LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

Tian, Yuanye; Shao, Qi; Gu, Jiahong; Tang, Yi; Bie, Miaomiao; Zhou, Yangyifan; Cheng, Chunan; Liang, Yi; Zhang, Qian; Kang, Feiwu

doi:10.3389/fgene.2022.909095

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…Actually, lncRNA profiles are altered during osteoclast differentiation. The altered potential of differentiation also influences lncRNA profiles [11] . lncRNAs are involved in modulating osteoclast differentiation.…”

Section: Discussionmentioning

confidence: 99%

lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis

Zhang,

Yao

et al. 2023

ABBS

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Long noncoding RNAs (lncRNAs) are important regulators of bone metabolism. In this study, lncRNA microarray analysis was used to identify differentially expressed lncRNAs in differentiated osteoclasts. lncRNA-Gm5532 is highly expressed during osteoclast differentiation. lncRNA-Gm5532 knockdown impairs osteoclast formation and bone resorption. Mechanistic experiments show that lncRNA-Gm5532 functions as a competing endogenous RNA (ceRNA) and acts as a sponge for miR-125a-3p, which promotes TNF receptor-associated factor 6 (TRAF6) expression. miR-125a-3p mimics suppress osteoclast differentiation and TAK1/NF-κB/MAPK signaling. The miR-125a-3p inhibitor reverses the negative effects of siGm5532 on osteoclast differentiation. In summary, our study reveals that lncRNA-Gm5532 functions as an activator in osteoclast differentiation by targeting the miR-125a-3p/TRAF6 axis, making it a novel biomarker and potential therapeutic target for osteoporosis.

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Section: Discussionmentioning

confidence: 99%

lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis

Zhang,

Yao

et al. 2023

ABBS

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“…LncRNA participates in bone development and regeneration by regulating apoptosis, angiogenesis, and BMSCs differentiation [ 171 ]. The gene sequencing results showed that 95 lncRNAs were differentially expressed in osteoclasts knocked out of HIF-1α, suggesting that lncRNA may play a role in bone homeostasis regulation by HIF-1α [ 172 ]. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), as a highly conserved lncRNA, induces angiogenesis through the mTOR/HIF-1α pathway [ 173 ].…”

Section: The Role Of Non-coding Rna (Ncrna) In Hif-1α Regulating Bone...mentioning

confidence: 99%

HIF-1α Regulates Bone Homeostasis and Angiogenesis, Participating in the Occurrence of Bone Metabolic Diseases

Tang

et al. 2022

Cells

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In the physiological condition, the skeletal system’s bone resorption and formation are in dynamic balance, called bone homeostasis. However, bone homeostasis is destroyed under pathological conditions, leading to the occurrence of bone metabolism diseases. The expression of hypoxia-inducible factor-1α (HIF-1α) is regulated by oxygen concentration. It affects energy metabolism, which plays a vital role in preventing bone metabolic diseases. This review focuses on the HIF-1α pathway and describes in detail the possible mechanism of its involvement in the regulation of bone homeostasis and angiogenesis, as well as the current experimental studies on the use of HIF-1α in the prevention of bone metabolic diseases. HIF-1α/RANKL/Notch1 pathway bidirectionally regulates the differentiation of macrophages into osteoclasts under different conditions. In addition, HIF-1α is also regulated by many factors, including hypoxia, cofactor activity, non-coding RNA, trace elements, etc. As a pivotal pathway for coupling angiogenesis and osteogenesis, HIF-1α has been widely studied in bone metabolic diseases such as bone defect, osteoporosis, osteonecrosis of the femoral head, fracture, and nonunion. The wide application of biomaterials in bone metabolism also provides a reasonable basis for the experimental study of HIF-1α in preventing bone metabolic diseases.

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“…Hypoxia-inducible factor 1α (HIF-1α) has been implicated in the pathogenesis of RA and it participates in osteoclast differentiation by regulating nutrient and energy sensors ( Fearon et al, 2016 ; Tang et al, 2019 ). Therefore, another study explored the lncRNA-mRNA expression profiles associated with HIF-1α-knockout mouse osteoclast differentiation by RNA-seq ( Tian et al, 2022 ). The results showed that lncRNA MSTRG.31769.2 and MSTRG.7566.12 were the two lncRNAs with the two highest numbers of interactions.…”

Section: Lncrnas In Joint Destruction Of Ramentioning

confidence: 99%

The effect of long non-coding RNAs in joint destruction of rheumatoid arthritis

Zhao

et al. 2022

Front. Cell Dev. Biol.

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Rheumatoid arthritis (RA) is a systemic autoimmune disease accompanied with joint destruction. Serious joint destruction will eventually lead to disability and the decline of life quality in RA patients. At present, the therapeutic effect of drugs to alleviate joint destruction in RA is limited. Recently, accumulating evidences have shown that long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of joint diseases. Therefore, this paper reviews the expression change and the action mechanism of lncRNAs in joint destruction of RA in recent years. A more comprehensive understanding of the role of lncRNAs in joint destruction will help the treatment of RA.

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LncRNA-mRNA Expression Profiles of Osteoclast After Conditional Knockout HIF-1α

Cited by 3 publications

References 43 publications

lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis

lncRNA-Gm5532 regulates osteoclast differentiation through the miR-125a-3p/TRAF6 axis

HIF-1α Regulates Bone Homeostasis and Angiogenesis, Participating in the Occurrence of Bone Metabolic Diseases

The effect of long non-coding RNAs in joint destruction of rheumatoid arthritis

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