LncRNA-Mediated Adipogenesis in Different Adipocytes

Zhang, Peiwen; Wu, Shuang; He, Yuxu; Li, Xinrong; Zhu, Yan; Lin, Xutao; Chen, Lei; Zhao, Ye; Niu, Lili; Zhang, Shunhua; Li, Xuewei; Zhu, Li; Shen, Linyuan

doi:10.3390/ijms23137488

Cited by 31 publications

(13 citation statements)

References 154 publications

(158 reference statements)

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“…It is critical to study the biological function of adipocytes in food-producing animals. Recently, lncRNAs have emerged as crucial regulators that regulate lipid accumulation or white fat browning in adipocytes in multiple ways. , Using RNA-seq, Yang et al found 501 DE-lncRNAs at different differentiation stages (0, 3, 6, and 9 d) of bovine intramuscular preadipocytes . Li et al identified 16 differentially expressed lncRNAs related to the late stage of adipocyte differentiation .…”

Section: Discussionmentioning

confidence: 99%

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LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis

Li,

Hou,

Meng

et al. 2024

J. Agric. Food Chem.

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Research on adipogenesis will help to improve the meat quality of livestock. Long noncoding RNAs (lncRNAs) are involved in mammalian adipogenesis as epigenetic modulators. In this study, we analyzed lncRNA expression during bovine adipogenesis and detected 195 differentially expressed lncRNAs, including lncRNA BlncAD1, which was significantly upregulated in mature bovine adipocytes. Gain-and loss-of-function experiments confirmed that BlncAD1 promoted the proliferation, apoptosis, and differentiation of bovine preadipocytes. RNA pull-down revealed that the nonmuscle myosin 10 (MYH10) is a potential binding protein of BlncAD1. Then, we elucidated that loss of BlncAD1 caused increased ubiquitination of MYH10, which confirmed that BlncAD1 regulates adipogenesis by enhancing the stability of the MYH10 protein. Western blotting was used to demonstrate that BlncAD1 activated the PI3K/Akt signaling pathway. Bioinformatic analysis and dual-luciferase reporter assays indicated that BlncAD1 competitively absorbed miR-27a-5p. The overexpression and interference of miR-27a-5p in bovine preadipocytes displayed that miR-27a-5p inhibited proliferation, apoptosis, and differentiation. Further results suggested that miR-27a-5p targeted the CDK6 gene and that BlncAD1 controlled the proliferation of bovine preadipocytes by modulating the miR-27a-5p/CDK6 axis. This study revealed the complex mechanisms of BlncAD1 underlying bovine adipogenesis for the first time, which would provide useful information for genetics and breeding improvement of Chinese beef cattle.

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Section: Discussionmentioning

confidence: 99%

“…adipocytes in food-producing animals. Recently, lncRNAs have emerged as crucial regulators that regulate lipid accumulation or white fat browning in adipocytes in multiple ways 29,30. Using RNA-seq, Yang et al found 501 DE-lncRNAs at different differentiation stages (0, 3, 6, and 9 d) of bovine intramuscular preadipocytes 31.…”

mentioning

confidence: 99%

LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis

Li,

Hou,

Meng

et al. 2024

J. Agric. Food Chem.

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“…Many lncRNAs associated with adipogenesis have recently been reported in animals, playing a crucial role in animal fat deposition. 32,33 In plants, lncRNA has been reported to be involved in oil synthesis in B. napus, O. sativa, Paeonia suffruticosa, and J. curcas. Here, the lncRNA43234 gene was screened from the lncRNA−miRNA−mRNA coexpression network based on pretranscriptome sequencing.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Many lncRNAs associated with adipogenesis have recently been reported in animals, playing a crucial role in animal fat deposition. , In plants, lncRNA has been reported to be involved in oil synthesis in B. napus, O.…”

Section: Discussionmentioning

confidence: 99%

Analysis of LncRNA43234-Associated ceRNA Network Reveals Oil Metabolism in Soybean

Zhang

Wang

et al. 2023

J. Agric. Food Chem.

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Soybean [Glycine max (Linn.) Merr.] is an important oil crop. Long noncoding RNAs (lncRNAs) play a variety of functions in plants. However, their function in the soybean oil synthesis pathway is yet to be uncovered. Here, the lncRNA43234 gene related to soybean oil synthesis was screened, and the full-length cDNA sequence of the lncRNA was obtained using rapid amplification of cDNA ends. Overexpression of lncRNA43234 increased the content of crude protein in seeds, decreased the content of oleic acid, and affected the content of alanine and arginine in free amino acids. RNA interference of the lncRNA43234 gene decreased the crude protein content in seeds. Quantitative real-time polymerase chain reaction analysis revealed that lncRNA43234 influenced the expression of XM_014775786.1 associated with phosphatidylinositol metabolism by acting as a decoy for miRNA10420, thereby affecting the content of soybean oil. Our results provide insights into how lncRNA-mediated competing endogenous RNA regulatory networks are involved in soybean oil synthesis.

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“…Recent findings have revealed that lncRNAs can interact with protein‐coding genes and regulate them by directly competing with the binding of microRNAs and therefore participate as competing endogenous RNAs in the regulation of fundamental cellular processes (Xu et al., 2022; Zhao et al., 2023). Moreover, a plethora of evidence suggests the contribution of lncRNAs in the regulation of metabolism and fat deposition (Lu et al., 2021; Zhang et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

LncRNAENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR‐146b/AGPAT2 pathway

Xing,

2024

Animal Genetics

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The liver contributes to lipid metabolism as the hub of fat synthesis. Long non‐coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes’ lipid metabolism. Thirty‐two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR‐146b along with the levels of proteins involved in the regulation of fat metabolism, lipid indices and oxidative stress were measured. Moreover, primary chicken hepatocytes were transfected with lncRNA 21 686 small interfering RNA or microRNA (miRNA, miR)‐146b mimics to measure the consequences of suppressing lncRNA or inducing miRNA expression on the levels of proteins involved in fat metabolism and stress markers. The results showed that the high‐fat diet modulated the expression of lncRNA 21 686 and miR‐146b (p‐value < 0.001). Moreover, there was a significant increase in 1‐acyl‐sn‐glycerol‐3‐phosphate acyltransferase 2 (AGPAT2) gene expression and protein levels and modulated fat‐related markers. Furthermore, the results showed that lncRNA 21 686 suppression reduced the expression of AGPAT2 and its downstream proteins (p‐value < 0.05). Overexpression of miR‐146b regulated fat metabolism indicator expression. Transfection experiments revealed that lncRNA 21 686 suppression increased miR‐146b expression. The findings suggested a novel mechanism containing lncRNA 21 686/miR‐146b/AGPAT2 in the regulation of fat metabolism in chicken hepatocytes.

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LncRNA-Mediated Adipogenesis in Different Adipocytes

Cited by 31 publications

References 154 publications

LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis

LncRNA BlncAD1 Modulates Bovine Adipogenesis by Binding to MYH10, PI3K/Akt Signaling Pathway, and miR-27a-5p/CDK6 Axis

Analysis of LncRNA43234-Associated ceRNA Network Reveals Oil Metabolism in Soybean

LncRNAENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR‐146b/AGPAT2 pathway

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