LncRNA MALAT1 sponges miR-204 to promote osteoblast differentiation of human aortic valve interstitial cells through up-regulating Smad4

Xiao, Xiaoxiong; Zhou, Tingwen; Guo, Shichao; Guo, Chao; Qiao, Zhengxue; Dong, Nianguo; Wang, Yongjun

doi:10.1016/j.ijcard.2017.05.037

Cited by 139 publications

(106 citation statements)

References 46 publications

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“…Similar regulatory mechanisms have been identified for other lncRNAs in osteogenic differentiation. For example, lncRNA MALA1 promoted the osteogenic differentiation of aortic valve interstitial cells by sponging miR‐204, and miR‐204's target gene, Smad4 , was up‐regulated . LncRNA H19 promoted BMSC osteoblast differentiation by targeting miR‐675, and the down‐regulation of miR‐675 subsequently elevated TGF‐β1 mRNA and protein expressions .…”

Section: Discussionmentioning

confidence: 99%

LncRNA‐PCAT1 targeting miR‐145‐5p promotes TLR4‐associated osteogenic differentiation of adipose‐derived stem cells

et al. 2018

J Cellular Molecular Medi

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This study was aimed to explore the differential expression of long noncoding RNAs (lncRNA)‐PCAT1, miR‐145‐5p and TLR4 in osteogenic differentiation via the Toll‐like receptor (TLR) signalling pathway and consequently determine the potential molecular mechanism. The mRNAs and pathways related to the osteogenic differentiation in human adipose‐derived stem cells (hADSCs) were analysed by bioinformatics. The MiRanda and TargetScan database were employed to detect the potential binding sites of miRNAs on lncRNAs and mRNAs. The differential expression of lncRNA‐PCAT1, miR‐145‐5p and TLR4 were detected by qRT‐PCR. Rrelated protein expression was analysed by Western blot. The targeted relationships between lncRNA‐PCAT1, miR‐145‐5p and TLR4 were verified by dual‐luciferase reporter assay. Alkaline phosphatase (ALP) activity and ARS staining assays were used to measure the impacts exerted by lncRNA PCAT1, miR‐145‐5p and TLR4 mRNA on osteogenic differentiation. After the induction of osteoblast differentiation, the expression of lncRNA‐PCAT1 and TLR4 increased, while the expression of miR‐145‐5p decreased. Dual‐luciferase reporter assay confirmed the targeted relationship between lncRNA‐PCAT1, miR‐145‐5p, and TLR4. LncRNA‐PCAT1 negatively regulated miR‐145‐5p and positively regulated TLR4. Knockdown of lncRNA‐PCAT1 or TLR4 decreased the expression of osteogenic differentiation‐related proteins, reduced the ALP and ARS levels and the activity of the TLR signalling pathway. MiR‐145‐5p could reverse the effects of PCAT1 and TLR4 in hADSCs osteogenic differentiation. LncRNA‐PCAT1 negatively regulated miR‐145‐5p, which promoted TLR4 expression to promote osteogenic differentiation by activating the TLR signalling pathway.

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Section: Discussionmentioning

confidence: 99%

LncRNA‐PCAT1 targeting miR‐145‐5p promotes TLR4‐associated osteogenic differentiation of adipose‐derived stem cells

et al. 2018

J Cellular Molecular Medi

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“…Additionally, a novel post-transcriptional regulatory mechanism of RNAs has been reported in which lncRNAs crosstalk with miRNAs through competing, functioning as competing endogenous RNAs and thus modulating the derepression of miRNAs on their targets [55]. For instance, Xiao et al demonstrate that LncRNA MALAT1 may function as a competing endogenous RNAs to up-regulate Smad4 expression by sponging miR-204, promoting osteoblast differentiation of human aortic valve interstitial cells [28]. Furthermore, it has been found that LncRNA MEG3 directly targeting miR-133a-3p to inhibit the bone marrow mesenchymal stem cells differentiation in osteoporosis [56].…”

Section: Discussionmentioning

confidence: 99%

“…The expression profile of lncRNAs is tissue-specific and differentially expressed across various stages of cell differentiation [26]. Recently, it has been reported that lncRNAs play an important role in regulating bone development and remodeling, however, most of the functions of lncRNAs in these processes still remain unclear [27, 28]. The regulating function of lncRNAs is not solitary but by the diverse and complex mechanism with the participation of DNA, microRNAs, mRNAs, and proteins [29-31].…”

Section: Introductionmentioning

confidence: 99%

“…Similar with lncRNAs, microRNAs, which are non-coding small RNAs composed of approximately 22 nucleotides, are also involved in controlling bone development and remodeling, including osteoclastogenesis [32-34]. In recent studies, an interesting point of view has been presented that lncRNAs are regarded as the role of sponges to make microRNAs exhausted, thus preventing mRNA from degradation by microRNA [28, 35]. In spite of these promising findings, the recognition of lncRNAs which play a vital role in different stages during osteoclastogenesis and the detailed mechanism of the genetic pathways remains to be investigated.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells

Quan

Liang

et al. 2018

Cell Physiol Biochem

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Background/Aims: In the process of bone development and remodeling, the vasculature is regarded as the communicative network between the bone and neighboring tissues. Recently, it has been reported that the processes of angiogenesis and osteogenesis are coupled temporally and spatially. However, few studies reported the relationship and relevant mechanism between osteoclastogenesis and vasculogenesis. Methods: Arraystar Mouse lncRNA microarray V3.0 was firstly used to analyze the differentially expressed lncRNA genes in osteoclast different stages during osteoclastogenesis. Cell counting kit 8 (CCK-8) analysis, quantitative real-time polymerase chain reaction (qRT-PCR) analysis, migration and tube formation assays were used to detect impact of osteoclast different stages on the proliferation, differentiation, migration and tube formation of endothelial progenitor cells (EPCs), respectively. Finally, transfection of AK131850 shRNA, miR-93-5p mimic and miR-93-5p inhibitor, qRT-PCR, western blotting, enzyme-linked immunosorbent assay (ELISA), fluorescence in situ hybridization (FISH) and luciferase reporter assay were carried out to dissect molecular mechanisms. Results: In this study, we found that newborn OCs (N-OC) and mature OCs (M-OC) during osteoclastogenesis significantly promoted proliferation, differentiation, migration and tube formation of endothelial progenitor cells (EPCs). Through lncRNA microarray and GO&pathway analysis, we found that AK131850 and co-expressed gene, vascular endothelial growth factor a (VEGFa), were significantly up-regulated in N-OC and M-OC. After inhibition of AK131850 the promoting effect of N-OC and M-OC on EPCs was reversed. Furthermore, we found that AK131850 directly competed miR-93-5p in N-OC and M-OC through sponge, thereby increasing VEGFa transcription, expression and secretion through derepressing of miR-93-5p on VEGFa. Conclusion: Our results provided the first finding that lncRNA-AK131850 sponged miR-93-5p in N-OC and M-OC during osteoclastogenesis to enhance the secretion of VEGFa, thus promoting vasculogenesis of EPCs.

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“…However, the conclusion drawn by Chen et al should be further verified for the following reasons. Firstly, although MALAT1 is almost always retained in the nucleus (30) where it is localized within nuclear speckles (31,32), studies have reported MALAT1 interaction with miRNA in the cytoplasm (21,27,33). Therefore, the subcellular localization of MALAT1 should be verified in HepG2 cells.…”

mentioning

confidence: 99%

Metastasis-associated lung adenocarcinoma transcript 1 regulates tumor progression: old wine in a new bottle

Yan

Kuang

et al. 2018

J. Thorac. Dis.

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LncRNA MALAT1 sponges miR-204 to promote osteoblast differentiation of human aortic valve interstitial cells through up-regulating Smad4

Cited by 139 publications

References 46 publications

LncRNA‐PCAT1 targeting miR‐145‐5p promotes TLR4‐associated osteogenic differentiation of adipose‐derived stem cells

LncRNA‐PCAT1 targeting miR‐145‐5p promotes TLR4‐associated osteogenic differentiation of adipose‐derived stem cells

LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells

Metastasis-associated lung adenocarcinoma transcript 1 regulates tumor progression: old wine in a new bottle

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