“…Cholesterol depletion increases the adhesion of actin cytoskeleton to the plasma membrane (Khatibzadeh, Nima, Gupta, 2012), which may negatively impact actin dynamics. Whereas osmotic swelling increases the in-plane membrane tension without affecting the function of actin machinery (Dai et al ., 1998; Boulant et al ., 2012; Ferguson et al ., 2017; Kaplan et al ., 2022). Considering the fact that actin machinery becomes indispensable for internalization of clathrin-coated vesicles under high mechanical load (Boulant et al ., 2011; Ferguson et al ., 2017), we propose that splitting and internalization of GCPs under high membrane tension is also dependent on the forces provided by actin polymerization.…”