA signaling pathway by mediating IGFBP2 expression.

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Lobetyolin inhibits cell proliferation and induces cell apoptosis by downregulating ASCT2 in gastric cancer

Cheng,

Zhai,

et al. 2023

Cytotechnology

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Lobetyolin suppressed lung cancer in a mouse model by inhibiting epithelial-mesenchymal transition

Cited by 7 publications

References 22 publications

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

Quality Assessment and Classification of Codonopsis Radix Based on Fingerprints and Chemometrics

The role of HMGA2 in activating the IGFBP2 expression to promote angiogenesis and LUAD metastasis via the PI3K/AKT/VEGFA signaling pathway

Lobetyolin inhibits cell proliferation and induces cell apoptosis by downregulating ASCT2 in gastric cancer

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