LncRNA ST8SIA6-AS1 promotes hepatocellular carcinoma progression by regulating MAGEA3 and DCAF4L2 expression

Zhang, Xiufen; Xu, Sui; Hu, Chen; Fang, Kai; Zhou, Junjing; Guo, Zijian; Zhu, Guoding; Li, Lihua

doi:10.1016/j.bbrc.2020.09.115

Cited by 18 publications

(12 citation statements)

References 27 publications

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“…First, 293T cells (ATCC) were seeded onto 6-well plates at a density of 2x10 5 cells per well. After 12 h, the lentivirus was packaged into the 293T cells following common protocols (17), at a multiplicity of infection of 10. Viral particles were harvested at 48 h after transfection.…”

Section: Methodsmentioning

confidence: 99%

lncRNA ST8SIA6‑AS1 facilitates proliferation and invasion in liver cancer by regulating miR‑142‑3p

et al. 2021

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Long non-coding RNA ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase 6 antisense 1 (ST8SIA6-AS1) has been identified as a novel oncogene in breast cancer. However, its involvement in liver cancer has remained elusive. In the present study, the expression of ST8SIA6-AS1 and microRNA (miR)-142-3p in liver cancer tissues and cell lines was detected by reverse transcription-quantitative PCR. Tumor cell proliferation, migration and invasion assays were performed to determine the biological functions of ST8SIA6-AS1. The targeting interaction between ST8SIA6-AS1 and miR-142-3p predicted by bioinformatics was verified by a luciferase reporter assay and a biotin pulldown assay. The results indicated that ST8SIA6-AS1 was highly expressed in liver cancer tissues and cell lines, and the high expression of ST8SIA6-AS1 in liver cancer tissues was associated with poor prognosis. Knockdown of ST8SIA6-AS1 inhibited the proliferation, metastasis and invasion of liver cancer cells. Mechanistic investigation revealed that ST8SIA6-AS1 sequesters miR-142-3p and negatively regulates miR-142-3p expression in liver cancer cells. Further investigation indicated that the tumor-inhibitory effect of ST8SIA6-AS1 silencing was reversed by miR-142-3p depletion. In conclusion, ST8SIA6-AS1 was indicated to exert an oncogenic function in liver cancer by competitively sponging miR-142-3p.

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Section: Methodsmentioning

confidence: 99%

lncRNA ST8SIA6‑AS1 facilitates proliferation and invasion in liver cancer by regulating miR‑142‑3p

et al. 2021

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“…ST8Sia6 generates disialic acid structures, eventually by transfer of a sialic acid moiety onto a NeuAca2,3 (6)Gal disaccharide on acceptor substrates, which include glycolipids, but preferentially O-linked glycoproteins (234). Some investigators suggest that ST8SIA6 Antisense RNA 1 (ST8SIA6-AS1) is associated with poor prognosis and enhances the proliferative and metastatic potential of cancer cells (235)(236)(237)(238)(239)(240). Furthermore, ST8Sia6 may increase the chemosensitivity of tumor cells at least to certain drugs (226).…”

Section: St8sia6mentioning

confidence: 99%

The Distinct Roles of Sialyltransferases in Cancer Biology and Onco-Immunology

et al. 2021

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Aberrant glycosylation is a key feature of malignant transformation. Hypersialylation, the enhanced expression of sialic acid-terminated glycoconjugates on the cell surface, has been linked to immune evasion and metastatic spread, eventually by interaction with sialoglycan-binding lectins, including Siglecs and selectins. The biosynthesis of tumor-associated sialoglycans involves sialyltransferases, which are differentially expressed in cancer cells. In this review article, we provide an overview of the twenty human sialyltransferases and their roles in cancer biology and immunity. A better understanding of the individual contribution of select sialyltransferases to the tumor sialome may lead to more personalized strategies for the treatment of cancer.

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“…Indeed, ST8SIA6-AS1 is upregulated in HCC and can facilitate cell proliferation and resistance to cell apoptosis via sponging miR-4656 and upregulation of HDAC11 expression, suggesting an important role of the ST8SIA6-AS1/miR-4656/HDAC11 axis in regulating HCC cell proliferation [45]. Consistently, downregulation of ST8SIA6-AS1 suppressed cell proliferation in vitro and restrained HCC tumorigenesis in vivo [46]. A similar role is played by lncRNA ST8SIA6-AS1 in breast cancer through the p38 MAPK signaling pathway [47].…”

Section: Sustaining Proliferation and Tumor Growthmentioning

confidence: 88%

“…Recently, the lncRNA ST8SIA6-AS1 has been shown to promote migration and invasion in breast cancer cells through the p38 MAPK signaling [ 47 ], in HCC cells by regulating the expression of the Cul4-associated factor 4-like 2 (DCAF4L2) [ 46 ] and in lung adenocarcinoma cells by sponging miR-125a-3p to increase nicotinamide N-methyltransferase (NNMT) expression [ 36 ] ( Table 2 ).…”

Section: Roles Of Sialyltransferases In Cancermentioning

confidence: 99%

Aberrant Sialylation in Cancer: Biomarker and Potential Target for Therapeutic Intervention?

Pietrobono¹,

Stecca²

2021

Cancers

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Sialylation is an integral part of cellular function, governing many biological processes including cellular recognition, adhesion, molecular trafficking, signal transduction and endocytosis. Sialylation is controlled by the levels and the activities of sialyltransferases on glycoproteins and lipids. Altered gene expression of these enzymes in cancer yields to cancer-specific alterations of glycoprotein sialylation. Mounting evidence indicate that hypersialylation is closely associated with cancer progression and metastatic spread, and can be of prognostic significance in human cancer. Aberrant sialylation is not only a result of cancer, but also a driver of malignant phenotype, directly impacting key processes such as tumor cell dissociation and invasion, cell-cell and cell-matrix interactions, angiogenesis, resistance to apoptosis, and evasion of immune destruction. In this review we provide insights on the impact of sialylation in tumor progression, and outline the possible application of sialyltransferases as cancer biomarkers. We also summarize the most promising findings on the development of sialyltransferase inhibitors as potential anti-cancer treatments.

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LncRNA ST8SIA6-AS1 promotes hepatocellular carcinoma progression by regulating MAGEA3 and DCAF4L2 expression

Cited by 18 publications

References 27 publications

lncRNA ST8SIA6‑AS1 facilitates proliferation and invasion in liver cancer by regulating miR‑142‑3p

lncRNA ST8SIA6‑AS1 facilitates proliferation and invasion in liver cancer by regulating miR‑142‑3p

The Distinct Roles of Sialyltransferases in Cancer Biology and Onco-Immunology

Aberrant Sialylation in Cancer: Biomarker and Potential Target for Therapeutic Intervention?

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