LncRNA PSMB8-AS1 acts as ceRNA of miR-22-3p to regulate DDIT4 expression in glioblastoma

Characterization of Long Non-Coding RNAs in Systemic Sclerosis Monocytes: A Potential Role for PSMB8-AS1 in Altered Cytokine Secretion

“…In line with previous observations of three other studies in pancreatic epithelial cell lines and glioma [ 37 , 38 , 39 ], our subcellular localization analysis demonstrated that PSMB8-AS1 is present in the cytoplasm of CD14+ monocytes. Given that cytoplasmic lncRNAs can regulate secretory and extracellular vesicles [ 47 ], and PSMB8-AS1 was present in co-expression modules annotated to vesicle related transport, it is possible that this lncRNA is involved in the regulation of cytoplasmic vesicles.…”

“…The percentage of enrichment of IL-8 Primary Transcript and IL-8 mRNA were used as positive controls to verify the appropriate separation of different compartments, as they are expected to be found in the nucleus/chromatin or cytoplasm, respectively. In agreement with previous findings in epithelial, glioma, and pancreatic cells [37][38][39], PSMB8-AS1 was found to be enriched in monocyte cytoplasm (Figure 4F, third panel), suggesting that this lncRNA may regulate expression of its target(s) at the post-transcriptional level [29].…”

“…Both the cis correlation analysis and co-expression network analysis pointed at the lncRNA PSMB8-AS1 as a key regulator of altered gene-expression in SSc monocytes. Although this lncRNA has previously been described in the context of influenza infection [ 44 ] and cancer [ 37 , 38 , 39 ], our results link PSMB8-AS1 dysregulation with autoimmunity for the first time. The cis correlation analysis predicted PSMB8, the protein coding gene located antisense to PSMB8-AS1, as a potential target of this lncRNA; however, the two genes were not present in the same co-expression modules, and PSMB8 mRNA levels were not altered upon PSMB8-AS1 silencing.…”

“…To further investigate the biological relevance of PSMB8-AS1, its expression was efficiently silenced in resting and R848 stimulated monocytes using a specific small interfering RNA (siRNA) ( Figure 5 A, p < 0.001). The impact of PSMB8-AS1 silencing on monocyte apoptosis was first investigated, as this lncRNA has previously been reported as a negative regulator of apoptotic signaling [ 37 , 38 , 39 ], and apoptotic signaling pathways were also identified by our GO-term enrichment analysis in the cis correlation analysis. However, FACS analysis demonstrated that PSMB8-AS1 silencing in monocytes did not affect apoptosis, neither in resting nor stimulated conditions ( p -value > 0.05, Figure S5A,B ).…”

<p>Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p</p>

“…28 Increasing investigations reveal a ceRNA mechanism regulated by lncRNAs in diverse malignancies. 29 In the current study, cytoplasm and nuclear fractions test uncovered that linc01094 was primarily localized in cytoplasm, so we hypothesized that linc01094 might function as a ceRNA. In addition, RNA-FISH and RIP assays demonstrated the direct interaction between linc01094 and miR-126-5p.…”