Shear-induced permeation and fusion of lipid vesicles

Bernard, Anne-Laure; Guedeau-Boudeville, Marie-Alice; Marchi-Artzner, Valérie; Gulik-Krzywicki, T.; Meglio, Jean-Marc Di; Jullien, Ludovic

doi:10.1016/j.jcis.2004.12.019

Cited by 56 publications

(64 citation statements)

References 29 publications

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“…In ionic surfactant systems with oppositely charged strong binding counter-ions (22)(23)(24), when the shear effect on the aggregate structure was taken into account in the deformation energy of a free bilayer (25) through a shear-dependent contribution of spontaneous curvature C o arising from unbinding of counter-ions, the resultant shear diagram obtained by minimizing the free energy could predict a shear-induced vesicle-to-micelle transition. Similarly, in mixed lipid/surfactant systems, shear-induced partial segregation within the bilayer of the initially evenly distributed amphiphiles is believed to give rise to a change in spontaneous curvature and the membrane line tension leading to pore formation (26). Our study suggests that an analogous phenomenon of shear-induced modification of spontaneous curvature can drive phase transitions in concentrated bilayerforming mesophases.…”

Section: Ii) Transitions Occurring Well Abovementioning

confidence: 58%

Reversible shear-induced crystallization above equilibrium freezing temperature in a lyotropic surfactant system

Rathee

Krishnaswamy

Pal

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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We demonstrate a unique shear-induced crystallization phenomenon above the equilibrium freezing temperature ðT o K Þ in weakly swollen isotropic ðL i Þ and lamellar ðL α Þ mesophases with bilayers formed in a cationic-anionic mixed surfactant system. Synchrotron rheological X-ray diffraction study reveals the crystallization transition to be reversible under shear (i.e., on stopping the shear, the nonequilibrium crystalline phase L c melts back to the equilibrium mesophase). This is different from the shear-driven crystallization below T o K , which is irreversible. Rheological optical observations show that the growth of the crystalline phase occurs through a preordering of the L i phase to an L α phase induced by shear flow, before the nucleation of the L c phase. Shear diagram of the L i phase constructed in the parameter space of shear rate ð_ γÞ vs. temperature exhibits L i → L c and L i → L α transitions above the equilibrium crystallization temperature ðT o K Þ, in addition to the irreversible shear-driven nucleation of L c in the L i phase below T o K . In addition to revealing a unique class of nonequilibrium phase transition, the present study urges a unique approach toward understanding shear-induced phenomena in concentrated mesophases of mixed amphiphilic systems.shear-induced phase separation | strongly binding counterions | coagels S hear is known to assist crystallization below the equilibrium freezing temperature in complex fluids like colloidal glasses (1, 2), dense granular suspensions (3), polymer melts (4, 5), micellar solutions of block copolymers (6), and multicomponent surfactant systems (7,8). Shear-driven crystallization is equally relevant for simple fluids like bulk metallic glasses (9), molecular liquids (10), and atomic systems (11). The general understanding is that shear lowers the energy barrier for nucleation and accelerates the growth of a stable crystalline phase from a metastable, amorphous/ isotropic solution at Peclet number Pe = σa 3 kBT > 1, where σ is the shear stress, a is the characteristic length scale, and k B T is the thermal energy (12). The crystalline phase primarily induced by the effect of flow on the internal structure and ordering of the constituents does not revert to the starting fluid state when the imposed shear is removed, indicating that the phenomenon is not a dynamic phase transition. In the present study, we report a unique phenomenon, where under steady shear, crystallization occurs above the equilibrium crystallization temperature ðT o K Þ in an isotropic mesophase ðL i Þ consisting of bilayers formed in a lyotropic surfactant system. Notably, above T o K , when the imposed shear is removed, the crystalline phase melts back to the starting L i phase.The studies were carried out in a cationic-anionic mixed surfactant system formed by SDS and the strong binding counter-ion paratoluene hydrochloride (PTHC) in water. At equilibrium, the organic counter-ion PTHC has the tendency to remain at the micelle-water interface, decreasing the spontaneous curvat...

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Section: Ii) Transitions Occurring Well Abovementioning

confidence: 58%

Reversible shear-induced crystallization above equilibrium freezing temperature in a lyotropic surfactant system

Rathee

Krishnaswamy

Pal

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…That is why they are interesting as a substitute for other surfactants potentially damaging to the environment [9]. It has been already shown that shearing induces fusion and leakage of vesicles when the vesicles are formed of a lipid and a surfactant bearing a large head-group [10]. In such a way it is possible to control membrane permeability in free unilamellar lipid vesicles using shearing and a surfactant with a large head-group.…”

Section: Introductionmentioning

confidence: 99%

The effect of octyl glucoside on rheological behavior of diluted and concentrated lamellar phases

Granizo

Alvarez

Valiente

2006

Journal of Colloid and Interface Science

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“…In general, vesicle fusion is mediated by proteins, 3 but it can also be achieved through the use of multivalent ions, 4 polymers, 5,6 or surfactants. 7 Without the help of proteins or ions, inducing vesicle fusion is difficult because lipid vesicles have extremely low membrane tension at equilibrium 8 and large bilayer poration energy, which is on the order of 13k B T. 9 However, in the presence of a significant strain or shear, vesicle fusion can also be accomplished without the help of additional mediators. 10,11 Vesicle fusion typically occurs on a submillisecond time scale 12,13 or shorter.…”

Section: ■ Introductionmentioning

confidence: 99%

Flow-Driven Rapid Vesicle Fusion via Vortex Trapping

2015

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Fusion between suspended lipid vesicles is difficult to achieve without membrane proteins or ions because the vesicles have extremely low equilibrium membrane tension and high poration energy. Nonetheless, vesicle fusion in the absence of mediators can also be achieved by mechanical forcing that is strong enough to induce membrane poration. Here, we employ a strong fluid shear stress to achieve vesicle fusion. By utilizing a unique vortex formation phenomenon in branched channels as a platform for capturing, stressing, and fusing the lipid vesicles, we directly visualize using high-speed imaging the vesicle fusion events, induced solely by shear, on the time scale of submilliseconds. We show that a large vesicle with a size of up to ∼10 μm can be achieved by the fusion of nanoscale vesicles. This technique has the potential to be utilized as a fast and simple way to produce giant unilamellar vesicles and to serve as a platform for visualizing vesicle interactions and fusions in the presence of shear.

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Shear-induced permeation and fusion of lipid vesicles

Cited by 56 publications

References 29 publications

Reversible shear-induced crystallization above equilibrium freezing temperature in a lyotropic surfactant system

Reversible shear-induced crystallization above equilibrium freezing temperature in a lyotropic surfactant system

The effect of octyl glucoside on rheological behavior of diluted and concentrated lamellar phases

Flow-Driven Rapid Vesicle Fusion via Vortex Trapping

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