LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells

Zou, Yanmei; Yao, Shuo; Chen, Xiuqiong; Liu, Dian; Wang, Jianhua; Yuan, Xun; Rao, J. V.; Xiong, Huihua; Yu, Shiying; Yuan, Xianglin; Zhu, Feng; Hu, Guohong; Wang, Yihua; Xiong, Hua

doi:10.1016/j.ejcb.2018.04.005

Cited by 99 publications

(76 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the determination of their critical roles in cancer development is also dependent on their location in cells, which in other words, access to interaction with different molecules distributed either in the nucleus or cytoplasm provides a flexible mechanism of actions of lncRNAs. On the one hand, lncRNA OIP5-AS1 involved in this study, a tumor promoter in several cancers, [19][20][21][22][23] was not documented to partake in GC oncogenesis. 30 Regulatory ncRNAs such as PCAT6, 31 LINC00968, 32 TUG1, 33 and CASC15 34 were reported to interact with EZH2, increasing F I G U R E 3 OIP5-AS1 represses NLRP6 expression by recruiting EZH2, increasing the H3K27me3 enrichment of NLRP6 promoter.…”

Section: Discussionmentioning

confidence: 64%

“…EZH2, enhancer of zeste homolog 2; mRNA, messenger RNA; NLRP6, Nod-like receptor pyrin domain-containing protein 6; OIP5-AS1, OIP5 antisense RNA 1; qRT-PCR, real-time quantitative polymerase chain reaction trimethylation of H3K27 on the target gene promoters. On the one hand, lncRNA OIP5-AS1 involved in this study, a tumor promoter in several cancers, [19][20][21][22][23] was not documented to partake in GC oncogenesis. On the other hand, the mechanism of OIP5-AS1-recruited EZH2 regulates tumor proceedings has never been noted either.…”

Section: Discussionmentioning

confidence: 64%

“…14,15 And lncRNA-mediated epigenetic inhibition of antitumor gene via binding EZH2 in various cancers has been gradually detected. 18 Recently, a newly identified lncRNA OIP5-AS1 has been characterized to promote the tumorigenesis of osteosarcoma, 19 cervical cancer, 20 lung cancer, 21 colorectal cancer, 22 and bladder cancer. 16 TUG1 confers adriamycin resistance in acute myeloid leukemia by epigenetically suppressing miR-34a expression via EZH2.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Long noncoding RNA OIP5‐AS1 aggravates cell proliferation, migration in gastric cancer by epigenetically silencing NLRP6 expression via binding EZH2

Bai

Li²

2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

The critical role of long noncoding RNAs (lncRNAs) in the development of multiple cancers has been revealed either functioning as a tumor initiator or a cancer suppressor. A widely recognized OIP5 antisense RNA 1 (lncRNA OIP5‐AS1) has been validated to be an essential regulator of the tumorigenesis of various malignancies. Whereas, the potential role and the exact mechanism of lncRNA OIP5‐AS1 by which OIP5‐AS1 mediates gastric cancer (GC) progression remains vague. Therefore, first our work probed its expression levels in GC cell lines and related normal cells by real‐time quantitative polymerase chain reaction. The heightened level of OIP5‐AS1 was detected in GC cell lines. In terms of its cellular effects, we performed a series of functional experiments and as presented in the assays, the proliferative potential and motility was diminished. However, more apoptotic cells were induced with the introduction of OIP5‐AS1 silencing. Meanwhile, higher Nod‐like receptor pyrin domain‐containing protein 6 (NLRP6) and enhancer of zeste homolog 2 (EZH2) expression in the GC cells was monitored. Besides, OIP5‐AS1 was disclosed to locate mainly in the nucleus. In terms of mechanism, OIP5‐AS1 directly bound to EZH2 and obstructed NLRP6 expression, speeding up GC progression.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Long noncoding RNA OIP5‐AS1 aggravates cell proliferation, migration in gastric cancer by epigenetically silencing NLRP6 expression via binding EZH2

Bai

Li²

2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Previous studies showed that lncRNA OIP5‐AS1 acted as a motivator in various human tumors, such as glioma, colorectal cancer, hepatoblastoma and lung cancer . However, the expression and function roles of lncRNA OIP5‐AS1 were still unclear in CC progression.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA opa‐interacting protein 5 antisense transcript 1 promotes proliferation and invasion through elevating integrin α6 expression by sponging miR‐143‐3p in cervical cancer

Yang

Jiang

Hai

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

An increasing number of studies have shown that long noncoding RNAs (lncRNAs) play important roles in cervical cancer (CC) progression. However, the roles and underlying mechanisms of lncRNA opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) involved in the CC remain unclear. In the current study, we found that lncRNA OIP5-AS1 was upregulated in CC tissues and cell lines. High OIP5-AS1 expression was significantly correlated with advanced International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and poor overall survival of patients with CC. Using in vitro function assays, we showed that OIP5-AS1 suppression significantly decreased the proliferation, colony formation, and invasion ability of CC cells. Moreover, we revealed that OIP5-AS1 could act as a competing endogenous RNA of miR-143-3p to regulate the ITGA6 expression. Rescue assays showed that miR-143-3p inhibitors or ITGA6 overexpression could reverse the inhibitory effects of OIP5-AS1 suppression on the proliferation and invasion in CC cells. In addition, OIP5-AS1 suppression reduced tumor growth in vivo. In conclusion, we demonstrated that OIP5-AS1 promoted proliferation and invasion of CC cells via increasing the ITGA6 expression by sponging miR-143-3p, which might be an effective therapeutic target for the treatment of patients with CC.

show abstract

“…For instance, Zheng et al (2016) showed that small nucleolar RNA host gene 18 (SNHG18) was highly expressed in glioma tissues compared that in paired normal tissues, and knockdown of SNHG18 enhanced the radiosensitivity of glioma cells through repressing semaphorin 5A (Zheng et al, 2016). Zou et al (2018) stated that lncRNA OIP5-AS1 was downregulated in radioresistant colorectal cancer cells and knockdown of OIP5-AS1 increased cell viability, reduced radio-induced cell apoptosis, and reinforced the radiosensitivity of LoVo cells through regulating microRNA-369-3p (miR-369-3p)/DYRK1A axis. miRNAs, small non-coding, single-stranded RNA with 20-22 nucleotides in length, serve as pivotal modulators in several biological processes through modulating gene expression at transcriptional and post-transcriptional levels (Sun et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis

Chen

Zhang

2018

Cell Biology International

View full text Add to dashboard Cite

Radioresistance is a major obstacle in hepatocellular carcinoma (HCC) radiotherapy. Aberrant expression of long non-coding RNA (lncRNA) has been postulated to be implicated in the development of HCC radioresistance. We investigated the role of lncRNA nuclear enriched abundant transcript 1_2 (NEAT1_2) in radioresistance of HCC and its molecular mechanism in this study. We found that NEAT1_2 and WEE1 were upregulated, and miR-101-3p was downregulated in HCC tissues, as well as HCC cell lines. Downregulation of WEE1 sensitized the radiosensitivity of HCC cells, as evidenced by decreased survival fractions of Huh7 and PLC5 cells and increased percentage of apoptotic cells. Also, knockdown of NEAT1_2 exerted a reinforcing effect on the radiosensitivity of HCC cells. In addition, WEE1 was confirmed as a direct target of miR-101-3p. Upregulation of miR-101-3p obviously decreased the mRNA and protein levels of WEE1 compared with that in the miR-NC group, while transfection of anta-miR-101-3p presented the opposite effects. In parallel, NEAT1_2 was identified to interact with miR-101-3p, and NEAT1_2 upregulated the expression of WEE1 in Huh7 cells through sponging miR-101-3p. Besides, the reinforcing effect of NEAT1_2 silencing could be attenuated by downregulation of miR-101-3p. To conclude, our results support the concept that downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR-101-3p/WEE1 axis.

show abstract

LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells

Abstract: LncRNA OIP5-AS1 suppressed cell viability, promoted radio-induced apoptosis, and enhanced the radiosensitivity of CRC cells by regulating DYRK1A expression through miR-369-3p.

Cited by 99 publications

References 30 publications

Long noncoding RNA OIP5‐AS1 aggravates cell proliferation, migration in gastric cancer by epigenetically silencing NLRP6 expression via binding EZH2

Long noncoding RNA OIP5‐AS1 aggravates cell proliferation, migration in gastric cancer by epigenetically silencing NLRP6 expression via binding EZH2

Long noncoding RNA opa‐interacting protein 5 antisense transcript 1 promotes proliferation and invasion through elevating integrin α6 expression by sponging miR‐143‐3p in cervical cancer

Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis

Contact Info

Product

Resources

About