Loading of preformed liposomes with high trapping efficiency by detergent-induced formation of transient membrane holes

Schubert, Rolf; Wolburg, Hartwig; Schmidt, Karl‐Heinz; Roth, H. J.

doi:10.1016/0009-3084(91)90118-u

Cited by 42 publications

(18 citation statements)

References 20 publications

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“…In contrast to calcein, the release of FITC-dextran (MW 19,500) was less than 10% in 40% ethanol. This shows that the loss of the permeability barrier is MW dependent, as has also been reported for detergents such as octylglucoside and sodium cholate (Almog et al, 1990;Schubert et al, 1991). The liposomes maintained their morphology in the presence of 40% ethanol.…”

Section: Effect Of Ethanol On Liposome Stabilitysupporting

confidence: 79%

Spontaneous Entrapment of Polynucleotides upon Electrostatic Interaction with Ethanol-Destabilized Cationic Liposomes

Maurer

Wong

Stark

et al. 2001

Biophysical Journal

195

146

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This study describes the effect of ethanol and the presence of poly(ethylene) glycol (PEG) lipids on the interaction of nucleotide-based polyelectrolytes with cationic liposomes. It is shown that preformed large unilamellar vesicles (LUVs) containing a cationic lipid and a PEG coating can be induced to entrap polynucleotides such as antisense oligonucleotides and plasmid DNA in the presence of ethanol. The interaction of the cationic liposomes with the polynucleotides leads to the formation of multilamellar liposomes ranging in size from 70 to 120 nm, only slightly bigger than the parent LUVs from which they originated. The degree of lamellarity as well as the size and polydispersity of the liposomes formed increases with increasing polynucleotide-to-lipid ratio. A direct correlation between the entrapment efficiency and the membrane-destabilizing effect of ethanol was observed. Although the morphology of the liposomes is still preserved at the ethanol concentrations used for entrapment (25-40%, v/v), entrapped low-molecular-weight solutes leak rapidly. In addition, lipids can flip-flop across the membrane and exchange rapidly between liposomes. Furthermore, there are indications that the interaction of the polynucleotides with the cationic liposomes in ethanol leads to formation of polynucleotide-cationic lipid domains, which act as adhesion points between liposomes. It is suggested that the spreading of this contact area leads to expulsion of PEG-ceramide and triggers processes that result in the formation of multilamellar systems with internalized polynucleotides. The high entrapment efficiencies achieved at high polyelectrolyte-to-lipid ratios and the small size and neutral character of these novel liposomal systems are of utility for liposomal delivery of macromolecular drugs.

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Section: Effect Of Ethanol On Liposome Stabilitysupporting

confidence: 79%

Spontaneous Entrapment of Polynucleotides upon Electrostatic Interaction with Ethanol-Destabilized Cationic Liposomes

Maurer

Wong

Stark

et al. 2001

Biophysical Journal

195

146

View full text Add to dashboard Cite

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“…3b, only a weak leakage was observed after 20 min. Then we added to the vesicle suspension a detergent bearing a large head group following the strategy evoked in the Introduction [32]. The study was restricted to Brij 76 (C 18 OE 10 ) which induced the desired phenomenon of permeation under shearing.…”

Section: Shear-induced Leakage In Epc Vesiclesmentioning

confidence: 99%

Shear-induced permeation and fusion of lipid vesicles

Bernard

Guedeau-Boudeville

Marchi-Artzner

et al. 2005

Journal of Colloid and Interface Science

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“…This method is known as 'detergent-induced liposome loading' (DILL). 6 Although their biocompatibility and biodegradability make them safe and efficacious vehicles for medical applications, there still remain several problems to be overcome, one of such problems being the stability of lipid vesicles in blood and in the gastrointestinal tract. 7 The stability of liposomes in the gastrointestinal tract is largely affected by the bile salts; hence a proper understanding of the action of bile salts on liposomes is indispensable.…”

Section: Introductionmentioning

confidence: 99%

Effect of sodium deoxycholate and sodium cholate on DPPC vesicles: A fluorescence anisotropy study with diphenylhexatriene

Subuddhi

Mishra

2007

J Chem Sci

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Effects of two bile salts, namely sodium deoxycholate (NaDC) and sodium cholate (NaC), on DPPC small unilamellar vesicles have been investigated using the steady-state fluorescence anisotropy (r ss ) of diphenylhexatriene (DPH) as a tool. It was found that the variation of r ss is sensitive enough to monitor different stages of interaction of bile salts with DPPC vesicles. NaDC induced significant changes in the membrane well below its CMC (6 mM). Even at 4 mM, which is still lower than the CMC, the phospholipids were completely solubilised by the NaDC micelles. The effect of NaC on DPPC vesicles, however, was much less significant, especially in the sub-micellar concentration regime. Being more hydrophilic NaC does not interact with the membrane efficiently. Complete solubilisation of phospholipids took place only when the concentration of NaC was above its CMC (16 mM). The experiments also showed that the bile salt-induced changes of vesicle structure were strongly dependent on the concentration of the bile salt and not on the molar ratio of lipid and bile salt.

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Loading of preformed liposomes with high trapping efficiency by detergent-induced formation of transient membrane holes

Cited by 42 publications

References 20 publications

Spontaneous Entrapment of Polynucleotides upon Electrostatic Interaction with Ethanol-Destabilized Cationic Liposomes

Spontaneous Entrapment of Polynucleotides upon Electrostatic Interaction with Ethanol-Destabilized Cationic Liposomes

Shear-induced permeation and fusion of lipid vesicles

Effect of sodium deoxycholate and sodium cholate on DPPC vesicles: A fluorescence anisotropy study with diphenylhexatriene

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