LncRNA profile in Hashimoto’s thyroiditis and potential function of NONHSAT079547.2

Luo, Jingyi; T, Liu; Wang, Teng

doi:10.1530/jme-19-0239

Cited by 4 publications

(4 citation statements)

References 35 publications

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“…In addition, many lncRNAs also play a significant role in the regulation of immunity, such as lncRNA UCA1 attenuated the killing effect of cytotoxic CD8 + T cells on anaplastic thyroid carcinoma (ATC) cells through the miR-148a/PD-L1 pathway (28). Afterwards, further studies confirmed that lncRNA NONHSAT079547.2 could promote cell growth and control IL-17 expression and secretion via the NONHSAT079547.2/miR-4716-5p/IL-17 axis in CD4+ T cells of HT patients (29). However, these lncRNAs were not found in our lncRNA microarrays, which may be due to different regulatory mechanisms bewteen PTC/HT(+) and PTC/HT(-).…”

Section: Discussionmentioning

confidence: 94%

Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto’s Thyroiditis

Ding

Peng

et al. 2022

Front. Oncol.

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Long noncoding RNAs (lncRNAs) play a significant role in cancer biology. This study aimed to determine the roles of lncRNAs in establishing the differences in clinical features between patients with papillary thyroid carcinoma (PTC) without Hashimoto’s thyroiditis (HT) and patients with PTC and HT. In the present study, we detected the differentially expressed lncRNAs between tumor tissues of patients with PTC with or without HT through lncRNA microarrays. The data were verified and analyzed through qRT-PCR, cell viability, cell cycle and bioinformatics analyses. We found that 1031 lncRNAs and 1338 mRNAs were abnormally expressed in 5 tissue samples of PTC complicated with HT [PTC/HT (+)] compared with 5 samples of PTC without HT [PTC/HT (-)]. Gene Ontology and pathway analyses of the mRNAs suggested that several biological processes and pathways, particularly immune system processes, were induced in the PTC/HT (+) tissues. Twenty lncRNAs were verified in 31 PTC/HT (+) and 64 PTC/HT (-) specimens by qRT-PCR, and the results were consistent with the microarray data. Specifically, ENST00000452578, a downregulated lncRNA in PTC/HT(+), was negatively correlated with the tumor size. Cell viability assays revealed that ENST00000452578 could inhibit cell proliferation. Our results indicate that lncRNAs and mRNAs play an important role in establishing the different clinical characteristics between patients with PTC/HT(+) and patients with PTC/HT(-), and might provide new insights from the perspective of RNA for obtaining a further understanding of the clinical features related to PTC with HT.

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Section: Discussionmentioning

confidence: 94%

Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto’s Thyroiditis

Ding

Peng

et al. 2022

Front. Oncol.

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“…Maternally expressed 3 has been identified as a competing endogenous RNA of miR-17 that regulates the level and function of Th17 cells in asthma ( 45 ). It has also been shown that NONHSAT079547.2 could promote cell proliferation, as well as control IL-17 expression and secretion via the miR-4716-5p/IL-17 axis in CD4 + T cells ( 25 ). lnc-DC regulates dendritic cells differentiation by directly binging to STAT3 and promoting STAT3 phosphorylation ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

“…In total, 32 age- and sex-matched healthy subjects were included as controls, including five healthy female volunteers for NGS and 27 healthy volunteers for validation of the sequencing results. All healthy subjects were euthyroid and free of thyroid-specific autoantibodies, as well as had no history of thyroid disease, chronic inflammatory diseases, tumors and other autoimmune diseases ( 25 ). All subjects showed no obvious clinical symptoms of infection and were not taking immunosuppressive drugs.…”

Section: Methodsmentioning

confidence: 99%

Long non‑coding RNA expression profiles identify lncRNA‑XLOC_I2_006631 as a potential novel blood biomarker for Hashimoto's thyroiditis

et al. 2020

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Long non-coding RNAs (lncRNAs) have been increasingly recognized as important immune checkpoints involved in the pathogenesis of autoimmune diseases. However, the exact role of lncRNAs in Hashimoto's thyroiditis (HT) has been rarely studied. The aim of the present study was to investigate the role of lncRNAs and the potential biomarkers in HT, a total of 33 patients with HT and 32 healthy volunteers were enrolled in the present study, and five patients and five healthy controls were investigated using next generation sequencing. A total of 218 dysregulated lncRNAs, including 94 upregulated and 124 downregulated lncRNAs, were identified and examined in the peripheral blood mononuclear cells (PBMCs) from patients with HT. The majority of the lncRNAs were intergenic and exonic (66.06%). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that abnormally expressed lncRNAs were enriched in the 'NF-kB expression', in the 'TGF-β signaling pathway' and in the 'JAK-STAT signaling pathway', which are associated with the immunopathogenic mechanisms of HT. In total, three lncRNAs (LOC729737, XLOC_I2_006631 and BC041964) were validated and had a trend identical to that detected by the sequencing results. The expression of lncRNA-XLOC_I2_006631 was upregulated and was positively correlated with the serum concentrations of anti-thyroperoxidase antibody in patients with HT. Methyl-CpG-binding protein 2 (MECP2) was identified as the potential regulatory gene of lncRNA-XLOC_I2_006631 using a prediction program. The expression of MECP2 was increased and was positively correlated with the elevated expression levels of lncRNA-XLOC_I2_006631 and anti-thyroperoxidase antibody in patients with HT. Furthermore, lncRNA-XLOC_I2_006631 was able to regulate MECP2 expression in vitro . Receiver operating characteristic curve analysis suggested that lncRNA-XLOC_I2_006631 has a potential diagnostic value. Collectively, the present results indicated the important role of dysregulated lncRNAs in HT and demonstrated that lncRNA-XLOC_I2_006631 functioned as a positive regulator of MECP2 expression, suggesting a potential mechanism. Thus, lncRNA-XLOC_I2_006631 may be used as a biomarker of HT.

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“…The peripheral Th cell-expressed lncRNA NONHSAT079547.2 was positively correlated with IL−17 expression in Hashimoto’s thyroiditis. Further investigation suggested a mechanism by which NONHSAT079547.2 acts as a ceRNA to IL17 mRNA, where it competitively binds with miR−4716-5p, which could directly target the IL17 mRNA sequence [ 74 ].…”

Section: Lncrnas and Psoriasismentioning

confidence: 99%

Implications of LncRNAs and CircRNAs in psoriasis: a review

Shi

Jiang

et al. 2023

RNA Biology

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Psoriasis is a chronic inflammatory skin disease characterized by skin infiltration of immune cells and abnormal epidermal thickening. The initial pathogenesis has not been fully elucidated. Non-coding RNAs (ncRNAs), which include long ncRNAs (lncRNAs) and circular RNAs (circRNAs), comprise the majority of genome transcripts and are important influencers of gene transcription and post-transcription modulations. Emerging roles of ncRNAs in psoriasis were identified recently. This review summarizes the existing studies of psoriasis-related lncRNAs and circRNAs. A considerable proportion of the studied lncRNAs and circRNAs regulate keratinocyte mobility, such as keratinocyte proliferation and differentiation. Some lncRNAs and circRNAs are tightly related to keratinocyte inflammation reactions. Other reports demonstrated that they are also implicated in modulating immune cell differentiation, proliferation, and activation. This review might illuminate future psoriasis research and highlight that lncRNAs and circRNAs might act as therapeutic targets.

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LncRNA profile in Hashimoto’s thyroiditis and potential function of NONHSAT079547.2

Cited by 4 publications

References 35 publications

Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto’s Thyroiditis

Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto’s Thyroiditis

Long non‑coding RNA expression profiles identify lncRNA‑XLOC_I2_006631 as a potential novel blood biomarker for Hashimoto's thyroiditis

Implications of LncRNAs and CircRNAs in psoriasis: a review

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