LncRNAs expression profiling in normal ovary, benign ovarian cyst and malignant epithelial ovarian cancer

Abstract

Long noncoding RNA (lncRNA) has been recognized as a regulator of gene expression, and the dysregulation of lncRNAs is involved in the progression of many types of cancer, including epithelial ovarian cancer (EOC). To explore the potential roles of lncRNAs in EOC, we performed lncRNA and mRNA microarray profiling in malignant EOC, benign ovarian cyst and healthy control tissues. In this study, 663 transcripts of lncRNAs were found to be differentially expressed in malignant EOC compared with benign and normal control tissues. We also selected 18 altered lncRNAs to confirm the validity of the microarray analysis using quantitative real-time PCR (qPCR). Pathway and Gene Ontology (GO) analyses demonstrated that these altered transcripts were involved in multiple biological processes, especially the cell cycle. Furthermore, Series Test of Cluster (STC) and lncRNA-mRNA co-expression network analyses were conducted to predict lncRNA expression trends and the potential target genes of lncRNAs. We also determined that two antisense lncRNAs (RP11-597D13.9 and ADAMTS9-AS1) were associated with their nearby coding genes (FAM198B, ADAMTS9), which participated in cancer progression. This study offers helpful information to understand the initiation and development mechanisms of EOC.

Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer

“…Recent studies have shown that long noncoding RNAs (lncRNAs), a class of noncoding RNA transcripts of 200 nucleotides in length, can distinguish markedly between ovarian carcinoma tissue and corresponding normal tissue. 3 LncRNAs frequently function as tumor suppressors or oncogenes. For example, a low level of lncRNA GAS5 produced a poorer overall survival rate against ovarian cancer.…”

Aberrant expression of imprinted lncRNA MEG8 causes trophoblast dysfunction and abortion

“…Abnormal expression of lncRNAs is involved in the pathogenesis of various diseases. In recent years, many studies have used microarray technology to screen for differentially expressed lncRNAs in the occurrence of disease . Exploring the mechanism of disease regulation or new mechanisms have become common practice.…”

Long non‐coding RNA RP11‐552M11.4 promotes cells proliferation, migration and invasion by targeting BRCA2 in ovarian cancer

“…A previous study observed 663 dysregulated lncRNAs in EOC patients compared to benign or normal controls determined by Arraystar Human lncRNA Microarray V3.0 . Another recent study detected lncRNAs expression profiles in 5 paired human EOC tissues and adjacent non‐tumor tissue samples by Arraystar Human LncRNA Microarray V3.0, and discovered 672 up‐regulated and 549 down‐regulated lncRNAs in EOC tissue .…”