Loading neural stem cells on hydrogel scaffold improves cell retention rate and promotes functional recovery in traumatic brain injury

Chen, Tiange; Xia, Yuguo; Zhang, Jintao; Xu, Tao; Yi, Yan; Chen, Jianwei; Liu, Ziyuan; Yang, Liting; Chen, Siming; Zhou, Xiaoxi; Chen, Xin; Wu, Haiyu; Liu, Jinfang

doi:10.1016/j.mtbio.2023.100606

Cited by 13 publications

(8 citation statements)

References 66 publications

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“…Nanohybrid hydrogels containing sufated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) are able to mimic extracellular matrices and contain a variety of bioactive factors to improve the implantation rate of neural SCs, while enabling cellular responses after central nervous system injury ( Jian et al, 2018 ). Gelatin methacrylate (GelMA)/sodium alginate (Alg) (GelMA/Alg) hydrogels also contribute to the reduction of cellular damage after the implantation of neural SCs ( Chen et al, 2023 ). Hydrogels of different compositions have played essential roles in cardiac infarction, skin regeneration, liver regeneration, etc., ( Mardpour et al, 2019 ; Ke et al, 2020 ; Gong et al, 2022 ).…”

Section: The Technical Route To Sc Therapymentioning

confidence: 99%

Enhancing regenerative medicine: the crucial role of stem cell therapy

Wang,

Deng,

Wang

et al. 2024

Front. Neurosci.

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Stem cells offer new therapeutic avenues for the repair and replacement of damaged tissues and organs owing to their self-renewal and multipotent differentiation capabilities. In this paper, we conduct a systematic review of the characteristics of various types of stem cells and offer insights into their potential applications in both cellular and cell-free therapies. In addition, we provide a comprehensive summary of the technical routes of stem cell therapy and discuss in detail current challenges, including safety issues and differentiation control. Although some issues remain, stem cell therapy demonstrates excellent potential in the field of regenerative medicine and provides novel tactics and methodologies for managing a wider spectrum of illnesses and traumas.

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Section: The Technical Route To Sc Therapymentioning

confidence: 99%

Enhancing regenerative medicine: the crucial role of stem cell therapy

Wang,

Deng,

Wang

et al. 2024

Front. Neurosci.

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“…In a recent study by Chen et al, a 3D-printed scaffold was designed using sodium alginate and gelatin methacrylate [ 119 ]. Their investigation into the hydrogel’s modulus revealed that lower alginate ratios in the hydrogel composition led to reduced deformation when subjected to compression forces, simulating the brain tissue environment.…”

Section: Cell Encapsulation Techniquesmentioning

confidence: 99%

Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Kavand,

Noverraz,

Gerber-Lemaire

2024

Pharmaceutics

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With its exceptional biocompatibility, alginate emerged as a highly promising biomaterial for a large range of applications in regenerative medicine. Whether in the form of microparticles, injectable hydrogels, rigid scaffolds, or bioinks, alginate provides a versatile platform for encapsulating cells and fostering an optimal environment to enhance cell viability. This review aims to highlight recent studies utilizing alginate in diverse formulations for cell transplantation, offering insights into its efficacy in treating various diseases and injuries within the field of regenerative medicine.

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“…Gene manipulation techniques, such as the cellular reprogramming of NSCs via the regulation of c-Myc, high-mobility group box 1 (HMGB1), interleukin 6, mutated estrogen receptor transgene, or PEP-1-SOD1, have been shown to promote proliferation, differentiation into neuronal lineage, and migration [ 7 – 10 ]. Chen et al conducted a study in which NSCs were cultured in an alginate scaffold; this method resulted in increased survival rates [ 11 ]. Nevertheless, the overall efficacy of these techniques remains limited, particularly following transplantation.…”

Section: Introductionmentioning

confidence: 99%

Linc-NSC affects cell differentiation, apoptosis and proliferation in mouse neural stem cells and embryonic stem cells in vitro and in vivo

Guo,

Zou,

Qiu

et al. 2024

Cell. Mol. Life Sci.

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Background Stem cell therapy is a promising therapeutic strategy. In a previous study, we evaluated tumorigenicity by the stereotactic transplantation of neural stem cells (NSCs) and embryonic stem cells (ESCs) from experimental mice. Twenty-eight days later, there was no evidence of tumor formation or long-term engraftment in the NSCs transplantation group. In contrast, the transplantation of ESCs caused tumor formation; this was due to their high proliferative capacity. Based on transcriptome sequencing, we found that a long intergenic non-coding RNA (named linc-NSC) with unknown structure and function was expressed at 1100-fold higher levels in NSCs than in ESCs. This finding suggested that linc-NSC is negatively correlated with stem cell pluripotency and tumor development, but positively correlated with neurogenesis. In the present study, we investigated the specific role of linc-NSC in NSCs/ESCs in tumor formation and neurogenesis. Methods Whole transcriptome profiling by RNA sequencing and bioinformatics was used to predict lncRNAs that are widely associated with enhanced tumorigenicity. The expression of linc-NSC was assessed by quantitative real-time PCR. We also performed a number of in vitro methods, including cell proliferation assays, differentiation assays, immunofluorescence assays, flow cytometry, along with in vivo survival and immunofluorescence assays to investigate the impacts of linc-NSC on tumor formation and neurogenesis in NSCs and ESCs. Results Following the knockdown of linc-NSC in NSCs, NSCs cultured in vitro and those transplanted into the cortex of mice showed stronger survival ability (P < 0.0001), enhanced proliferation(P < 0.001), and reduced apoptosis (P < 0.05); the opposite results were observed when linc-NSC was overexpressed in ESCs. Furthermore, the overexpression of linc-NSC in ECSs induced enhanced apoptosis (P < 0.001) and differentiation (P < 0.01), inhibited tumorigenesis (P < 0.05) in vivo, and led to a reduction in tumor weight (P < 0.0001). Conclusions Our analyses demonstrated that linc-NSC, a promising gene-edited target, may promote the differentiation of mouse NSCs and inhibit tumorigenesis in mouse ESCs. The knockdown of linc-NSC inhibited the apoptosis in NSCs both in vitro and in vivo, and prevented tumor formation, revealing a new dimension into the effect of lncRNA on low survival NSCs and providing a prospective gene manipulation target prior to transplantation. In parallel, the overexpression of linc-NSC induced apoptosis in ESCs both in vitro and in vivo and attenuated the tumorigenicity of ESCs in vivo, but did not completely prevent tumor formation.

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Loading neural stem cells on hydrogel scaffold improves cell retention rate and promotes functional recovery in traumatic brain injury

Cited by 13 publications

References 66 publications

Enhancing regenerative medicine: the crucial role of stem cell therapy

Enhancing regenerative medicine: the crucial role of stem cell therapy

Recent Advances in Alginate-Based Hydrogels for Cell Transplantation Applications

Linc-NSC affects cell differentiation, apoptosis and proliferation in mouse neural stem cells and embryonic stem cells in vitro and in vivo

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