lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene

Zhu, Ruirui; Xue, Feng; Wei, Yutong; Guo, Duo; Li, Jiaojiao; Liu, Qingyou; Jiang, Jianrong; Shi, Deshun; Huang, Jieping

doi:10.3389/fgene.2021.626158

Cited by 9 publications

(10 citation statements)

References 41 publications

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“…lncRNAs are involved in a variety of bioregulatory processes, including muscle growth, fat deposition, and lipid metabolism [50]. For example, lncSAMM50 [51], lncRNA 1 [52] enhances adipocytes differentiation. We showed that interfering with LincRNA-MSTRG.673.2 inhibited intramuscular fat deposition.…”

Section: Disscussionmentioning

confidence: 99%

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

Zhu#,

Zhang,

et al. 2024

Preprint

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Background Intramuscular fat content is positively correlated with meat flavor and juiciness. Increasing the intramuscular fat (IMF) content of chickens while increasing their growth rate has become a hot topic in molecular breeding.The group's previous studies showed that miR-128-3p inhibited chicken intramuscular adipocyte differentiation and lipogenesis. However, the regulatory mechanism of miR-128-3p in intramuscular preadipocytes is currently unknown. In this study, we investigated the mechanism of miR-128-3p regulation of chicken intramuscular adipocyte differentiation and deposition. Methods RNA-seq was performed to screen for long non-coding RNAs (lncRNAs) that bind to miR-128-3p. Dual luciferase reporter system was used to verify the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2; nucleoplasmic localization analysis and fluorescence in situ hybridization were used to investigate the localization of LincRNA-MSTRG.673.2 in the cells; a series of experiments such as Q-PCR, Oil O Red staining and triglyceride assay were used to explore the effect of interference with LincRNA-MSTRG.673.2 on the differentiation of intramuscular preadipocytes; co-transfection experiments were used to validate the regulatory patterns of miR-128-3p and LincRNA-MSTRG.673.2 in intramuscular adipocytes. Results Transcriptome data analysis of differential LincRNAs indicated that, compared to the NC group, the mimics-treated group had 17 significantly differentially expressed LincRNAs (P < 0.05), including 6 upregulated and 11 downregulated ones; the inhibitor-treated group had 17 differentially expressed LincRNAs (P < 0.05), including 8 upregulated and 9 downregulated ones; and 24 differentially expressed LincRNAs (P < 0.05) were observed when comparing the mimics-treated group to the inhibitor-treated group, with 14 upregulated and 10 downregulated ones. Functional enrichment analysis revealed that DELincRNAs from the overexpression group (M group) and interference group (SI group) were involved in negative regulation of metabolic processes, response to steroid hormones, regulation of actin cytoskeleton. Furthermore, target gene prediction analysis showed that miR-128-3p can target many of the DELincRNAs, such as LincRNA-MSTRG.673.2, LincRNA-MSTRG.39.2, LincRNA-MSTRG.39.3, and LincRNA-MSTRG.14270.2. LincRNA-MSTRG.673.2 was predominantly expressed in cytoplasm of intramuscular adipocytes. Dual luciferase reporter identified the targeting relationship between miR-128-3p and LincRNA-MSTRG.673.2. The results of subsequent functional assays demonstrated that Interfering with MSTRG.673.2 has been shown to inhibit lipid deposition in intramuscular preadipocytes. Transfection experiments have shown that LincR-MSTRG.673.2 can affect the expression of miR-128-3p. Conclusion This study found that LincRNA-MSTRG.673.2 promoted chicken intramuscular adipocytes differentiation by down regulating miR-128-3p. The results are noteworthy for improving chicken meat quality, molecular breeding, and lipid metabolism research.

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Section: Disscussionmentioning

confidence: 99%

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

Zhu#,

Zhang,

et al. 2024

Preprint

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“…EGFP uorescence was used to monitor infection e ciency. Adenoviral transductions were performed according to laboratory protocols described in previous studies [13].…”

Section: Adenovirus Production and Transductionmentioning

confidence: 99%

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

Zhu

Yang

et al. 2023

Preprint

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Intramuscular fat (IMF) is a crucial determinant of meat quality and is influenced by various regulatory factors. Despite the growing recognition of the important role of long noncoding RNAs (lncRNAs) in IMF deposition, the mechanisms underlying buffalo IMF deposition remain poorly understood. In this study, we identified and characterized a novel lncRNA, lncFABP4, which was transcribed from the antisense strand of FABP4. Moreover, lncFABP4 significantly increased intramuscular preadipocyte differentiation, as indicated by an increase in the expression of the adipogenic markers peroxisome proliferator-activated receptor gamma (PPARG), CCAAT enhancer binding protein alpha (C/EBPα), and fatty acid binding protein 4 (FABP4). Mechanistically, lncFABP4 was found to have the potential to regulate downstream gene expression by forming transcriptional complexes with the FABP4 protein. These findings contribute to a further understanding of the intricate mechanisms through which lncRNAs modulate intramuscular adipogenesis in buffaloes.

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“…In addition, Ctcflos [124] operate by variable shearing and thus shearing out shorter Prdm16 isoform transcripts which participate in promoting white fat browning. Some lncRNAs promote or inhibit the expression of downstream genes such as adipogenic differentiation, but the molecular mechanism underlying the regulation of related downstream genes, such as PVT1, lncSAMM50, lncAK079912, lnc 2310069B03Rik, HOTAIR, Dreh, LIPE-AS1, and HoxA11-AS1 are not well understood [125][126][127][128][129][130][131][132][133].…”

Section: Regulates Downstream Genesmentioning

confidence: 99%

LncRNA-Mediated Adipogenesis in Different Adipocytes

Zhang

et al. 2022

IJMS

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Long-chain noncoding RNAs (lncRNAs) are RNAs that do not code for proteins, widely present in eukaryotes. They regulate gene expression at multiple levels through different mechanisms at epigenetic, transcription, translation, and the maturation of mRNA transcripts or regulation of the chromatin structure, and compete with microRNAs for binding to endogenous RNA. Adipose tissue is a large and endocrine-rich functional tissue in mammals. Excessive accumulation of white adipose tissue in mammals can cause metabolic diseases. However, unlike white fat, brown and beige fats release energy as heat. In recent years, many lncRNAs associated with adipogenesis have been reported. The molecular mechanisms of how lncRNAs regulate adipogenesis are continually investigated. In this review, we discuss the classification of lncRNAs according to their transcriptional location. lncRNAs that participate in the adipogenesis of white or brown fats are also discussed. The function of lncRNAs as decoy molecules and RNA double-stranded complexes, among other functions, is also discussed.

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lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene

Cited by 9 publications

References 41 publications

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

LincRNA-MSTRG.673.2 Promotes Chicken Intramuscular Adipocyte Differentiation by Sponging miR- 128-3p

The role of the novel lncRNA lncFABP4 in modulating adipogenic differentiation in buffalo intramuscular preadipocytes

LncRNA-Mediated Adipogenesis in Different Adipocytes

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